Gold Nanobipyramids as Second Near Infrared Optical Coherence Tomography Contrast Agents for in Vivo Multiplexing Studies

Nano Letters ◽  
2019 ◽  
Vol 20 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Peng Si ◽  
Saba Shevidi ◽  
Edwin Yuan ◽  
Ke Yuan ◽  
Ziv Lautman ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
Near Infrared ◽  
Optical Coherence ◽  
Gold Nanobipyramids

scholarly journals Gold Nanobipyramids as Second Near Infrared Optical Coherence Tomography Contrast Agents for Multiplexed In Vivo Lymphangiography

2019 ◽  
Author(s):  
Peng Si ◽  
Saba Shevidi ◽  
Edwin Yuan ◽  
Ke Yuan ◽  
Ziv Lautman ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Lymph Node ◽  
Contrast Agents ◽  
Near Infrared ◽  
Lymphatic Drainage ◽  
Optical Coherence ◽  
Melanoma Tumor ◽  
Contrast Enhanced ◽  
Gold Nanobipyramids

AbstractDeveloping contrast-enhanced optical coherence tomography (OCT) techniques is important for specific imaging of tissue lesions, molecular imaging, cell-tracking, and highly sensitive microangiography and lymphangiography. Multiplexed OCT imaging in the second near infrared (NIR-II) window is highly desirable since it allows simultaneous imaging and tracking of multiple biological events in high resolution with deeper tissue penetration in vivo. Here we demonstrate that gold nanobipyramids can function as OCT multiplexing contrast agents, allowing the visualization of two separate lymphatic flows occurring simultaneously from different drainage basins into the same lymph node in a live mouse. Contrast-enhanced multiplexed lymphangiography of a melanoma tumor in vivo shows that the peritumoral lymphatic drainage upstream of the tumor is unidirectional, with some drainage directly into the tumor, but the lymphatic drainage from the tumor is multi-directional. We also demonstrate real-time tracking of the contrast agents draining from a melanoma tumor specifically to the sentinel lymph node of the tumor and the three-dimensional distribution of the contrast agents in the lymph node.

scholarly journals A Spectral De-mixing Model for Triplex In Vivo Imaging of Optical Coherence Tomography Contrast Agents

2019 ◽  
Author(s):  
Edwin Yuan ◽  
Peng Si ◽  
Saba Shevidi ◽  
Adam de la Zerda
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agent ◽  
Contrast Agents ◽  
In Vivo Imaging ◽  
Spectroscopic Technique ◽  
Optical Coherence ◽  
Single Voxel ◽  
Gold Nanobipyramids

The ability to detect multiple contrast agents simultaneously would greatly enhance Optical Coherence Tomography (OCT) images, providing nuanced biological context to physiological structures. However, previous OCT contrast agent work has been limited to scenarios where only a single contrast agent could be robustly detected within each voxel. We present a novel spectroscopic technique for de-mixing the spectral signal from multiple OCT contrast agents within a single voxel. We validate our technique in vitro and also demonstrate in vivo imaging of three spectrally distinct gold nanobipyramids, trafficking within the lymphatic system of a live mouse. This approach opens the door to a much broader range of pre-clinical and clinical OCT applications where multiplexed labeling is desirable.

scholarly journals Gold Nanoprisms as Optical Coherence Tomography Contrast Agents in the Second Near Infrared Window for Enhanced Angiography in Live Animals

2018 ◽  
Author(s):  
Peng Si ◽  
Edwin Yuan ◽  
Orly Liba ◽  
Yonatan Winetraub ◽  
Siavash Yousefi ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
Near Infrared ◽  
Mouse Skin ◽  
Optical Coherence ◽  
Vascular Networks ◽  
Gold Nanoprisms ◽  
Dynamic Scattering ◽  
Infrared Window

ABSTRACTOptical coherence tomography angiography (OCTA) is an important tool for investigating vascular networks and microcirculation in living tissue. Traditional OCTA detects blood vessels via intravascular dynamic scattering signals derived from the movements of red blood cells (RBCs). However, the low hematocrit and long latency between RBCs in capillaries makes these OCTA signals discontinuous, leading to incomplete mapping of the vascular networks. OCTA imaging of microvascular circulation is particularly challenging in tumors due to the abnormally slow blood flow in angiogenic tumor vessels and strong attenuation of light by tumor tissue. Here we demonstrate in vivo that gold nanoprisms (GNPRs) can be used as OCT contrast agents working in the second near infrared window, significantly enhancing the dynamic scattering signals in microvessels and improving the sensitivity of OCTA in skin tissue and melanoma tumors in live mice. This is the first demonstration that nanoparticle-based OCT contrast agent work in vivo in the second near infrared window, which allows deeper imaging depth by OCT. With GNPRs as contrast agents, the post-injection OCT angiograms showed 41% and 59% more microvasculature than pre-injection angiograms in healthy mouse skin and melanoma tumors, respectively. By enabling better characterization of microvascular circulation in vivo, GNPR-enhanced OCTA could lead to better understanding of vascular functions during pathological conditions, more accurate measurements of therapeutic response, and improved patient prognoses.

scholarly journals Gold Nanoprisms as Optical Coherence Tomography Contrast Agents in the Second Near-Infrared Window for Enhanced Angiography in Live Animals

ACS Nano ◽  
2018 ◽  
Vol 12 (12) ◽  
pp. 11986-11994 ◽  
Author(s):  
Peng Si ◽  
Edwin Yuan ◽  
Orly Liba ◽  
Yonatan Winetraub ◽  
Siavash Yousefi ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
Near Infrared ◽  
Optical Coherence ◽  
Gold Nanoprisms ◽  
Infrared Window

Potential of contrast agents to enhance in vivo confocal microscopy and optical coherence tomography in dermatology: A review

2019 ◽  
Vol 12 (6) ◽  
Author(s):  
Hans C. Ring ◽  
Niels M. Israelsen ◽  
Ole Bang ◽  
Merete Haedersdal ◽  
Mette Mogensen
Keyword(s):  
Optical Coherence Tomography ◽  
Confocal Microscopy ◽  
Contrast Agents ◽  
Optical Coherence ◽  
In Vivo Confocal Microscopy

In vivo imaging of gold nanorod contrast agents using photothermal optical coherence tomography

2013 ◽  
Author(s):  
Jason M. Tucker-Schwartz ◽  
Travis A. Meyer ◽  
Chetan A. Patil ◽  
Craig L. Duvall ◽  
Melissa C. Skala
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
In Vivo Imaging ◽  
Gold Nanorod ◽  
Optical Coherence

scholarly journals In vivo photothermal optical coherence tomography of endogenous and exogenous contrast agents in the eye

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Maryse Lapierre-Landry ◽  
Andrew Y. Gordon ◽  
John S. Penn ◽  
Melissa C. Skala
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
Optical Coherence

scholarly journals A Spectral Demixing Model for Triplex In Vivo Imaging of Optical Coherence Tomography Contrast Agents

ACS Photonics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 893-900
Author(s):  
Edwin Yuan ◽  
Peng Si ◽  
Yonatan Winetraub ◽  
Saba Shevidi ◽  
Adam de la Zerda
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
In Vivo Imaging ◽  
Optical Coherence
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