High-Throughput Single Copy DNA Amplification and Cell Analysis in Engineered Nanoliter Droplets

2008 ◽  
Vol 80 (10) ◽  
pp. 3522-3529 ◽  
Author(s):  
Palani Kumaresan ◽  
Chaoyong James Yang ◽  
Samantha A. Cronier ◽  
Robert G. Blazej ◽  
Richard A. Mathies
Keyword(s):  
High Throughput ◽  
Dna Amplification ◽  
Single Copy ◽  
Cell Analysis ◽  
Copy Dna ◽  
Single Copy Dna

Human XX males with Y single-copy DNA fragments

Nature ◽  
1984 ◽  
Vol 307 (5947) ◽  
pp. 172-173 ◽  
Author(s):  
Georges Guellaen ◽  
Myriam Casanova ◽  
Colin Bishop ◽  
Danielle Geldwerth ◽  
Gabriel Andre ◽  
...  
Keyword(s):  
Single Copy ◽  
Dna Fragments ◽  
Xx Males ◽  
Copy Dna ◽  
Single Copy Dna

scholarly journals Physical map of the Saccharomyces cerevisiae genome at 110-kilobase resolution.

Genetics ◽  
1991 ◽  
Vol 127 (4) ◽  
pp. 681-698 ◽  
Author(s):  
A J Link ◽  
M V Olson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Hybridization ◽  
Physical Map ◽  
Single Copy ◽  
Restriction Endonucleases ◽  
Yeast Genome ◽  
Hybridization Probes ◽  
Copy Dna ◽  
Single Copy Dna

Abstract A physical map of the Saccharomyces cerevisiae genome is presented. It was derived by mapping the sites for two restriction endonucleases, SfiI and NotI, each of which recognizes an 8-bp sequence. DNA-DNA hybridization probes for genetically mapped genes and probes that span particular SfiI and NotI sites were used to construct a map that contains 131 physical landmarks--32 chromosome ends, 61 SfiI sites and 38 NotI sites. These landmarks are distributed throughout the non-rDNA component of the yeast genome, which comprises 12.5 Mbp of DNA. The physical map suggests that those genes that can be detected and mapped by standard genetic methods are distributed rather uniformly over the full physical extent of the yeast genome. The map has immediate applications to the mapping of genes for which single-copy DNA-DNA hybridization probes are available.

Tobacco single-copy DNA is highly homologous to sequences present in the genomes of its diploid progenitors

1985 ◽  
Vol 198 (2) ◽  
pp. 290-298 ◽  
Author(s):  
Jack K. Okamuro ◽  
Robert B. Goldberg
Keyword(s):  
Single Copy ◽  
Copy Dna ◽  
Single Copy Dna

Electron microscopy of Achlya deoxyribonucleic acid sequence organization

1981 ◽  
Vol 1 (2) ◽  
pp. 136-143
Author(s):  
M Pellegrini ◽  
W E Timberlake ◽  
R B Goldberg
Keyword(s):  
Dna Sequences ◽  
Deoxyribonucleic Acid ◽  
Microscopic Analysis ◽  
Single Copy ◽  
Electron Microscopic ◽  
Sequence Organization ◽  
Double Stranded Dna ◽  
Copy Dna ◽  
Gene 32 ◽  
Single Copy Dna

Electron microscopic analysis of reassociated deoxyribonucleic acid (DNA) from the aquatic fungus Achlya bisexualis revealed details of the sequence arrangement of the inverted repeats and both the highly and moderately repetitive sequence clusters. We used the gene 32 protein-ethidium bromide technique for visualizing the DNA molecules, a procedure which provides excellent contrast between single- and double-stranded DNA regions. Long (greater than 6-kilobase) DNA fragments were isolated after reannealing to two different repetitive C0t values, and the renatured structures were then visualized in an electron microscope. Our results showed that the inverted repeat sequences were short (0.5 kilobase, number-average) and separated by nonhomologous DNA of various lengths. These pairs of sequences were not clustered within the genome. Both highly repetitive and moderately repetitive DNA sequences were organized as tandem arrays of precisely paired, regularly repeating units. No permuted clusters of repeating sequences were observed, nor was there evidence of interspersion of repetitive with single-copy DNA sequences in the Achlya genome.

scholarly journals Genomic distribution of repeated and single copy DNA sequences inPenicillium funiculosum

1987 ◽  
Vol 40 (2-3) ◽  
pp. 315-319 ◽  
Author(s):  
N.A. Sahasrabudhe ◽  
M.N. Sainani ◽  
V.S. Gupta ◽  
P.K. Ranjekar
Keyword(s):  
Dna Sequences ◽  
Single Copy ◽  
Genomic Distribution ◽  
Copy Dna ◽  
Single Copy Dna

Single-copy DNA and vertebrate phylogeny

1982 ◽  
Vol 34 (1-2) ◽  
pp. 93-101 ◽  
Author(s):  
A. Morescalchi ◽  
E. Olmo
Keyword(s):  
Single Copy ◽  
Copy Dna ◽  
Single Copy Dna

Analysis of human repeat sequence DNA cotranscribed with single copy DNA from the MCF-7 human breast cancer cell line

1989 ◽  
Vol 17 (2) ◽  
pp. 417-418
Author(s):  
ANDREW MORGAN ◽  
DAVID COLLINS ◽  
FRANK GANNON
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Single Copy ◽  
Repeat Sequence ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Copy Dna ◽  
Single Copy Dna ◽  
Mcf 7
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