scholarly journals Transcription-Factor-Mediated DNA Looping Probed by High-Resolution, Single-Molecule Imaging in Live E. coli Cells

PLoS Biology ◽  
2013 ◽  
Vol 11 (6) ◽  
pp. e1001591 ◽  
Author(s):  
Zach Hensel ◽  
Xiaoli Weng ◽  
Arvin Cesar Lagda ◽  
Jie Xiao
Keyword(s):  
Transcription Factor ◽  
High Resolution ◽  
Single Molecule ◽  
Dna Looping ◽  
Single Molecule Imaging ◽  
E Coli ◽  
Resolution Single

scholarly journals High-Resolution Single-Molecule FRET via DNA eXchange (FRET X)

Nano Letters ◽  
2021 ◽  
Vol 21 (7) ◽  
pp. 3295-3301 ◽  
Author(s):  
Mike Filius ◽  
Sung Hyun Kim ◽  
Ivo Severins ◽  
Chirlmin Joo
Keyword(s):  
High Resolution ◽  
Single Molecule ◽  
Single Molecule Fret ◽  
Resolution Single

scholarly journals Rigid DNA Beams for High-Resolution Single-Molecule Mechanics

2013 ◽  
Vol 125 (30) ◽  
pp. 7920-7925 ◽  
Author(s):  
Emanuel Pfitzner ◽  
Christian Wachauf ◽  
Fabian Kilchherr ◽  
Benjamin Pelz ◽  
William M. Shih ◽  
...  
Keyword(s):  
High Resolution ◽  
Single Molecule ◽  
Resolution Single

scholarly journals High-Resolution Single-Molecule Fluorescence Imaging of Zeolite Aggregates within Real-Life Fluid Catalytic Cracking Particles

2014 ◽  
Vol 54 (6) ◽  
pp. 1836-1840 ◽  
Author(s):  
Zoran Ristanović ◽  
Marleen M. Kerssens ◽  
Alexey V. Kubarev ◽  
Frank C. Hendriks ◽  
Peter Dedecker ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Imaging ◽  
Single Molecule ◽  
Catalytic Cracking ◽  
Real Life ◽  
Fluid Catalytic Cracking ◽  
Single Molecule Fluorescence ◽  
Resolution Single

scholarly journals Single-molecule imaging reveals the interplay between transcription factors, nucleosomes, and transcriptional bursting

2018 ◽  
Author(s):  
Benjamin T. Donovan ◽  
Anh Huynh ◽  
David A. Ball ◽  
Michael G. Poirier ◽  
Daniel R. Larson ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Single Molecule ◽  
Target Genes ◽  
Burst Size ◽  
Yeast Cells ◽  
Single Molecule Imaging ◽  
Transcriptional Bursting

SummaryTranscription factors show rapid and reversible binding to chromatin in living cells, and transcription occurs in sporadic bursts, but how these phenomena are related is unknown. Using a combination of in vitro and in vivo single-molecule imaging approaches, we directly correlated binding of the transcription factor Gal4 with the transcriptional bursting kinetics of the Gal4 target genes GAL3 and GAL10 in living yeast cells. We find that Gal4 dwell times sets the transcriptional burst size. Gal4 dwell time depends on the affinity of the binding site and is reduced by orders of magnitude by nucleosomes. Using a novel imaging platform, we simultaneously tracked transcription factor binding and transcription at one locus, revealing the timing and correlation between Gal4 binding and transcription. Collectively, our data support a model where multiple polymerases initiate during a burst as long as the transcription factor is bound to DNA, and a burst terminates upon transcription factor dissociation.

scholarly journals Single-molecule imaging of CREB transcription factor in human cortical-like neurons

IBRO Reports ◽  
2019 ◽  
Vol 6 ◽  
pp. S216-S217
Author(s):  
Yuri Atsumi ◽  
Noriyuki Sugo ◽  
Ryohei Iwata ◽  
Pierre Vanderhaeghen ◽  
Nobuhiko Yamamoto
Keyword(s):  
Transcription Factor ◽  
Single Molecule ◽  
Single Molecule Imaging ◽  
Creb Transcription Factor

scholarly journals High Resolution Single Molecule Analysis using Nanopore Recording on Microelectrode Cavity Arrays

2011 ◽  
Vol 100 (3) ◽  
pp. 608a
Author(s):  
Gerhard Baaken ◽  
Srujan K. Dondapati ◽  
Norbert Ankri ◽  
Jürgen Rühe ◽  
Jan C. Behrends
Keyword(s):  
High Resolution ◽  
Single Molecule ◽  
Single Molecule Analysis ◽  
Resolution Single
Sign in / Sign up

Export Citation Format

Share Document