scholarly journals Optimizing the Yield of a Pure Enantiomer by Integrating Chiral Simulated Moving Bed Chromatography and Racemization. Part 1: Experiments

2021 ◽  
Author(s):  
Francesca Breveglieri ◽  
Tuvshinjargal Otgonbayar ◽  
Marco Mazzotti
Keyword(s):  
Simulated Moving Bed ◽  
Moving Bed ◽  
Simulated Moving Bed Chromatography ◽  
Pure Enantiomer

Simulated moving bed chromatography under linear conditions

1997 ◽  
Vol 45 (1) ◽  
pp. 109-115 ◽  
Author(s):  
G. Zhong ◽  
T. Yun ◽  
S. Khattabi ◽  
G. Guiochon
Keyword(s):  
Simulated Moving Bed ◽  
Moving Bed ◽  
Simulated Moving Bed Chromatography

scholarly journals The Application of Simulated Moving-Bed Chromatography for the Separation of Ginsenosides in Sanchi Ginseng and American Ginseng

2020 ◽  
Vol 15 (10) ◽  
pp. 1934578X2096467
Author(s):  
Tai Thi Ngoc Dong ◽  
Chih-Hsiung Lin ◽  
Ming-Tsai Liang
Keyword(s):  
Crude Extract ◽  
Large Scale ◽  
Aqueous Ethanol ◽  
American Ginseng ◽  
Simulated Moving Bed ◽  
Moving Bed ◽  
Pharmaceutical Ingredients ◽  
Antisolvent Precipitation ◽  
Simulated Moving Bed Chromatography ◽  
Significant Difference

In this study, the separation of ginsenosides from the crude extract of Sanchi ginseng and American ginseng, obtained by aqueous ethanol extraction, was conducted in a simulated moving bed (SMB) with cleaning in place (CIP) design. Concentrated products with 86.2%, w/w, and 91.5%, w/w, of ginsenosides were obtained by the utilization of CIP-SMB for Sanchi and American ginseng, respectively. This study reveals that CIP-SMB could enrich ginsenosides by removing the retained impurities, which were initially extracted by aqueous ethanol and not removed by either the antisolvent precipitation for Sanchi ginseng or the ethyl acetate extraction for American ginseng. Although the ginsenosides in both ginsengs are similar, the difficulty in applying chromatography to the crude extract differs. A natural crude extract normally contains compounds with significant difference in retention. A traditional SMB with 4 sections will be, consequently, unable to continuously separate the natural products. This study develops methods for pretreating the crude extract and demonstrates the feasibility of continuously separating ginsenosides by using SMB. SMB is an effective technology for large-scale chromatography and can generally resolve solvent and energy consumption problems. This is expected to promote the industrial use of SMB for the production of concentrated ginsenosides as active pharmaceutical ingredients.

Design of Simulated Moving Bed Chromatography for Amino Acid Separations

1998 ◽  
Vol 37 (10) ◽  
pp. 4023-4035 ◽  
Author(s):  
D.-J. Wu ◽  
Y. Xie ◽  
Z. Ma ◽  
N.-H. L. Wang
Keyword(s):  
Amino Acid ◽  
Simulated Moving Bed ◽  
Moving Bed ◽  
Simulated Moving Bed Chromatography

Supercritical Fluid Extraction of Polygonum cuspidatum and Subsequent Isolation by Simulated Moving Bed Chromatography

2013 ◽  
Vol 704 ◽  
pp. 18-30
Author(s):  
Ming Tsai Liang ◽  
Ru Chien Liang ◽  
Shu Qi Yu ◽  
Ri An Yan ◽  
Ku Yuan Liang
Keyword(s):  
Carbon Dioxide ◽  
Crude Extract ◽  
Operating Conditions ◽  
Effect Of Temperature ◽  
Simulated Moving Bed ◽  
Polygonum Cuspidatum ◽  
Two Stage ◽  
Moving Bed ◽  
Second Stage ◽  
Simulated Moving Bed Chromatography

In this work, supercritical carbon dioxide with ethanol as cosolvent was employed to extract bioactive compounds fromPolygonum Cuspidatum. Effect of temperature for 40 and 50 C on the extraction at 35 MPa was examined. It was found that extraction at 50 C resulted in high yield. A two-stage extraction at 50 C and 35 MPa was then designed to investigate effect of cosolvent on the recovery of resveratrol and emodin. The first stage extraction by solely using carbon dioxide can rarely extract resveratrol, but adding ethanol as cosolvent in the second stage significantly increases the extraction of resverarol and emodin. The crude extract obtained in the second stage was very stable against agglomeration and precipitation, because the first stage extraction helped to remove impurities from thePolygonum Cuspidatum. The stable crude extract was subsequently used as the feed for the SMB (simulated moving bed) separation. In this work, experimental validation was completed by solely using single SMB to isolate resveratrol, emodin, and physcion from the crude extract. As conducting the SMB experiments, the Triangle theory was used to help to determine the separable operating conditions. From the discussion of the experimental results, the criterion to obtain the pure resveratrol and physcion, and the concentrated emodin were depicted. Since both the extraction and the purification are conducted at low temperature, the bioactivity of the produced natural products will totally be preserved. The two-stage extraction and subsequent purification in this paper will provide greener and effective alternative for the development of botanical drugs.

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