Occurrence of Coelomomyces indicus, a fungal pathogen of malaria mosquito vector, Anopheles gambiae complex along the Kenya coast

1985 ◽  
Vol 6 (2) ◽  
pp. 199-204 ◽  
Author(s):  
W. A. Otieno ◽  
M. O. Odindo ◽  
D. M. Sabwa
Keyword(s):  
Anopheles Gambiae ◽  
Fungal Pathogen ◽  
Biocontrol Agent ◽  
Mosquito Vector ◽  
Malaria Mosquito ◽  
Laboratory Studies ◽  
Anopheles Gambiae Complex ◽  
Coelomomyces Indicus ◽  
Kenyan Coast ◽  
Thoracic And Abdominal

AbstractA survey of the natural enemies of the main malaria vector in Kenya, Anopheles gambiae Giles, revealed the occurrence of a fungal pathogen in field populations of mosquito larvae. Light and scanning electron microscopy was used in the examination of sporangia of the fungus in determining its identity as Coelomomyces indicus Iyengar (Order: Blastocladiales; Family: Coelomomycetaceae). The resting sporangia were found in the thoracic and abdominal cavities of the larvae. A mortality of 63% or higher was found to occur among the larvae of A. gambiae in a two season observation along the Kenyan coast. The authors stress the need for more intensified ecological and laboratory studies aimed at developing C. indicus as a biocontrol agent.

scholarly journals A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae

2016 ◽  
Vol 34 (1) ◽  
pp. 78-83 ◽  
Author(s):  
Andrew Hammond ◽  
Roberto Galizi ◽  
Kyros Kyrou ◽  
Alekos Simoni ◽  
Carla Siniscalchi ◽  
...  
Keyword(s):  
Anopheles Gambiae ◽  
Drive System ◽  
Mosquito Vector ◽  
Gene Drive ◽  
Malaria Mosquito ◽  
Female Reproduction

scholarly journals Midgut Microbiota of the Malaria Mosquito Vector Anopheles gambiae and Interactions with Plasmodium falciparum Infection

2012 ◽  
Vol 8 (5) ◽  
pp. e1002742 ◽  
Author(s):  
Anne Boissière ◽  
Majoline T. Tchioffo ◽  
Dipankar Bachar ◽  
Luc Abate ◽  
Alexandra Marie ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Anopheles Gambiae ◽  
Falciparum Infection ◽  
Mosquito Vector ◽  
Malaria Mosquito ◽  
Plasmodium Falciparum Infection

scholarly journals The regulatory genome of the malaria vector Anopheles gambiae: integrating chromatin accessibility and gene expression

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
José L Ruiz ◽  
Lisa C Ranford-Cartwright ◽  
Elena Gómez-Díaz
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Anopheles Gambiae ◽  
Mosquito Control ◽  
Regulatory Elements ◽  
Chromatin Accessibility ◽  
Malaria Vectors ◽  
Specific Gene ◽  
Mosquito Vector ◽  
Human Malaria

Abstract Anopheles gambiae mosquitoes are primary human malaria vectors, but we know very little about their mechanisms of transcriptional regulation. We profiled chromatin accessibility by the assay for transposase-accessible chromatin by sequencing (ATAC-seq) in laboratory-reared A. gambiae mosquitoes experimentally infected with the human malaria parasite Plasmodium falciparum. By integrating ATAC-seq, RNA-seq and ChIP-seq data, we showed a positive correlation between accessibility at promoters and introns, gene expression and active histone marks. By comparing expression and chromatin structure patterns in different tissues, we were able to infer cis-regulatory elements controlling tissue-specific gene expression and to predict the in vivo binding sites of relevant transcription factors. The ATAC-seq assay also allowed the precise mapping of active regulatory regions, including novel transcription start sites and enhancers that were annotated to mosquito immune-related genes. Not only is this study important for advancing our understanding of mechanisms of transcriptional regulation in the mosquito vector of human malaria, but the information we produced also has great potential for developing new mosquito-control and anti-malaria strategies.

scholarly journals Low-resolution genome map of the malaria mosquito Anopheles gambiae.

1991 ◽  
Vol 88 (24) ◽  
pp. 11187-11191 ◽  
Author(s):  
L. B. Zheng ◽  
R. D. Saunders ◽  
D. Fortini ◽  
A. della Torre ◽  
M. Coluzzi ◽  
...  
Keyword(s):  
Anopheles Gambiae ◽  
Malaria Mosquito ◽  
Low Resolution ◽  
Genome Map ◽  
Mosquito Anopheles Gambiae

scholarly journals Odorant reception in the malaria mosquito Anopheles gambiae

Nature ◽  
2010 ◽  
Vol 464 (7285) ◽  
pp. 66-71 ◽  
Author(s):  
Allison F. Carey ◽  
Guirong Wang ◽  
Chih-Ying Su ◽  
Laurence J. Zwiebel ◽  
John R. Carlson
Keyword(s):  
Anopheles Gambiae ◽  
Malaria Mosquito ◽  
Mosquito Anopheles Gambiae

scholarly journals Bivalent Carbamates as Novel Control Agents of the Malaria Mosquito, Anopheles gambiae

2016 ◽  
Vol 70 (10) ◽  
pp. 704-708 ◽  
Author(s):  
James M. Mutunga ◽  
Qiao-Hong Chen ◽  
Dawn M. Wong ◽  
Polo C-H. Lam ◽  
Jianyong Li ◽  
...  
Keyword(s):  
Anopheles Gambiae ◽  
Malaria Mosquito

scholarly journals Transgenic Expression of the Anti-parasitic Factor TEP1 in the Malaria Mosquito Anopheles gambiae

2017 ◽  
Vol 13 (1) ◽  
pp. e1006113 ◽  
Author(s):  
Gloria Volohonsky ◽  
Ann-Katrin Hopp ◽  
Mélanie Saenger ◽  
Julien Soichot ◽  
Heidi Scholze ◽  
...  
Keyword(s):  
Anopheles Gambiae ◽  
Malaria Mosquito ◽  
Transgenic Expression ◽  
Mosquito Anopheles Gambiae

A GacS deficiency does not affectPseudomonas chlororaphisPA23 fitness when growing on canola, in aged batch culture or as a biofilm

2006 ◽  
Vol 52 (12) ◽  
pp. 1177-1188 ◽  
Author(s):  
N Poritsanos ◽  
C Selin ◽  
W G.D Fernando ◽  
S Nakkeeran ◽  
T.R. de Kievit
Keyword(s):  
Antifungal Activity ◽  
Batch Culture ◽  
Hydrogen Cyanide ◽  
Fungal Pathogen ◽  
Biocontrol Agent ◽  
Antifungal Compounds ◽  
Pseudomonas Chlororaphis ◽  
Wild Type ◽  
Biocontrol Activity ◽  
Competition Assays

Pseudomonas chlororaphis PA23 is a biocontrol agent that protects against the fungal pathogen Sclerotinia sclerotiorum. Employing transposon mutagenesis, we isolated a gacS mutant that no longer exhibited antifungal activity. Pseudomonas chlororaphis PA23 was previously reported to produce the nonvolatile antibiotics phenazine 1-carboxylic acid and 2-hydroxyphenazine. We report here that PA23 produces additional compounds, including protease, lipase, hydrogen cyanide, and siderophores, that may contribute to its biocontrol ability. In the gacS mutant background, generation of these products was markedly reduced or delayed with the exception of siderophores, which were elevated. Not surprisingly, this mutant was unable to protect canola from disease incited by S. sclerotiorum. The gacS mutant was able to sustain itself in the canola phyllosphere, therefore, the loss of biocontrol activity can be attributed to a reduced production of antifungal compounds and not a declining population size. Competition assays between the mutant and wild type revealed equivalent fitness in aged batch culture; consequently, the gacS mutation did not impart a growth advantage in the stationary phase phenotype. Under minimal nutrient conditions, the gacS-deficient strain produced a tenfold less biofilm than the wild type. However, no difference was observed in the ability of the mutant biofilm to protect cells from lethal antibiotic challenge.Key words: Pseudomonas, biocontrol, gacS, fitness, biofilms.

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