RNAi effects on regulation of endogenous acid invertase activity in potato (Solanum tuberosum L.) tubers

2008 ◽  
Vol 5 (2) ◽  
pp. 107-112 ◽  
Author(s):  
Zhang Chi ◽  
Xie Cong-Hua ◽  
Song Bo-Tao ◽  
Liu Xun ◽  
Liu Jun
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Pcr Amplification ◽  
Invertase Activity ◽  
Northern Blotting ◽  
Acid Invertase ◽  
Transcriptional Gene Silencing ◽  
Post Transcriptional Gene Silencing ◽  
Transgenic Lines ◽  
Potential Strategy

AbstractIn plants, acid invertases are known to be the key enzymes cleaving sucrose into reducing sugars (RS) (glucose and fructose). To improve the quality of potato (Solanum tuberosum L.) chips, which is largely influenced by RS accumulation in tubers stored at low temperature, a part of acid invertase cDNA with hairpin RNA (hpRNA) structure was transformed into potato cv. N2. Detection of polymerase chain reaction (PCR) amplification and Northern blotting suggested that the RNA interference (RNAi) vector was successfully transformed into cv. N2. The analysis of acid invertase activity in the plantlets and microtubers of RNAi transgenic lines indicated that the expression of the acid invertase was significantly repressed by the activity of RNAi of plantlets by an average 69.8% (with the exception of line Ni-1) with a maximal decrease of 78% (line Ni-4), and the highest decrease of activity in microtubers of 68%. Compared with that of well-inhibited antisense inv transgenic plants, the comparative downregulation of RNAi suggests a distinct alteration of endogenous acid invertase activity and a potential strategy for post-transcriptional gene silencing (PTGS) in modulation of cold-sweetening in potato.

scholarly journals Homology-Dependent Virus Resistance in Transgenic Plants with the Coat Protein Gene of Sweet Potato Feathery Mottle Potyvirus: Target Specificity and Transgene Methylation

1999 ◽  
Vol 89 (5) ◽  
pp. 385-391 ◽  
Author(s):  
S. Sonoda ◽  
M. Mori ◽  
M. Nishiguchi
Keyword(s):  
Coat Protein ◽  
Sweet Potato ◽  
Virus Resistance ◽  
Potato Virus X ◽  
Transcriptional Gene Silencing ◽  
Target Specificity ◽  
Post Transcriptional Gene Silencing ◽  
Resistant Lines ◽  
Nontranslated Region ◽  
Transgenic Lines

Nicotiana benthamiana plants were transformed with the coat protein (CP) coding sequence and the 3′ nontranslated region (NTR) of the severe strain of sweet potato feathery mottle potyvirus (SPFMV-S). Regenerated lines were screened for virus resistance using recombinant potato virus X (PVX) engineered to contain the sequence homologous to the transgene. Out of 19 transgenic lines, 7 showed virus resistance after inoculation by the recombinant PVX. In most of the resistant lines, relatively low steady-state accumulation of the CP gene mRNA and little or no protein products were observed, suggesting that the resistance was manifested by a post-transcriptional gene-silencing mechanism. The resistant lines could be divided into two groups according to the target specificity of the silencing mechanism; one group recognizing the 3′ part of the transgene mRNA and the other not only the 3′ part, but also the 5′ and the central part of the transgene mRNA. Particular regions of the transgene corresponding to the RNA target in the resistant lines were differentially methylated compared with the transgene sequence in a susceptible line.

scholarly journals Amylase and Invertase Activity and Substrate Affinity in 'Danshaku' Potato Tubers (Solanum tuberosum L.) During Cold Storage

2000 ◽  
Vol 26 (5) ◽  
pp. 281-287
Author(s):  
Yosuke MURATA ◽  
Tomohiro NOGUCHI ◽  
Kevin F. YAPTENCO ◽  
Hiroaki SATO ◽  
Shinji MATSUMOTO ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Cold Storage ◽  
Solanum Tuberosum L ◽  
Invertase Activity ◽  
Substrate Affinity ◽  
Potato Tubers

scholarly journals Double-Virus Resistance of Transgenic Oriental Melon Conferred by Untranslatable Chimeric Construct Carrying Partial Coat Protein Genes of Two Viruses

Plant Disease ◽  
2010 ◽  
Vol 94 (11) ◽  
pp. 1341-1347 ◽  
Author(s):  
Hui-Wen Wu ◽  
Tsong-Ann Yu ◽  
Joseph A. J. Raja ◽  
Serene Judith Christopher ◽  
Sine-Lan Wang ◽  
...  
Keyword(s):  
Coat Protein ◽  
Virus Resistance ◽  
Zucchini Yellow Mosaic Virus ◽  
Inverse Correlation ◽  
Underlying Mechanism ◽  
Yellow Mosaic Virus ◽  
Transcriptional Gene Silencing ◽  
Oriental Melon ◽  
Post Transcriptional Gene Silencing ◽  
Transgenic Lines

Production of oriental melon (Cucumis melo var. makuwa) in Asia is often limited by two potyviruses, the watermelon infecting type of Papaya ringspot virus (PRSV W) and Zucchini yellow mosaic virus (ZYMV). In order to engineer transgenic resistance to these two viruses, an untranslatable chimeric DNA comprising partial coat protein (CP) sequences of ZYMV and PRSV W was constructed and used to transform the elite cultivar of oriental melon, Silver Light, by Agrobacterium. Greenhouse evaluation by mechanical challenges with ZYMV and PRSV W, alone or together, identified transgenic lines exhibiting different levels of resistance or complete immunity to ZYMV and PRSV W. Molecular analyses of transgenic lines revealed random insertion of transgene into the host genome, with insert numbers differing among transformants. There was no correlation between transgene insert numbers and the degree of resistance expressed by transgenic lines. The levels of accumulation of transgene transcript varied among transgenic lines. However, an inverse correlation was observed between the level of accumulation of transgene transcripts and the degree of virus resistance. Moreover, small interfering (si)RNA was readily detected from the immune and highly resistant lines, but not from the weakly resistant and susceptible lines. Altogether, our results indicated that RNA-mediated post-transcriptional gene silencing (PTGS) was the underlying mechanism of double-virus resistance of the transgenic melon lines. The segregation analysis of the R1 progeny of the immune line ZW-1 indicated that the single inserted transgene is associated with the resistance phenotype and is inherited as a dominant trait. These transgenic melon lines with high degrees of resistance to ZYMV and PRSV W have great potential for the control of ZYMV and PRSV W in C. melo in Asia and elsewhere.

scholarly journals Transgenic Resistance to PVYO Associated with Post-Transcriptional Silencing of P1 Transgene Is Overcome by PVYN Strains That Carry Highly Homologous P1 Sequences and Recover Transgene Expression at Infection

2000 ◽  
Vol 13 (4) ◽  
pp. 366-373 ◽  
Author(s):  
Tuula Mäki-Valkama ◽  
Jari P. T. Valkonen ◽  
Jan F. Kreuze ◽  
Eija Pehu
Keyword(s):  
Transgene Expression ◽  
Potato Virus Y ◽  
Transcriptional Gene Silencing ◽  
Gene Sequences ◽  
Strain Specificity ◽  
Antisense Orientation ◽  
Post Transcriptional Gene Silencing ◽  
Resistance Specificity ◽  
Transgenic Lines

Resistance to Potato virus Y (PVY) has been obtained in our previous studies through expression of the PVY P1 gene in sense or antisense orientation in potato cv. Pito. In the present study, the mechanism and strain specificity of the resistance were analyzed. Several features including low steady-state P1 mRNA expression in the resistant P1 plants indicated that resistance was based on post-transcriptional gene silencing (PTGS). Resistance was specific to PVYO isolates, the PVY strain group from which the P1 transgene was derived. However, according to group analyses, there was no distinguishing characteristic between the PVYO and PVYN strains P1 gene sequences. Therefore, the ability of the PVYN strains to overcome resistance could not be explained solely based on their P1 gene sequences. Infection with PVYN of the PVYO-resistant transgenic lines led to a recovery of expression of the P1 transgene. These data suggested that factors other than sequence homology are required in determination of the resistance specificity.

Situación económica y social de la población de San José del Carmen, San Cristóbal de las Casas, Chiapas

2019 ◽  
Vol 12 (4) ◽  
Author(s):  
Pedro Cadena-Iñiguez ◽  
Eileen Salinas-Cruz ◽  
Jesús Martínez-Sánchez ◽  
Mariano Morales-Guerra ◽  
Romualdo Vásquez-Ortiz ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Theobroma Cacao ◽  
Zea Mays L ◽  
Solanum Tuberosum L ◽  
San Jose ◽  
Phaseolus Vulgaris L ◽  
Las Casas ◽  
Vicia Faba L ◽  
Las Mujeres ◽  
Theobroma Cacao L

Objetivo: Establecer una línea base como fundamento para la intervención e inducción de innovaciones a través de escuelas de campo y planes de negocios. Diseño/metodología/aproximación: El estudio se desarrolló en San José del Carmen, San Cristóbal de Las Casas, Chiapas, México. Se elaboró una encuesta como instrumento para recabar en campo información de familias de la localidad con la finalidad de conocer su situación actual. La localidad de trabajo se eligió utilizando como criterio las poblaciones que se encuentran dentro de Cruzada Nacional contra el hambre y en la clasificación de la pobreza extrema Resultados: La localidad de San José del Carmen es considerada como de alta marginación, la población es bilingüe, su principal lengua es el Tzotzil, el nivel de estudios promedio es hasta el tercer año de primaria, las principales actividades productivas son la siembra de maíz (Zea mays L.), frijol (Phaseolus vulgaris L.), papa (Solanum tuberosum L.) y haba (Vicia faba L.) que son utilizadas principalmente para autoconsumo.  No cuentan con agua potable ya que su sistema es a través de la recaudación de agua de lluvia o a través de un jagüey. Sus principales actividades económicas son la albañilería por parte de los hombres, y venta de productos, tales como el pozol (bebida energética a base de maíz y cacao (Theobroma cacao L.), tostadas y algunos vegetales por parte de las mujeres. Las tostadas son producidas en forma artesanal con un proceso de doble nixtamalización y vendida en bolsas de 20 tostadas cada una, a un costo de MX$10.00 en los mercados de San Cristóbal de las Casas, una a dos veces por semana alrededor de 40 bolsas por día de venta. Limitaciones del estudio/implicaciones: La afiliación zapatista de alguno de los pobladores y el hecho de que algunos habitantes no hablen español, fueron factores que limitaron desarrollar al 100% el estudio; sin embargo, se considera que se tienen los elementos necesarios para establecer la línea base de la situación de San José del Carmen. Conclusiones: El diagnóstico servirá como una herramienta para establecer una intervención a través de innovaciones y planes de negocios en la comunidad de estudio. Los pobladores cuentan con herramientas necesarias para obtener nuevos conocimientos que ayuden a un desarrollo de su producción. El idioma no deberá de ser una limitante para ello.

ALLELE DIVERSITY FOR ACID VACUOLAR INVERTASE GENE Pain-1 FRAGMENT IN POTATO (Solanum tuberosum L.) VARIETIES AND LINES

2018 ◽  
Vol 53 (1) ◽  
pp. 132-139
Author(s):  
M.A. Slugina ◽  
◽  
E.O. Shmelkova ◽  
A.A. Meleshin ◽  
E.Z. Kochieva ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Vacuolar Invertase ◽  
Invertase Gene
Sign in / Sign up

Export Citation Format

Share Document