Quantitation of Underivatized Free Amino Acids in Mammalian Cell Culture Media Using Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

2003 ◽  
Vol 75 (19) ◽  
pp. 5046-5053 ◽  
Author(s):  
Jennifer E. Dally ◽  
Joselina Gorniak ◽  
Rita Bowie ◽  
Catherine M. Bentzley
Keyword(s):  
Free Amino ◽  
Culture Media ◽  
Mammalian Cell Culture ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Cell Culture Media ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Influence of Culture Media on Detection of Carbapenem Hydrolysis by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

2016 ◽  
Vol 54 (7) ◽  
pp. 1896-1898 ◽  
Author(s):  
Ana Carolina Ramos ◽  
Cecília Godoy Carvalhaes ◽  
Jhonatha Rodrigo Cordeiro-Moura ◽  
Anna Carolina Rockstroh ◽  
Antonia Maria Oliveira Machado ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Culture Media ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

In this study, we evaluated the influence of distinct bacterial growth media on detection of carbapenemase hydrolysis by matrix-assisted laser desorption ionization–time of flight mass spectrometry. False-negative results were observed for OXA-25-, OXA-26-, and OXA-72-producingAcinetobacter baumanniiisolates grown on MacConkey agar medium. The other culture media showed 100% sensitivity and 100% specificity for detecting carbapenemase.

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