scholarly journals Engineering a Metathesis-Catalyzing Artificial Metalloenzyme Based on HaloTag

ACS Catalysis ◽  
2021 ◽  
pp. 6343-6347
Author(s):  
Sandro Fischer ◽  
Thomas R. Ward ◽  
Alexandria D. Liang
Keyword(s):  
Artificial Metalloenzyme

scholarly journals Prodrug Activation by Gold Artificial Metalloenzyme‐Catalyzed Synthesis of Phenanthridinium Derivatives via Hydroamination

2021 ◽  
Author(s):  
Tsung‐Che Chang ◽  
Kenward Vong ◽  
Tomoya Yamamoto ◽  
Katsunori Tanaka
Keyword(s):  
Prodrug Activation ◽  
Artificial Metalloenzyme

scholarly journals Cover Picture: A General Method for Artificial Metalloenzyme Formation through Strain-Promoted Azide-Alkyne Cycloaddition (ChemBioChem 2/2014)

ChemBioChem ◽  
2014 ◽  
Vol 15 (2) ◽  
pp. 173-173
Author(s):  
Hao Yang ◽  
Poonam Srivastava ◽  
Chen Zhang ◽  
Jared C. Lewis
Keyword(s):  
Artificial Metalloenzyme ◽  
General Method

Chemogenetic engineering of nitrobindin toward an artificial epoxygenase

2021 ◽  
Author(s):  
Daniel Friedrich Sauer ◽  
Malte Wittwer ◽  
Ulrich Markel ◽  
Alexander Minges ◽  
Markus Spiertz ◽  
...  
Keyword(s):  
Artificial Metalloenzyme ◽  
Powerful Strategy

Chemogenetic engineering of metalloproteins emerges as a powerful strategy to generate proteins that catalyze non-natural reactions or convert non-natural substrates. Here, we report on an artificial metalloenzyme (ArM) based on...

scholarly journals Redox-switchable siderophore anchor enables reversible artificial metalloenzyme assembly

2018 ◽  
Vol 1 (9) ◽  
pp. 680-688 ◽  
Author(s):  
Daniel J. Raines ◽  
Justin E. Clarke ◽  
Elena V. Blagova ◽  
Eleanor J. Dodson ◽  
Keith S. Wilson ◽  
...  
Keyword(s):  
Artificial Metalloenzyme

scholarly journals On-cell catalysis by surface engineering of live cells with an artificial metalloenzyme

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Mathieu Szponarski ◽  
Fabian Schwizer ◽  
Thomas R. Ward ◽  
Karl Gademann
Keyword(s):  
Surface Engineering ◽  
Live Cells ◽  
Artificial Metalloenzyme

scholarly journals Cofactor Binding Dynamics Influence the Catalytic Activity and Selectivity of an Artificial Metalloenzyme

ACS Catalysis ◽  
2020 ◽  
Vol 10 (20) ◽  
pp. 11783-11790
Author(s):  
Lara Villarino ◽  
Shreyans Chordia ◽  
Lur Alonso-Cotchico ◽  
Eswar Reddem ◽  
Zhi Zhou ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Cofactor Binding ◽  
Artificial Metalloenzyme

scholarly journals An artificial metalloenzyme biosensor can detect ethylene gas in fruits and Arabidopsis leaves

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Kenward Vong ◽  
Shohei Eda ◽  
Yasuhiro Kadota ◽  
Igor Nasibullin ◽  
Takanori Wakatake ◽  
...  
Keyword(s):  
Real Time ◽  
Chemical Space ◽  
Ethylene Biosynthesis ◽  
Ruthenium Catalyst ◽  
Design And Development ◽  
Cross Metathesis ◽  
Rapid Changes ◽  
Artificial Metalloenzymes ◽  
Induced Changes ◽  
Artificial Metalloenzyme

AbstractEnzyme biosensors are useful tools that can monitor rapid changes in metabolite levels in real-time. However, current approaches are largely constrained to metabolites within a limited chemical space. With the rising development of artificial metalloenzymes (ArM), a unique opportunity exists to design biosensors from the ground-up for metabolites that are difficult to detect using current technologies. Here we present the design and development of the ArM ethylene probe (AEP), where an albumin scaffold is used to solubilize and protect a quenched ruthenium catalyst. In the presence of the phytohormone ethylene, cross metathesis can occur to produce fluorescence. The probe can be used to detect both exogenous- and endogenous-induced changes to ethylene biosynthesis in fruits and leaves. Overall, this work represents an example of an ArM biosensor, designed specifically for the spatial and temporal detection of a biological metabolite previously not accessible using enzyme biosensors.

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