Histidine-Mediated Intramolecular Electrostatic Repulsion for Controlling pH-Dependent Protein–Protein Interaction

2019 ◽  
Vol 14 (12) ◽  
pp. 2729-2736 ◽  
Author(s):  
Hideki Watanabe ◽  
Chuya Yoshida ◽  
Ayako Ooishi ◽  
Yasuto Nakai ◽  
Momoko Ueda ◽  
...  
Keyword(s):  
Protein Interaction ◽  
Electrostatic Repulsion ◽  
Protein Protein Interaction ◽  
Dependent Protein ◽  
Ph Dependent

Biochemical Characterization of RAR1 Cysteine- and Histidine-Rich Domains (CHORDs):  A Novel Class of Zinc-Dependent Protein−Protein Interaction Modules†

Biochemistry ◽  
2007 ◽  
Vol 46 (6) ◽  
pp. 1612-1623 ◽  
Author(s):  
Charles T. Heise ◽  
Cécile S. Le Duff ◽  
Marta Boter ◽  
Catarina Casais ◽  
Joanne E. Airey ◽  
...  
Keyword(s):  
Protein Interaction ◽  
Biochemical Characterization ◽  
Protein Protein Interaction ◽  
Dependent Protein

scholarly journals Phospho‐tyrosine dependent protein–protein interaction network

2015 ◽  
Vol 11 (3) ◽  
pp. 794 ◽  
Author(s):  
Arndt Grossmann ◽  
Nouhad Benlasfer ◽  
Petra Birth ◽  
Anna Hegele ◽  
Franziska Wachsmuth ◽  
...  
Keyword(s):  
Protein Interaction ◽  
Protein Interaction Network ◽  
Interaction Network ◽  
Protein Protein Interaction ◽  
Dependent Protein ◽  
Protein Protein Interaction Network

An efficient transient assays system using Agrobacterium-mediated transformation of onion (Allium cepa) epidermal cells

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Yu-Miao Zhang ◽  
Jun Wang ◽  
Tao Wu
Keyword(s):  
Subcellular Localization ◽  
Protein Interaction ◽  
Protein Interactions ◽  
Epidermal Cells ◽  
Cyclin Dependent Kinase ◽  
Protein Subcellular Localization ◽  
Protein Protein Interactions ◽  
Efficient System ◽  
Protein Protein Interaction ◽  
Onion Epidermal Cells

In this study, the Agrobacterium infection medium, infection duration, detergent, and cell density were optimized. The sorghum-based infection medium (SbIM), 10-20 min infection time, addition of 0.01% Silwet L-77, and Agrobacterium optical density at 600 nm (OD600), improved the competence of onion epidermal cells to support Agrobacterium infection at >90% efficiency. Cyclin-dependent kinase D-2 (CDKD-2) and cytochrome c-type biogenesis protein (CYCH), protein-protein interactions were localized. The optimized procedure is a quick and efficient system for examining protein subcellular localization and protein-protein interaction.

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