Probing the E/K Peptide Coiled-Coil Assembly by Double Electron–Electron Resonance and Circular Dichroism

Biochemistry ◽  
2020 ◽  
Author(s):  
Elena A. Golysheva ◽  
Aimee L. Boyle ◽  
Barbara Biondi ◽  
Paolo Ruzza ◽  
Alexander Kros ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Coiled Coil ◽  
Electron Resonance ◽  
Double Electron ◽  
Double Electron Electron Resonance ◽  
Circular Dichroïsm

Direct Observation of Chain Lengths and Conformations in Oligofluorene Distributions from Controlled Polymerization by Double Electron-Electron Resonance

2019 ◽  
Author(s):  
Dennis Bücker ◽  
Annika Sickinger ◽  
Julian D. Ruiz Perez ◽  
Manuel Oestringer ◽  
Stefan Mecking ◽  
...  
Keyword(s):  
Chain Length ◽  
Length Distribution ◽  
Catalyst System ◽  
Chain Conformation ◽  
Controlled Polymerization ◽  
Chain Length Distribution ◽  
Electron Resonance ◽  
Double Electron ◽  
The Individual ◽  
Double Electron Electron Resonance

Synthetic polymers are mixtures of different length chains, and their chain length and chain conformation is often experimentally characterized by ensemble averages. We demonstrate that Double-Electron-Electron-Resonance (DEER) spectroscopy can reveal the chain length distribution, and chain conformation and flexibility of the individual n-mers in oligo-(9,9-dioctylfluorene) from controlled Suzuki-Miyaura Coupling Polymerization (cSMCP). The required spin-labeled chain ends were introduced efficiently via a TEMPO-substituted initiator and chain terminating agent, respectively, with an in situ catalyst system. Individual precise chain length oligomers as reference materials were obtained by a stepwise approach. Chain length distribution, chain conformation and flexibility can also be accessed within poly(fluorene) nanoparticles.

In-Cell Double Electron–Electron Resonance at Nanomolar Protein Concentrations

2021 ◽  
pp. 3679-3684
Author(s):  
Svetlana Kucher ◽  
Christina Elsner ◽  
Mariya Safonova ◽  
Stefano Maffini ◽  
Enrica Bordignon
Keyword(s):  
Electron Resonance ◽  
Double Electron ◽  
Double Electron Electron Resonance

Distance Determination in Proteins insideXenopus laevisOocytes by Double Electron−Electron Resonance Experiments

2010 ◽  
Vol 132 (24) ◽  
pp. 8228-8229 ◽  
Author(s):  
Ryuji Igarashi ◽  
Tomomi Sakai ◽  
Hideyuki Hara ◽  
Takeshi Tenno ◽  
Toshiaki Tanaka ◽  
...  
Keyword(s):  
Distance Determination ◽  
Electron Resonance ◽  
Double Electron ◽  
Double Electron Electron Resonance

scholarly journals Shigella flexneri Spa15 Crystal Structure Verified in Solution by Double Electron Electron Resonance

2011 ◽  
Vol 405 (2) ◽  
pp. 427-435 ◽  
Author(s):  
James E.D. Lillington ◽  
Janet E. Lovett ◽  
Steven Johnson ◽  
Pietro Roversi ◽  
Christiane R. Timmel ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Shigella Flexneri ◽  
Electron Resonance ◽  
Double Electron ◽  
Double Electron Electron Resonance

scholarly journals Pulsed EPR characterization of HIV-1 protease conformational sampling and inhibitor-induced population shifts

2016 ◽  
Vol 18 (8) ◽  
pp. 5819-5831 ◽  
Author(s):  
Zhanglong Liu ◽  
Thomas M. Casey ◽  
Mandy E. Blackburn ◽  
Xi Huang ◽  
Linh Pham ◽  
...  
Keyword(s):  
Spin Labeling ◽  
Conformational Sampling ◽  
Electron Resonance ◽  
Pulsed Epr ◽  
Double Electron ◽  
Conformational Landscape ◽  
Double Electron Electron Resonance ◽  
Hiv 1

The conformational landscape of HIV-1 protease can be characterized by double electron–electron resonance (DEER) spin-labeling.

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