Analysis of Enterococcus faecalis isolates from intercontinental sources by multilocus enzyme electrophoresis and pulsed-field gel electrophoresis.

1995 ◽  
Vol 33 (11) ◽  
pp. 2903-2907 ◽  
Author(s):  
J F Tomayko ◽  
B E Murray
Keyword(s):  
Enterococcus Faecalis ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Enzyme Electrophoresis ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis

scholarly journals Genetic characterization ofMycobacterium aviumisolates recovered from humans and animals in Australia

1996 ◽  
Vol 116 (1) ◽  
pp. 41-49 ◽  
Author(s):  
M. M. Feizabadi ◽  
I. D. Robertson ◽  
D. V. Cousins ◽  
D. Dawson ◽  
W. Chew ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Avium ◽  
Gel Electrophoresis ◽  
Genetic Characterization ◽  
Genetic Relationships ◽  
Pulsed Field Gel Electrophoresis ◽  
Pfge Type ◽  
Enzyme Electrophoresis ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis

SummaryGenetic relationships amongst 115 mainly Australian isolates ofMycobacterium aviumwere assessed using multilocus enzyme electrophoresis (MEE). The isolates were divided into 58 electrophoretic types (ETs), with a mean genetic diversity of 0·29. Isolates from humans were closely related to but distinct from those cultured from birds, whilst some porcine isolates belonged to the same ETs as certain human isolates. Pulsed field gel electrophoresis (PFGE) was used to differentiate related isolates, and those from birds and some from other animals, including pigs, were distinguished from the human isolates. The results of MEE and PFGE suggested that certain strains ofM. aviummay be transmitted between birds and pigs, but there was no clear evidence of transmission to humans. The serovar of theM. aviumisolates was not obviously related to their ET assignment or their PFGE type.

Disparity between Multilocus Enzyme Electrophoresis, Microsatellite Markers and Pulsed-Field Gel Electrophoresis in epidemiological tracking of Candida albicans

2010 ◽  
Vol 82 (3) ◽  
pp. 265-281 ◽  
Author(s):  
Marcelo Fabiano Gomes Boriollo ◽  
Ricardo Antunes Dias ◽  
João Evangelista Fiorini ◽  
Nelma de Mello Silva Oliveira ◽  
Denise Madalena Palomari Spolidório ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Microsatellite Markers ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Enzyme Electrophoresis ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis

scholarly journals Staphylococcus aureus strains of type 95. Spread of a single clone

1994 ◽  
Vol 113 (3) ◽  
pp. 463-470 ◽  
Author(s):  
V. T. Rosdahl ◽  
W. Witte ◽  
M. Musser ◽  
J. O. Jarløv
Keyword(s):  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Enzyme Electrophoresis ◽  
Restriction Fragments ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis ◽  
Single Clone ◽  
Clonal Origin ◽  
Reference Strains

SUMMARYStaphylococcus aureus strains of type 95 in Denmark have increased to a frequency of 20% of the total S. aureus population. A clonal origin and possible subdivision of these strains have been discussed. In the present investigation 35 epidemiologically unrelated S. aureus strains of type 95 as well as reference strains of other types have been analysed by other typing techniques including lectintyping, multilocus enzyme electrophoresis and pulsed-field gel electrophoresis of genomic restriction fragments. No subdivision could be achieved based on any of these methods and a clonal origin seems therefore possible.

scholarly journals Draft Genome Sequences of the Probiotic Enterococcus faecalis Symbioflor 1 Clones DSM16430 and DSM16434

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Moritz Fritzenwanker ◽  
Anindita Chakraborty ◽  
Torsten Hain ◽  
Kurt Zimmermann ◽  
Eugen Domann
Keyword(s):  
Comparative Analysis ◽  
Enterococcus Faecalis ◽  
Gel Electrophoresis ◽  
Draft Genome ◽  
Pulsed Field Gel Electrophoresis ◽  
Genome Sequences ◽  
Pulsed Field

The probiotic Symbioflor 1 is a historical concoction of 10 isolates of Enterococcus faecalis . Pulsed-field gel electrophoresis revealed two groups: one comprising eight identical clones (DSM16430, DSM16432, DSM16433, DSM16435 to DSM16439) and a further two isolates (DSM16431, DSM16434) with marginally different profiles. Here, we report a comparative analysis of the draft genome sequences of representative isolates.

scholarly journals Comparison of Multilocus Variable-Number Tandem-Repeat Analysis with Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis for Enterococcus faecalis

2011 ◽  
Vol 60 (4) ◽  
pp. 335-339 ◽  
Author(s):  
EWA SADOWY ◽  
ALEKSANDRA SIEŃKO ◽  
WALERIA HRYNIEWICZ
Keyword(s):  
Enterococcus Faecalis ◽  
Gel Electrophoresis ◽  
Tandem Repeats ◽  
Diversity Index ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Pulsed Field Gel Electrophoresis ◽  
Discriminatory Power ◽  
Pulsed Field ◽  
High Discriminatory Power

Enterococcus faecalis represents recently an important etiological agent of health care-associated infections (HAIs) and there is a need for evaluation and comparison of typing methods available for this microorganism. We tested multilocus VNTR (variable-number tandem repeats) analysis (MLVA) on a well-characterized collection of 153 clinical isolates of E. faecalis, corresponding to 52 multilocus sequence types and 67 pulsed-field gel electrophoresis (PFGE) profiles. MLVA showed high discriminatory power, discerning 111 different types (diversity index equal 98.9%). The concordance MLVA/MLST and MLVA/PFGE was 0.95 and 0.74, respectively. High discriminatory power of MLVA indicates its utility for local epidemiology such as outbreak investigation, and for differentiation of clones defined by other methods.

scholarly journals Local Genetic Patterns within a Vancomycin-Resistant Enterococcus faecalis Clone Isolated in Three Hospitals in Portugal

2004 ◽  
Vol 48 (9) ◽  
pp. 3613-3617 ◽  
Author(s):  
Carla Novais ◽  
Teresa M. Coque ◽  
João Carlos Sousa ◽  
Fernando Baquero ◽  
Luisa Peixe
Keyword(s):  
Enterococcus Faecalis ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Vancomycin Resistant Enterococcus ◽  
Pulsed Field ◽  
Genetic Patterns ◽  
Single Clone ◽  
Vancomycin Resistant

ABSTRACT Eight pulsed-field gel electrophoresis subtypes and six Tn1546 variants were identified among Enterococcus faecalis isolates of a single clone recovered in three geographically separate Portuguese hospitals. Some clonal subtypes were found in particular hospitals, and Tn1546 variants were either widespread or confined to some of them. We also report on the first Tn1546 transposon containing an ISEf1 insertion.

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