Ectopic autologous transplantation of ovarian tissue as a feasible technique to assess ovarian morphophysiology

Zygote ◽  
2021 ◽  
pp. 1-3
Author(s):  
Bárbara Rodrigues Nascimento ◽  
Guilherme Antonio de Gouvêa Lopes ◽  
Danielle Storino de Freitas ◽  
Paulo Henrique Almeida Campos-Junior
Keyword(s):  
Ovarian Function ◽  
Ovarian Tissue ◽  
Autologous Transplantation ◽  
Postoperative Recovery ◽  
Dorsal Region ◽  
Promising Technique ◽  
Effective Technique ◽  
Short Article ◽  
Preservation Of Fertility ◽  
Near Future

Summary The cryopreservation of murine ovarian tissue and its transplantation can be a promising technique for the preservation of fertility and an alternative for the future reconstitution of scientific valuable strains of mice. Accordingly, the aim of this study was to describe the entire surgical procedure for ovariectomy and dorsal subcutaneous autotransplantation in mice, and also some data about the efficiency of this procedure. Female C57Bl/6J mice (n = 18) were anaesthetised and bilaterally ovariectomized. After surgery, ovaries were autotransplanted in small subcutaneous pouches in the dorsal region of the forelimbs. The animals were inspected daily and, 23 days after transplantation, euthanasia and recovery of ovarian tissues were performed. Postoperative recovery, oestrous cyclicity, and folliculogenesis progression were evaluated. At 23 days after transplantation, the recovery of the ovaries was feasible, all classes (primordial to antral) of follicles were observed. Additionally, satisfactory efficiency rates were obtained, with 100% of anaesthesia survival rate, survival, graft recovery, folliculogenesis progression and oestrous cyclicity. In general, this short article describes ovarian ectopic autologous transplantation as an effective technique for maintaining rodent oogenesis and endocrine ovarian function. Even more broadly, we can still assume that the application of this technique may reduce the number of breeding matrices and experimental animals in the near future.

Rat modeling of ovarian tissue cryopreservation and auto-grafting

2019 ◽  
Author(s):  
◽  
Tareq Mohamed Elhadi Lehmidi
Keyword(s):  
Ovarian Function ◽  
Ovarian Tissue ◽  
Autologous Transplantation ◽  
General Medicine ◽  
Human Reproduction ◽  
Ovarian Tissue Cryopreservation ◽  
University Of Missouri ◽  
Life Saving ◽  
The University ◽  
Tissue Cryopreservation

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] New advancements in cancer treatment methods are increasing the survival rate of cancer patients. Unfortunately, these life-saving treatments, such as chemotherapy and radiotherapy, have permanent impacts on ovarian function leading to infertility, osteoporosis and obesity. Autologous transplantation of previously cryopreserved ovarian tissues would serve as a potential therapeutic approach in human reproduction and general medicine in terms of restoring steroid hormone function, fertility, body composition, and bone integrity.

scholarly journals Cryopreservation and Fertility: Current and Prospective Possibilities for Female Cancer Patients

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Jacira Ribeiro Campos ◽  
Ana Carolina Japur de Sá Rosa-e-Silva
Keyword(s):  
Ovarian Function ◽  
Ovarian Tissue ◽  
Survival Rates ◽  
Reproductive Function ◽  
Human Reproduction ◽  
Malignant Neoplasms ◽  
Assisted Human Reproduction ◽  
Female Cancer Patients ◽  
Preservation Of Fertility

With the evolution of the treatment of malignant neoplasms, the survival rates of patients undergoing chemo- or radiotherapy are increasing. The continuous development of techniques of assisted human reproduction has led to important strategies in an attempt to maintain reproductive function in patients subjected to treatment of neoplastic diseases, among them cryopreservation of embryos, gametes, and ovarian cortical tissue. The freezing of ovarian tissue is currently being proposed with the primary purpose of preserving ovarian function in these patients. Currently, the major challenge of groups working with preservation of fertility is the use of cryopreserved ovarian tissue after disease remission. The main alternatives presented today are the implantation of hetero- or orthotopic tissue and isolation of immature follicles from ovarian tissue followed by in vitro maturation and assisted reproduction procedures.

scholarly journals Ovarian tissue freezing and activation after thawing: an update

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Li-fan Peng
Keyword(s):  
Granulosa Cells ◽  
Clinical Decision Making ◽  
Ovarian Function ◽  
Ovarian Tissue ◽  
Clinical Decision ◽  
The Body ◽  
Endocrine Function ◽  
Ovarian Tissue Cryopreservation ◽  
Main Body ◽  
Tissue Cryopreservation

Abstract Background With the growth of women’s age, ovarian failure can be caused by various factors. For the women who need chemotherapy because of cancer factors, the preservation of fertility is more urgent. The treatment of cancer is also a process in which all tissues and organs of the body are severely damaged, especially in the reproductive system. Main body As a new fertility preservation technology, autologous ovarian tissue cryopreservation and transplantation is developing rapidly and showing great potentiality in preserving ovarian endocrine function of young cervical cancer patients. Vitrification and slow freezing are two common techniques applied for ovarian tissue cryopreservation. Thus, cryopreserved/thawed ovarian tissue and transplantation act as an important method to preserve ovarian function during radiotherapy and chemotherapy, and ovarian cryopreservation by vitrification is a very effective and extensively used method to cryopreserve ovaries. The morphology of oocytes and granulosa cells and the structure of organelles were observed under the microscope of histology; the hormone content in the stratified culture medium of granulosa cells with the diameter of follicle was used to evaluate the development potential of ovarian tissue, and finally the ovarian tissue stimulation was determined by the technique of ovarian tissue transplantation. Conclusions Although there are some limitations, the team members still carry out this review to provide some references and suggestions for clinical decision-making and further clinical research.

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