Optimization in sample stacking for high-performance capillary electrophoresis

1991 ◽  
Vol 63 (18) ◽  
pp. 2042-2047 ◽  
Author(s):  
Dean S. Burgi ◽  
Ring Ling. Chien
Keyword(s):  
Capillary Electrophoresis ◽  
High Performance ◽  
Sample Stacking ◽  
High Performance Capillary Electrophoresis

High-performance capillary electrophoresis of proteins from the fluid lining of the lungs of rats exposed to perfluoroisobutylene

1991 ◽  
Vol 559 (1-2) ◽  
pp. 411-429 ◽  
Author(s):  
L.R. Gurley ◽  
J.S. Buchanan ◽  
J.E. London ◽  
D.M. Stavert ◽  
B.E. Lehnert
Keyword(s):  
Capillary Electrophoresis ◽  
High Performance ◽  
High Performance Capillary Electrophoresis ◽  
Electrophoresis Of Proteins

Preparation and characterization of a stable polyacrylamide sieving matrix-filled capillary for high-performance capillary electrophoresis

1994 ◽  
Vol 661 (1-2) ◽  
pp. 315-321 ◽  
Author(s):  
Manabu Nakatani ◽  
Akimasa Skibukawa ◽  
Terumichi Nakagawa
Keyword(s):  
Capillary Electrophoresis ◽  
High Performance ◽  
High Performance Capillary Electrophoresis

High-performance capillary electrophoresis of SDS-protein complexes using UV-transparent polymer networks

1992 ◽  
Vol 64 (22) ◽  
pp. 2665-2671 ◽  
Author(s):  
Katalin. Ganzler ◽  
K. S. Greve ◽  
A. S. Cohen ◽  
B. L. Karger ◽  
Andras. Guttman ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
High Performance ◽  
Protein Complexes ◽  
Polymer Networks ◽  
High Performance Capillary Electrophoresis

Separation of Small Molecules by High-Performance Capillary Electrophoresis

1992 ◽  
pp. 301-341 ◽  
Author(s):  
Charles W. Demarest ◽  
Elizabeth A. Monnot-Chase ◽  
James Jiu ◽  
Robert Weinberger
Keyword(s):  
Capillary Electrophoresis ◽  
Small Molecules ◽  
High Performance ◽  
High Performance Capillary Electrophoresis

scholarly journals SSR Marker-Assisted Management of Parental Germplasm in Sugarcane (Saccharum spp. hybrids) Breeding Programs

Agronomy ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 449 ◽  
Author(s):  
Jiantao Wu ◽  
Qinnan Wang ◽  
Jing Xie ◽  
Yong-Bao Pan ◽  
Feng Zhou ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Capillary Electrophoresis ◽  
Population Structure ◽  
Ssr Markers ◽  
High Performance ◽  
Breeding Programs ◽  
Saccharum Spp ◽  
Parental Lines ◽  
Assisted Management ◽  
High Performance Capillary Electrophoresis

Sugarcane (Saccharum spp. hybrids) is an important sugar and bioenergy crop with a high aneuploidy, complex genomes and extreme heterozygosity. A good understanding of genetic diversity and population structure among sugarcane parental lines is a prerequisite for sugarcane improvement through breeding. In order to understand genetic characteristics of parental lines used in sugarcane breeding programs in China, 150 of the most popular accessions were analyzed with 21 fluorescence-labeled simple sequence repeats (SSR) markers and high-performance capillary electrophoresis (HPCE). A total of 226 SSR alleles of high-resolution capacity were identified. Among the series obtained from different origins, the YC-series, which contained eight unique alleles, had the highest genetic diversity. Based on the population structure analysis, the principal coordinate analysis (PCoA) and phylogenetic analysis, the 150 accessions were clustered into two distinct sub-populations (Pop1 and Pop2). Pop1 contained the majority of clones introduced to China (including 28/29 CP-series accessions) while accessions native to China clustered in Pop2. The analysis of molecular variance (AMOVA), fixation index (Fst) value and gene flow (Nm) value all indicated the very low genetic differentiation between the two groups. This study illustrated that fluorescence-labeled SSR markers combined with high-performance capillary electrophoresis (HPCE) could be a very useful tool for genotyping of the polyploidy sugarcane. The results provided valuable information for sugarcane breeders to better manage the parental germplasm, choose the best parents to cross, and produce the best progeny to evaluate and select for new cultivar(s).

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