Using Algal Virus Paramecium bursaria Chlorella Virus as a Human Adenovirus Surrogate for Validation of UV Treatment Systems

2020 ◽  
Vol 54 (23) ◽  
pp. 15507-15515
Author(s):  
Zhe Sun ◽  
Jianing Fu ◽  
Xing Li ◽  
Ernest R. Blatchley ◽  
Zhi Zhou
Keyword(s):  
Human Adenovirus ◽  
Paramecium Bursaria ◽  
Uv Treatment ◽  
Algal Virus ◽  
Chlorella Virus

scholarly journals Characterization of a Novelcis-synandtrans-syn-IIPyrimidine Dimer Glycosylase/AP Lyase from a Eukaryotic Algal Virus,Paramecium bursaria chlorellaVirus-1

1998 ◽  
Vol 273 (21) ◽  
pp. 13136-13142 ◽  
Author(s):  
Amanda K. McCullough ◽  
Matthew T. Romberg ◽  
Simon Nyaga ◽  
Yuanfen Wei ◽  
Thomas G. Wood ◽  
...  
Keyword(s):  
Paramecium Bursaria ◽  
Algal Virus ◽  
Ap Lyase

scholarly journals Sequence-specific 1HN, 13C, and 15N backbone resonance assignments of the 34 kDa Paramecium bursaria Chlorella virus 1 (PBCV1) DNA ligase

2009 ◽  
Vol 3 (1) ◽  
pp. 77-80 ◽  
Author(s):  
Andrea Piserchio ◽  
Pravin A. Nair ◽  
Stewart Shuman ◽  
Ranajeet Ghose
Keyword(s):  
Resonance Assignments ◽  
Paramecium Bursaria ◽  
Dna Ligase ◽  
Backbone Resonance Assignments ◽  
Backbone Resonance ◽  
Chlorella Virus

scholarly journals Microarray Analysis of Paramecium bursaria Chlorella Virus 1 Transcription

2009 ◽  
Vol 84 (1) ◽  
pp. 532-542 ◽  
Author(s):  
Giane M. Yanai-Balser ◽  
Garry A. Duncan ◽  
James D. Eudy ◽  
Dong Wang ◽  
Xiao Li ◽  
...  
Keyword(s):  
Life Cycle ◽  
Virus Infection ◽  
Dna Synthesis ◽  
Comprehensive Evaluation ◽  
Paramecium Bursaria ◽  
Chlorella Virus ◽  
Double Stranded Dna ◽  
Unicellular Green Alga ◽  
Protein Encoding ◽  
Encoding Genes

ABSTRACT Paramecium bursaria chlorella virus 1 (PBCV-1), a member of the family Phycodnaviridae, is a large double-stranded DNA, plaque-forming virus that infects the unicellular green alga Chlorella sp. strain NC64A. The 330-kb PBCV-1 genome is predicted to encode 365 proteins and 11 tRNAs. To monitor global transcription during PBCV-1 replication, a microarray containing 50-mer probes to the PBCV-1 365 protein-encoding genes (CDSs) was constructed. Competitive hybridization experiments were conducted by using cDNAs from poly(A)-containing RNAs obtained from cells at seven time points after virus infection. The results led to the following conclusions: (i) the PBCV-1 replication cycle is temporally programmed and regulated; (ii) 360 (99%) of the arrayed PBCV-1 CDSs were expressed at some time in the virus life cycle in the laboratory; (iii) 227 (62%) of the CDSs were expressed before virus DNA synthesis begins; (iv) these 227 CDSs were grouped into two classes: 127 transcripts disappeared prior to initiation of virus DNA synthesis (considered early), and 100 transcripts were still detected after virus DNA synthesis begins (considered early/late); (v) 133 (36%) of the CDSs were expressed after virus DNA synthesis begins (considered late); and (vi) expression of most late CDSs is inhibited by adding the DNA replication inhibitor, aphidicolin, prior to virus infection. This study provides the first comprehensive evaluation of virus gene expression during the PBCV-1 life cycle.

scholarly journals Photosynthetic Shutdown in Chlorella NC64A Associated with the Infection Cycle of Paramecium bursaria Chlorella Virus-1

1995 ◽  
Vol 108 (4) ◽  
pp. 1431-1438 ◽  
Author(s):  
GGR. Seaton ◽  
K. Lee ◽  
J. Rohozinski
Keyword(s):  
Paramecium Bursaria ◽  
Infection Cycle ◽  
Chlorella Virus

scholarly journals SMRT Sequencing of Paramecium Bursaria Chlorella Virus-1 Reveals Diverse Methylation Stability in Adenines Targeted by Restriction Modification Systems

2020 ◽  
Vol 11 ◽  
Author(s):  
Samantha R. Coy ◽  
Eric R. Gann ◽  
Spiridon E. Papoulis ◽  
Michael E. Holder ◽  
Nadim J. Ajami ◽  
...  
Keyword(s):  
Paramecium Bursaria ◽  
Smrt Sequencing ◽  
Chlorella Virus ◽  
Restriction Modification ◽  
Restriction Modification Systems

scholarly journals Cryopreservation of Paramecium bursaria Chlorella Virus-1 during an active infection cycle of its host

PLoS ONE ◽  
2019 ◽  
Vol 14 (3) ◽  
pp. e0211755 ◽  
Author(s):  
Samantha R. Coy ◽  
Alyssa N. Alsante ◽  
James L. Van Etten ◽  
Steven W. Wilhelm
Keyword(s):  
Paramecium Bursaria ◽  
Infection Cycle ◽  
Active Infection ◽  
Chlorella Virus

scholarly journals Cryopreservation of Paramecium bursaria Chlorella Virus-1 during an active infection cycle of its host

2019 ◽  
Author(s):  
Samantha R. Coy ◽  
Alyssa N. Alsante ◽  
James L. Van Etten ◽  
Steven W. Wilhelm
Keyword(s):  
Post Infection ◽  
Laboratory Culture ◽  
Paramecium Bursaria ◽  
Infection Cycle ◽  
Active Infection ◽  
Virion Assembly ◽  
Virus Particles ◽  
Chlorella Virus ◽  
Chlorella Variabilis ◽  
Reliable Mechanism

AbstractBest practices in laboratory culture management often include cryopreservation of microbiota, but this can be challenging with some virus particles. By preserving viral isolates researchers can mitigate genetic drift and laboratory-induced selection, thereby maintaining genetically consistent strains between experiments. To this end, we developed a method to cryopreserve the model, green-alga infecting virus,Paramecium bursaria Chlorella virus 1(PBCV-1). We explored cryotolerance of the infectivity of this virus particle, whereby freezing without cryoprotectants was found to maintain the highest infectivity (~2.5%). We then assessed the cryopreservation potential of PBCV-1 during an active infection cycle in itsChlorella variabilisNC64A host, and found that virus survivorship was highest (69.5 ± 16.5 %) when the infected host is cryopreserved during mid-late stages of infection (i.e., coinciding with virion assembly). The most optimal condition for cryopreservation was observed at 240 minutes post-infection. Overall, utilizing the cell as a vehicle for viral cryopreservation resulted in 24.9 – 30.1 fold increases in PBCV-1 survival based on 95% confidence intervals of frozen virus particles and virus cryopreserved at 240 minutes post-infection. Given that cryoprotectants are often naturally produced by psychrophilic organisms, we suspect that cryopreservation of infected hosts may be a reliable mechanism for virus persistence in non-growth permitting circumstances in the environment, such as ancient permafrosts.

scholarly journals Deep RNA Sequencing Reveals Hidden Features and Dynamics of Early Gene Transcription in Paramecium bursaria Chlorella Virus 1

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e90989 ◽  
Author(s):  
Guillaume Blanc ◽  
Michael Mozar ◽  
Irina V. Agarkova ◽  
James R. Gurnon ◽  
Giane Yanai-Balser ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Gene Transcription ◽  
Early Gene ◽  
Paramecium Bursaria ◽  
Chlorella Virus

scholarly journals Global Analysis of Chlorella variabilis NC64A mRNA Profiles during the Early Phase of Paramecium bursaria Chlorella Virus-1 Infection

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e90988 ◽  
Author(s):  
Janet M. Rowe ◽  
Adrien Jeanniard ◽  
James R. Gurnon ◽  
Yuannan Xia ◽  
David D. Dunigan ◽  
...  
Keyword(s):  
Early Phase ◽  
Global Analysis ◽  
Paramecium Bursaria ◽  
Chlorella Virus ◽  
Chlorella Variabilis

scholarly journals Three-dimensional structure and function of the Paramecium bursaria chlorella virus capsid

2011 ◽  
Vol 108 (36) ◽  
pp. 14837-14842 ◽  
Author(s):  
X. Zhang ◽  
Y. Xiang ◽  
D. D. Dunigan ◽  
T. Klose ◽  
P. R. Chipman ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Structure And Function ◽  
Paramecium Bursaria ◽  
Dimensional Structure ◽  
Virus Capsid ◽  
Three Dimensional Structure ◽  
Chlorella Virus ◽  
And Function
Sign in / Sign up

Export Citation Format

Share Document