Excess Adsorption of Commonly Used Organic Solvents from Water on Nonend-Capped C18-Bonded Phases in Reversed-Phase Liquid Chromatography

2009 ◽  
Vol 81 (15) ◽  
pp. 6334-6346 ◽  
Author(s):  
Szymon Bocian ◽  
Péter Vajda ◽  
Attila Felinger ◽  
Bogusław Buszewski
Keyword(s):  
Liquid Chromatography ◽  
Organic Solvents ◽  
Reversed Phase ◽  
Excess Adsorption ◽  
Reversed Phase Liquid Chromatography ◽  
Bonded Phases ◽  
Phase Liquid

Immunoaffinity clean-up of mycotoxins with organic solvent-free elution

2014 ◽  
Vol 7 (2) ◽  
pp. 115-120 ◽  
Author(s):  
J. Stroka ◽  
J. Seidler
Keyword(s):  
Liquid Chromatography ◽  
Organic Solvent ◽  
Ochratoxin A ◽  
Organic Solvents ◽  
Reversed Phase ◽  
Solvent Free ◽  
Reversed Phase Liquid Chromatography ◽  
Chromatography Column ◽  
Phase Liquid

A novel immunoaffinity (IA) clean-up procedure for the isolation of mycotoxins from maize extracts is described. Commonly, mycotoxins are eluted with organic solvents from IA columns after isolation. The new procedure uses water at 70 °C as alternative. The resulting eluate is suitable for direct and complete injection onto a reversed-phase liquid-chromatography column. Evaporation, reconstitution or dilution are not required. The procedure has been tested for a variety of mycotoxins, including deoxynivalenol (DON) zearalenone, T-2 and HT-2 toxins, aflatoxins and ochratoxin A in an array of different matrices. The example of DON in maize is discussed in more detail and evaluated for repeatability (5%, n=10) and recovery (95%, n=10).

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