Combined Effects of Cadmium and UVB Radiation on Sea Urchin Embryos: Skeleton Impairment Parallels p38 MAPK Activation and Stress Genes Overexpression

2015 ◽  
Vol 28 (5) ◽  
pp. 1060-1069 ◽  
Author(s):  
Rosa Bonaventura ◽  
Roberta Russo ◽  
Francesca Zito ◽  
Valeria Matranga
Keyword(s):  
P38 Mapk ◽  
Sea Urchin ◽  
Combined Effects ◽  
Uvb Radiation ◽  
Mapk Activation ◽  
Stress Genes ◽  
Sea Urchin Embryos

scholarly journals p38 MAPK is essential for secondary axis specification and patterning in sea urchin embryos

Development ◽  
2006 ◽  
Vol 133 (1) ◽  
pp. 21-32 ◽  
Author(s):  
C. A. Bradham
Keyword(s):  
P38 Mapk ◽  
Sea Urchin ◽  
Sea Urchin Embryos ◽  
Secondary Axis ◽  
Axis Specification

UVB radiation prevents skeleton growth and stimulates the expression of stress markers in sea urchin embryos

2005 ◽  
Vol 328 (1) ◽  
pp. 150-157 ◽  
Author(s):  
Rosa Bonaventura ◽  
Veronica Poma ◽  
Caterina Costa ◽  
Valeria Matranga
Keyword(s):  
Sea Urchin ◽  
Uvb Radiation ◽  
Sea Urchin Embryos ◽  
Stress Markers

scholarly journals V-type H+ ATPase Activity is Required for Embryonic Dorsal-Ventral Symmetry Breaking

2021 ◽  
Author(s):  
Daphne Schatzberg ◽  
Christopher F. Thomas ◽  
Patrick Reidy ◽  
Sarah E. Hadyniak ◽  
Viktoriya Skidanova ◽  
...  
Keyword(s):  
Symmetry Breaking ◽  
Atpase Activity ◽  
P38 Mapk ◽  
Sea Urchin ◽  
Hypoxia Inducible Factor ◽  
Bmp Signaling ◽  
Voltage Gradient ◽  
Nodal Signaling ◽  
Sea Urchin Embryos

The mechanism for embryonic dorsal-ventral (DV) symmetry breaking is idiosyncratic to the species, then converges on polarized expression of BMP signaling ligands. Here, we show that V-ATPase (VHA) activity is an early requirement for DV symmetry breaking in sea urchin embryos. In these basal deuterostomes, DV specification is mediated by ventral Nodal expression that leads to the establishment of a BMP signaling gradient. Nodal expression occurs downstream from p38 MAPK, which is transiently asymmetrically active. We show that VHA activity is required for DV symmetry breaking upstream from both p38 MAPK and Nodal. We rescue VHA-mediated ventralization by enforcing Nodal signaling asymmetry. We identify a VHA-dependent DV voltage gradient and also find that VHA activity is required for hypoxia inducible factor (HIF) activation. However, neither hyperpolarization nor HIF activation account for the dorsalizing effects of VHA, implicating a third unknown pathway that connects VHA activity to p38 MAPK symmetry breaking.

Glycoprotein synthesis in developing sea urchin embryos

1978 ◽  
Vol 80 (4) ◽  
pp. 833-840 ◽  
Author(s):  
Alfred E. Brown ◽  
H.Bruce Bosmann
Keyword(s):  
Sea Urchin ◽  
Glycoprotein Synthesis ◽  
Sea Urchin Embryos

scholarly journals SYNTHESIS AND STORAGE OF MICROTUBULE PROTEINS BY SEA URCHIN EMBRYOS

1971 ◽  
Vol 50 (2) ◽  
pp. 516-528 ◽  
Author(s):  
Rudolf A. Raff ◽  
Gerald Greenhouse ◽  
Kenneth W. Gross ◽  
Paul R. Gross
Keyword(s):  
Amino Acids ◽  
Sea Urchin ◽  
Messenger Rna ◽  
Binding Activity ◽  
Total Soluble Protein ◽  
Cell Stage ◽  
Lytechinus Pictus ◽  
Sea Urchin Embryos ◽  
Tritiated Amino Acids ◽  
Vinblastine Sulfate

Studies employing colchicine binding, precipitation with vinblastine sulfate, and acrylamide gel electrophoresis confirm earlier proposals that Arbacia punctulata and Lytechinus pictus eggs and embryos contain a store of microtubule proteins. Treatment of 150,000 g supernatants from sea urchin homogenates with vinblastine sulfate precipitates about 5% of the total soluble protein, and 75% of the colchicine-binding activity. Electrophoretic examination of the precipitate reveals two very prominent bands. These have migration rates identical to those of the A and B microtubule proteins of cilia. These proteins can be made radioactive at the 16 cell stage and at hatching by pulse labeling with tritiated amino acids. By labeling for 1 hr with leucine-3H in early cleavage, then culturing embryos in the presence of unlabeled leucine, removal of newly synthesized microtubule proteins from the soluble pool can be demonstrated. Incorporation of labeled amino acids into microtubule proteins is not affected by culturing embryos continuously in 20 µg/ml of actinomycin D. Microtubule proteins appear, therefore, to be synthesized on "maternal" messenger RNA. This provides the first protein encoded by stored or "masked" mRNA in sea urchin embryos to be identified.

scholarly journals cFLIP-L Inhibits p38 MAPK Activation

2003 ◽  
Vol 278 (29) ◽  
pp. 26831-26837 ◽  
Author(s):  
Annette Grambihler ◽  
Hajime Higuchi ◽  
Steven F. Bronk ◽  
Gregory J. Gores
Keyword(s):  
P38 Mapk ◽  
Mapk Activation

scholarly journals p38 MAPK as an essential regulator of dorsal-ventral axis specification and skeletogenesis during sea urchin development: a re-evaluation

Development ◽  
2017 ◽  
Vol 144 (12) ◽  
pp. 2270-2281 ◽  
Author(s):  
Maria Dolores Molina ◽  
Magali Quirin ◽  
Emmanuel Haillot ◽  
Felipe Jimenez ◽  
Aline Chessel ◽  
...  
Keyword(s):  
P38 Mapk ◽  
Sea Urchin ◽  
Axis Specification

scholarly journals Influence of dispersant application on the toxicity to sea urchin embryos of crude and bunker oils representative of prospective oil spill threats in Arctic and Sub-Arctic seas

2021 ◽  
Vol 172 ◽  
pp. 112922
Author(s):  
Laura DeMiguel-Jiménez ◽  
Nestor Etxebarria ◽  
Xabier Lekube ◽  
Urtzi Izagirre ◽  
Ionan Marigómez
Keyword(s):  
Sea Urchin ◽  
Oil Spill ◽  
Arctic Seas ◽  
Sea Urchin Embryos
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