Verification of an algorithm for actin tail detection of subviral particles by comparison with expert validated data

The locomotion of subviral particles of Marburg virus has been shown to be primarily actin based. For this work, a virologist selected 14 subviral particles that show actin tails in fluorescence image sequences. Using the tracked coordinates, examination areas around these subviral particles are defined. The brightness of within the examination area behind the subviral particle is analysed. In addition, the speed of the particle in each frame is calculated to investigate potential correlations between actin activity and particle speed. The results show that actin tracks can be automatically detected and analysed. First hints of a correlation between subviral particle movement and actin activity could be gathered with the presented actin tail quantifier.

Evaporation and deposition of inclined colloidal droplets

Colloidal droplets on flat solid substrates commonly leave symmetric ring-like deposits due to coffee-ring flows during evaporation. On inclined substrates, droplet shapes may become asymmetric by gravity. On this basis, it is not clear how their evaporation dynamics and final deposits are changed depending on inclination. Here we explore evaporation and deposition dynamics of colloidal droplets on inclined substrates, mainly by controlling colloidal particle size, substrate inclination, and relative humidity, which are crucial to gravitational intervention and evaporation dynamics. We experimentally investigate two different flows with opposite directions: downward sedimentation flows by gravity ($$v_s$$ v s ) and upward capillary flows by evaporation ($$v_c$$ v c ). We find that the competition of two flows determines the formation of final deposits with a flow speed ratio of $$\alpha = v_s/v_c$$ α = v s / v c . Notably, for $$\alpha$$ α $$\ll$$ ≪ 1, evaporation-driven upward flows overwhelm sedimentation-driven downward flows, resulting in accentuated particle movement towards the top ring, which seems to defy gravitational intervention. We suggest a possible explanation for the flow speed dependence of final deposits in evaporating colloidal droplets. This study offers a framework to understand the intervention of inclination to the formation of final deposits and how to overcome the deposit pattern radial asymmetry, achieving symmetric deposit widths from inclined colloidal droplets.

Evaluating Gyratory Compaction Characteristics of Unbound Permeable Aggregate Base Materials from Meso-Scale Particle Movement Measured by Smart Sensing Technology

The quality of compaction of unbound aggregate materials with permeable gradation plays a vital role in their field performance; however, there are currently few unanimously accepted techniques or quality control criteria available for ensuring adequate compaction of such materials in either laboratory or field applications. This paper presented testing results of a laboratory gyratory compaction study where the combinations of gyratory parameters were properly designed using the orthogonal array theory. Innovative real-time particle motion sensors were employed to record particle movement characteristics during the compaction process and provide a meso-scale explanation about compaction mechanisms. Particle abrasion and breakage were also quantified from particle shape digitized from the three-dimensional (3D) laser scanner before and after compaction. The optimal combination of gyratory parameters that yields the best compaction performance was determined from the orthogonal testing results with the relative importance of major influencing parameters ranked accordingly. Meso-scale particle movement at the upper center and center side positions of the specimen are promising indicators of compaction quality. The gyratory compaction process can be consistently divided into three distinct stages according to both macro-scale performance indicators and meso-scale particle movement characteristics. A statistically significant bi-linear relationship was found to exist between relative breakage index and maximum abrasion depth, whereas the quality of compaction and the extent of particle breakage appear to be positively correlated, thus necessitating the cost-effective balance between them. The results of this study could provide technical insights and guidance to field compaction of unbound permeable aggregates.

The role of thermal diffusion, particle clusters, hydrodynamic and magnetic forces on the flow behaviour of magneto-polymer composites

In this paper, we study the shear-induced flow of magneto-polymer composites, consisting of dispersions of magnetic particles in solutions of polymers, as a competition between the colloidal forces amid particles and their bulk transport induced by the hydrodynamic forces. For this aim, we analyse the role of different experimental parameters. Firstly, by using only solutions of a well-known anionic polymer (sodium alginate), we provoke a moderate hindering of particle movement, but keeping the liquid-like state of the samples. On the contrary, a gel-like behaviour is conferred to the samples when a cationic polymer (chitosan) is additionally added, which further reduces the particle movement. We analyse the effect of an applied magnetic field, which is opposed to particle transport by hydrodynamic forces, by inducing magnetic attraction between the particles. We perform the analysis under both stationary and oscillatory shear. We show that by using dimensionless numbers the differences between samples and experimental conditions are emphasized. In all cases, as expected, the transport of particles driven by bulk hydrodynamic forces dominates at high values of the shear rate. This article is part of the theme issue ‘Transport phenomena in complex systems (part 1)’.

Pattern interaction effect

Unexpected responses of physical systems to external stimuli can be observed when the stimuli are organized into spatial patterns and, especially, when stimuli of different physical origins are involved, leading to the pattern interaction problem. Combinations of weak stimuli—individually only capable of producing marginal local responses—can produce a global response without involving any bifurcations. Its existence is demonstrated by the interaction of properly tuned topography and temperature patterns. When these patterns overlap in a symmetry preserving manner, the resulting convection has the form of local rolls. When these patterns are misaligned, the resulting convection involves global horizontal particle movement with direction depending on the type of misalignment.

Study on the Relationship between Wear and Flow Characteristics of a Centrifugal Pump at Different Mass Concentrations

In order to study the wear characteristic of a centrifugal pump at different mass concentrations, simulation and experimental research were carried out. The simulation was based on the DPM (discrete phase model) to complete the coupling of particles and the flow field. The experimental research included a performance test and a wear test. Through the comparison of the simulation and experimental research results, the relationship between the particle movement and the wear was analyzed, and the flow field was analyzed through the energy gradient theory. The energy gradient and the particle movement were combined to explain the wear characteristics. When the particles entered the impeller flow area, they directly hit the leading edge of the blade and the hub wall. The particles were sinking due to the flow field, which caused the particles to accumulate near the hub and the pressure surface. These places were at the most severely worn wall. The farther away from the axis the position was, the greater the relative velocity difference between the particles and the wall was, so that wear occurred first in these places. The low-energy properties near the hub made particles gather there, which was also the most serious cause of hub wear.