Increasing Nitric Oxide Synthase in Human Renal Graft Rejection after Kidney Transplantation

Nitric oxide (NO) is a potent endothelial-drived vasorelaxing factor and may involved in salt sensitive hypertension and renal damage. Nitric oxide is produced from L-arginine by activation of nitric oxide synthase (NOS). Three NOS has been identified which are brain NOS (bNOS), inducible NOS (iNOS) and endothelial NOS (eNOS). On the other hand, renal graft rejection is major problem after kidney transplantation with severe renal damage and renal vasoconstriction. While NOS has been reported localized in human and animal kidney, the status of three NOS in human renal tissue with rejection remains poorly defined. Therefore, the present study was designed to determine the expression of bNOS, iNOS and eNOS by immunohistochemical staining (IHCS) in human renal tissue with rejection and compared with that in normal human renal tissue.Human renal biopsy (n=5) were obtained after kidney transplantation with mild and moderate renal rejection. Normal kidney biopsy was obtained during nephrectomy.

Download Full-text

Enhancement of Natriuretic Peptides in Rejected Human Renal Graft

Microscopy and Microanalysis ◽

10.1017/s1431927600035509 ◽

2000 ◽

Vol 6 (S2) ◽

pp. 602-603

Author(s):

H. Song ◽

J. Papadimitriou ◽

C. Drachenberg ◽

M. R. Weir ◽

C. Wei

Keyword(s):

Kidney Transplantation ◽

Natriuretic Peptide ◽

Natriuretic Peptides ◽

Graft Rejection ◽

Tissue Level ◽

Renal Tissue ◽

Compensatory Mechanism ◽

Renal Vasoconstriction ◽

Renal Graft ◽

Renal Graft Rejection

Natriuretic peptides include atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and C-type natriuretic peptide (CNP). ANP, BNP and CNP are structure related but genetic distinct 1 natriuretic peptide family. ANP and BNP are cardiac cell origin and CNP are endothelial cell and kidney origin. Natriuretic peptides have potent vasoactive and natriuretic actions through generation of cGMP. On the other hand, renal graft rejection is major problem after kidney transplantation with severe renal damage and renal vasoconstriction. We hypothesized that renal tissue level of natriuretic peptides increase in renal graft rejection through compensatory mechanism. Therefore, the present study was designed to determine the expression of natriuretic peptides by immunohistochemical staining (IHCS) in human renal tissue with rejection and compare with normal renal tissue.Human renal biopsy (n=5) were obtained after kidney transplantation with mild and moderate renal rejection. Normal kidney biopsy was obtained during nephrectomy. ANP, BNP and CNP levels in renal tissue were determined by IHCS.

Download Full-text

Enhancement of Apoptosis-Related Gene Expression in Human Renal Graft Rejection

Microscopy and Microanalysis ◽

10.1017/s1431927600035625 ◽

2000 ◽

Vol 6 (S2) ◽

pp. 626-627

Author(s):

P. Y. Lau ◽

J. Papadimitriou ◽

C. Drachenberg ◽

M. R. Weir ◽

C. Wei

Keyword(s):

Gene Expression ◽

Kidney Transplantation ◽

Graft Rejection ◽

Human Kidney ◽

Renal Tissue ◽

Related Gene ◽

Renal Graft ◽

Normal Human ◽

Apoptosis Related Gene ◽

Renal Graft Rejection

Apoptosis or programmed cell death is involved in many diseases include end-stage renal failure. Apoptosis-related genes include both stimulate genes and inhibitory gene of apoptosis. The genes which stimulate apoptosis include p53 and p21-WAF. The genes which inhibit apoptosis include bcl-2 gene family. The mechanisms of apoptosis include p53-dependend pathway and p53- independent pathway. We hypothesized that apoptosis-related genes may activate in renal graft rejection after kidney transplantation. Therefore, the present study was designed to investigate apoptosis-related gene expression and localization by immunohistochemical staining (IHCS) in human renal tissues with graft rejection and compare with that in normal human renal tissue.Human renal biopsy (n=5) were obtained after kidney transplantation with mild and moderate renal rejection. Normal human kidney biopsy was obtained during nephrectomy. P53, p21-WAF and Bcl-2 levels in renal tissue were determined by IHCS. The results of IHCS was evaluated by IHCS staining density scores (0, no staining; 1, minimal staining; 2, mild staining; 3, moderate staining; and 4, strong staining).

Download Full-text