Efficient Synthesis of Ir-Polyoxometalate Cluster Using a Continuous Flow Apparatus and STM Investigation of Its Coassembly Behavior on HOPG Surface

ABSTRACT A procedure for the continuous flow incubation of isolated adrenal cells is described. In this way the advantages of continuous flow incubations of adrenal tissue are combined with those of isolated adrenal cells. Suspensions of isolated adrenal cells were prepared by a modification of the collagenase method. A sigmoid dose-response curve was obtained when these cells were incubated with ACTH in batch incubations. Under these conditions (in the presence of 1 mU ACTH/ml) the corticosterone production rate remained constant during at least 240 min. This production rate was linearly related to the number of cells. Pre-incubation of the cells during 3 h resulted in an increased response to ACTH. In continuous flow incubations without ACTH the corticosterone production was negligible. With 100 μU ACTH/ml corticosterone production increased sharply after a short lag period. A maximum was reached after 60–75 min followed by a slow decrease. Cells pre-incubated in the continuous flow apparatus had a slightly diminished ACTH response without loss of affinity to ACTH. The continuous flow incubation of isolated adrenal cells offers new possibilities for the dynamic study of steroid biosynthesis in vitro. The method may also be valuable to study processes in a wide variety of other tissues.

Download Full-text

Efficient synthesis of panaxadiol derivatives using continuous-flow microreactor and evaluation of anti-tumor activity

Chinese Chemical Letters ◽

10.1016/j.cclet.2014.11.013 ◽

2015 ◽

Vol 26 (3) ◽

pp. 334-338 ◽

Cited By ~ 9

Author(s):

Yan Wu ◽

Wei-Qi Chen ◽

Yu-Qing Zhao ◽

Hu-Ri Piao

Keyword(s):

Continuous Flow ◽

Efficient Synthesis ◽

Flow Microreactor ◽

Anti Tumor Activity

Download Full-text

Efficient synthesis of lithium rare-earth tetrafluoride nanocrystals via a continuous flow method

Nano Research ◽

10.1007/s12274-020-2938-y ◽

2020 ◽

Vol 13 (10) ◽

pp. 2837-2846 ◽

Cited By ~ 3

Author(s):

Jinsong Sui ◽

Junyu Yan ◽

Kai Wang ◽

Guangsheng Luo

Keyword(s):

Rare Earth ◽

Continuous Flow ◽

Efficient Synthesis ◽

Flow Method ◽

Continuous Flow Method

Download Full-text

Reaction of allylzinc bromides with carbonyl compounds in a continuous flow system. Efficient synthesis of homoallyl alcohols including artemisia alcohol

The Journal of Organic Chemistry ◽

10.1021/jo00865a029 ◽

1976 ◽

Vol 41 (3) ◽

pp. 550-551 ◽

Cited By ~ 22

Author(s):

John F. Ruppert ◽

James D. White

Keyword(s):

Carbonyl Compounds ◽

Continuous Flow ◽

Flow System ◽

Efficient Synthesis ◽

Continuous Flow System

Download Full-text

Subsecond calcium dynamics in ADP- and thrombin-stimulated platelets: a continuous-flow approach using indo-1

Blood ◽

10.1182/blood.v71.6.1539.bloodjournal7161539 ◽

1988 ◽

Vol 71 (6) ◽

pp. 1539-1543

Author(s):

GD Jones ◽

AR Gear

Keyword(s):

Blood Flow ◽

Continuous Flow ◽

Blood Platelets ◽

Adenosine Diphosphate ◽

Cytosolic Calcium ◽

Human Platelets ◽

Calcium Dynamics ◽

Flow Conditions ◽

Shear Rates ◽

Flow Apparatus

The regulation and kinetics (less than 5 seconds) of cytosolic calcium changes ([Ca2+]i) in stimulated blood platelets have been investigated under physiological blood flow conditions. Using a newly-developed continuous-flow approach with indo-1-loaded human platelets, adenosine diphosphate (ADP, 10 mumol/L) and thrombin (5 U/mL) were equally effective in significantly increasing [Ca2+]i by 0.5 seconds. ADP induced a transient [Ca2+]i peak of 1 to 2 mumol/L near 2 seconds, whereas thrombin caused a sustained and larger response. The first phase (less than 2 seconds) was not influenced by a lack of extracellular Ca2+, in contrast to the subsequent [Ca2+]i increase that only reached about 0.7 mumol/L for either ADP or thrombin. The shear rates used in our continuous-flow apparatus were physiological (less than 1,258 sec-1) and only slightly increased the basal [Ca2+]i of 0.1 mumol/L. Platelet aggregation (less than 5 seconds), assessed by single- particle counting, was not altered in platelets loaded with indo-1/AM (2.5 mumol/L).

Download Full-text