Molecular surveillance ofNeisseria meningitidiscapsular switching in Portugal, 2002–2006

SUMMARYNeisseria meningitidiscapsular switching has been reported in several countries. In order to establish the genetic relationship within group B and C strains expressing subtypes 2a or 2b, and to evaluate whether C to B capsular switching occurred in Portugal, 64 meningococci (56 serogroup C and 8 serogroup B) isolated from invasive meningococcal disease were typed using molecular methods. The studied phenotypes, 2b:P1.5,2 and 2a:P1.5-1,10-8, were the most frequent among serogroup C, but were uncommon among serogroup B strains. The multi-locus sequence typing (MLST) allelic profile and the pulsed-field gel electrophoresis (PFGE) fingerprints showed that seven serogroup B strains were genotypically identical to C strains, suggesting that capsular switching occurred. Active laboratory surveillance to find evidence of capsule switching is a now priority as MenC was introduced in the Portuguese vaccination schedule in January 2006.

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Evaluation of Pulsed-Field Gel Electrophoresis in Epidemiological Investigations of Meningococcal Disease Outbreaks Caused by Neisseria meningitidis Serogroup C

Journal of Clinical Microbiology ◽

10.1128/jcm.39.1.75-85.2001 ◽

2001 ◽

Vol 39 (1) ◽

pp. 75-85 ◽

Cited By ~ 52

Author(s):

T. Popovic ◽

S. Schmink ◽

N. A. Rosenstein ◽

G. W. Ajello ◽

M. W. Reeves ◽

...

Keyword(s):

Neisseria Meningitidis ◽

Meningococcal Disease ◽

Gel Electrophoresis ◽

Disease Outbreaks ◽

Pulsed Field Gel Electrophoresis ◽

Pulsed Field

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Meningococcal Group B Vaccine For The Prevention Of Invasive Meningococcal Disease Caused By Neisseria meningitidis Serogroup B

Infection and Drug Resistance ◽

10.2147/idr.s159952 ◽

2019 ◽

Vol Volume 12 ◽

pp. 3169-3188 ◽

Cited By ~ 2

Author(s):

Irene Rivero-Calle ◽

Peter Francis Raguindin ◽

Jose Gómez-Rial ◽

Carmen Rodriguez-Tenreiro ◽

Federico Martinón-Torres

Keyword(s):

Neisseria Meningitidis ◽

Meningococcal Disease ◽

Invasive Meningococcal Disease ◽

Meningococcal Group ◽

Group B

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Pharyngeal carriage of Neisseria meningitidis and Neisseria lactamica in households with infants within areas with high and low incidences of meningococcal disease

Epidemiology and Infection ◽

10.1017/s0950268800067492 ◽

1991 ◽

Vol 106 (3) ◽

pp. 445-457 ◽

Cited By ~ 37

Author(s):

S. F. Olsen ◽

B. Djurhuus ◽

K. Rasmussen ◽

H. D. Joensen ◽

S. O. Larsen ◽

...

Keyword(s):

Confidence Interval ◽

Neisseria Meningitidis ◽

Meningococcal Disease ◽

Odds Ratio ◽

Lower Risk ◽

Household Survey ◽

Faroe Islands ◽

Odds Ratios ◽

Neisseria Lactamica ◽

Group B

SUMMARYIn a household survey in the Faroe Islands, an isolated community with hyperendemic occurrence of meningococcal disease due to serogroup B 15, 1604 persons were examined for pharyngeal carriage of Neisseria meningitidis and N. lactamica. Two areas were chosen having experienced high (HIA), and two having experienced low incidences (LIA) of disease. Living in HIA compared with LIA was associated with higher risk of N. meningitidis B 15 carriage and lower risk of N. lactamica carriage, with odds ratios of 2·7 (95% confidence interval (CI) 1·4–5·1, P = 0·003) and 0·41 (95% CI 0·31–0·53, P < 0·0001), respectively. In HIA the risk of N. meningitidis carriage was much lower in non-carriers than carriers of N. lactamica, with an odds ratio of 0·19 (95% CI 0·08–0·47, P = 0·0003); in LIA this association (odds ratio 0·51, P = 0·05) was much weaker. Children 0–14 years had substantially higher risk of being carriers of N. meningitidis group B 15 if the mothers were so, with an odds ratio of 11 (95% CI 4–29, P < 0·0001).

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Typing of Group B Streptococci by Pulsed-Field Gel Electrophoresis

Kansenshogaku zasshi ◽

10.11150/kansenshogakuzasshi1970.69.455 ◽

1995 ◽

Vol 69 (4) ◽

pp. 455-460 ◽

Cited By ~ 2

Author(s):

Daisuke TANAKA ◽

Yotaku GYOBU ◽

Hirohide KODAMA

Keyword(s):

Gel Electrophoresis ◽

Pulsed Field Gel Electrophoresis ◽

Group B Streptococci ◽

Pulsed Field ◽

Group B

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Usefulness of Pulsed-Field Gel Electrophoresis in Tracking Two Outbreaks of Invasive Meningococcal Disease Serogroup C in British Columbia

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2007/295973 ◽

2007 ◽

Vol 18 (6) ◽

pp. 363-367 ◽

Cited By ~ 3

Author(s):

Grahame Quan ◽

Mark Gilbert ◽

Samara T David ◽

Tazim Rahim ◽

Kathy Adie ◽

...

Keyword(s):

British Columbia ◽

Meningococcal Disease ◽

Gel Electrophoresis ◽

Outer Membrane Proteins ◽

Case Fatality ◽

Pulsed Field Gel Electrophoresis ◽

Invasive Meningococcal Disease ◽

Sequencing Data ◽

Outbreak Strain ◽

Pulsed Field

Two major outbreaks of invasive meningococcal disease serogroup C (IMD-C) were identified in British Columbia between 2000 and 2004. Pulsed-field gel electrophoresis (PFGE) andporAgene sequencing of all retained IMD-C isolates were used to assess correlations between genotypes and epidemiological patterns. PFGE patterns of IMD-C genotypes correlated with epidemiological patterns between 2000 and 2004 in British Columbia, and demonstrated that PFGE can identify outbreak-related cases. Both IMD-C outbreaks correlated with a respective PFGE pattern. PFGE analysis demonstrated that the 2004 British Columbia outbreak strain in men who have sex with men was closely related to the 2001 Abbotsford outbreak strain.PorAsequencing data indicated low diversity of class 1 outer membrane proteins in British Columbia, and did not correlate with epidemiological trends. There was a trend for outbreak-associated PFGE types to demonstrate higher case fatality rates.

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Potential Capsule Switching from Serogroup Y to B: The Characterization of Three suchNeisseria meningitidisIsolates Causing Invasive Meningococcal Disease in Canada

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2005/216369 ◽

2005 ◽

Vol 16 (3) ◽

pp. 171-174 ◽

Cited By ~ 15

Author(s):

Raymond SW Tsang ◽

Dennis KS Law ◽

Shaun D Tyler ◽

Gwen S Stephens ◽

Mark Bigham ◽

...

Keyword(s):

Neisseria Meningitidis ◽

Meningococcal Disease ◽

Multilocus Sequence Typing ◽

Housekeeping Gene ◽

Sequence Type ◽

Invasive Meningococcal Disease ◽

Group B

Three group BNeisseria meningitidisisolates, recovered from meningococcal disease cases in Canada and typed as B:2c:P1.5, were characterized. Multilocus sequence typing showed that all three isolates were related because of an identical sequence type (ST) 573. Isolates typed as 2c:P1.5 are common in serogroup Y meningococci but rare in isolates from serogroups B or C. Although no serogroup Y isolates have been typed as ST-573, eight isolates showed five to six housekeeping gene alleles that were identical to that of ST-573. This suggested that the B:2c:P1.5 isolates may have originated from serogroup Y organisms, possibly by capsule switching.

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Molecular Epidemiology of Recent Belgian Isolates of Neisseria meningitidis Serogroup B

Journal of Clinical Microbiology ◽

10.1128/jcm.36.10.2828-2834.1998 ◽

1998 ◽

Vol 36 (10) ◽

pp. 2828-2834 ◽

Cited By ~ 11

Author(s):

M. Van Looveren ◽

P. Vandamme ◽

M. Hauchecorne ◽

M. Wijdooghe ◽

F. Carion ◽

...

Keyword(s):

Neisseria Meningitidis ◽

Gel Electrophoresis ◽

Epidemiological Data ◽

Pulsed Field Gel Electrophoresis ◽

Enzyme Electrophoresis ◽

Clin Microbiol Infect ◽

Epidemic Clone ◽

Multilocus Enzyme Electrophoresis ◽

Repetitive Motif ◽

Reference Strains

In Belgium an increase in the incidence of meningococcal disease has been noted since the early 1990s. Four hundred twenty clinical strains isolated during the period from 1990 to 1995, along with a set of 30 European reference strains, and 20 Dutch isolates were examined by random-primer and repetitive-motif-based PCR. A subset was investigated by multilocus enzyme electrophoresis and pulsed-field gel electrophoresis. The data were compared with results obtained by serotyping (M. Van Looveren, F. Carion, P. Vandamme, and H. Goossens, Clin. Microbiol. Infect. 4:224–228, 1998). Both phenotypic and molecular epidemiological data suggest that the lineage III ofNeisseria meningitidis, first encountered in The Netherlands in about 1980, has been introduced in Belgium. The epidemic clone, as defined by oligonucleotide D8635-primed PCR, encompasses mainly phenotypes B:4:P1.4 and B:nontypeable:P1.4, but strains with several other phenotypes were also encountered. Therefore, serotyping alone would underestimate the prevalence of the epidemic clone.

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