Regioselective electrode system with a poly(perfluoro sulfonic acid)-coated electrode based on cyclodextrin complexation

1986 ◽  
Vol 58 (9) ◽  
pp. 2096-2097 ◽  
Author(s):  
Tomokazu. Matsue ◽  
Uichi. Akiba ◽  
Tetsuo. Osa
2011 ◽  
Vol 298 ◽  
pp. 56-62 ◽  
Author(s):  
Mei Ma ◽  
Xiao Ping Fan ◽  
Zhao Dai ◽  
Xiao Qing Wang ◽  
Qing Yin Zhang

A novel DNA biosensor based on layer-by-layer self-assembled multi-walled carbon nanotubes (MWNTs) functionalized with a mercapto group (SH-MWNTs) and gold nano-particles (GNPs) was presented, where anthraquinone-2-sulfonic acid sodium salt (AQMS) was used as hybridization indicator. The differential pulse voltammetry responses demonstrated that this DNA/GNPs/SH-MWCNTs/Au biosensor was enabled to specifically detect the single-base mismatch DNA sequence in phosphate buffer solution with pH 7.4 containing 0.3 mol/L Na+ and 1.0 mmol/L AQMS. The result showed that when the target DNA concentration was 1.0×10-10 to 1.6×10-5 mol/L, the cathodic peak current of Au electrode system with AQMS as indicator was linearly related to complementary NDA concentration, and the detection limit was about 3.82×10-11 mol/L and had good stability and specificity.


Author(s):  
E. S. Boatman ◽  
G. E. Kenny

Information concerning the morphology and replication of organism of the family Mycoplasmataceae remains, despite over 70 years of study, highly controversial. Due to their small size observations by light microscopy have not been rewarding. Furthermore, not only are these organisms extremely pleomorphic but their morphology also changes according to growth phase. This study deals with the morphological aspects of M. pneumoniae strain 3546 in relation to growth, interaction with HeLa cells and possible mechanisms of replication.The organisms were grown aerobically at 37°C in a soy peptone yeast dialysate medium supplemented with 12% gamma-globulin free horse serum. The medium was buffered at pH 7.3 with TES [N-tris (hyroxymethyl) methyl-2-aminoethane sulfonic acid] at 10mM concentration. The inoculum, an actively growing culture, was filtered through a 0.5 μm polycarbonate “nuclepore” filter to prevent transfer of all but the smallest aggregates. Growth was assessed at specific periods by colony counts and 800 ml samples of organisms were fixed in situ with 2.5% glutaraldehyde for 3 hrs. at 4°C. Washed cells for sectioning were post-fixed in 0.8% OSO4 in veronal-acetate buffer pH 6.1 for 1 hr. at 21°C. HeLa cells were infected with a filtered inoculum of M. pneumoniae and incubated for 9 days in Leighton tubes with coverslips. The cells were then removed and processed for electron microscopy.


2020 ◽  
Author(s):  
Adam Bruce Ung ◽  
G. K. Surya Prakash ◽  
Thieo E. Hogen-Esch

2020 ◽  
Author(s):  
Adam Bruce Ung ◽  
G. K. Surya Prakash ◽  
Thieo E. Hogen-Esch ◽  
Adam Bruce Ung

2009 ◽  
Author(s):  
James C. Christensen ◽  
Justin R. Estepp ◽  
Glenn F. Wilson ◽  
Iris M. Davis
Keyword(s):  

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