scholarly journals Enhanced Dopamine Release by Dopamine Transport Inhibitors Described by a Restricted Diffusion Model and Fast-Scan Cyclic Voltammetry

2016 ◽  
Vol 7 (6) ◽  
pp. 700-709 ◽  
Author(s):  
Alexander F. Hoffman ◽  
Charles E. Spivak ◽  
Carl R. Lupica
Keyword(s):  
Cyclic Voltammetry ◽  
Diffusion Model ◽  
Dopamine Release ◽  
Restricted Diffusion ◽  
Fast Scan Cyclic Voltammetry ◽  
Dopamine Transport ◽  
Transport Inhibitors

Striatal low-threshold spiking interneurons locally gate dopamine during learning

2020 ◽  
Author(s):  
Elizabeth N. Holly ◽  
M. Felicia Davatolhagh ◽  
Rodrigo A. España ◽  
Marc V. Fuccillo
Keyword(s):  
Cyclic Voltammetry ◽  
Dopamine Release ◽  
Striatal Dopamine ◽  
Operant Learning ◽  
Fast Scan Cyclic Voltammetry ◽  
Directed Learning ◽  
Low Threshold ◽  
Dorsomedial Striatum

Low-threshold spiking interneurons (LTSIs) in the dorsomedial striatum are potent modulators of goal-directed learning. Here, we uncover a novel function for LTSIs in locally and directly gating striatal dopamine, using in vitro fast scan cyclic voltammetry as well as in vivo GRAB-DA sensor imaging and pharmacology during operant learning. We demonstrate that LTSIs, acting via GABAB signaling, attenuate dopamine release, thereby serving as local coordinators of striatal plasticity.

scholarly journals Use of fast-scan cyclic voltammetry to assess phasic dopamine release in rat models of early postpartum maternal behavior and neglect

2017 ◽  
Vol 28 (8) ◽  
pp. 648-660 ◽  
Author(s):  
Tatiana A. Shnitko ◽  
Kyla D. Mace ◽  
Kaitlin M. Sullivan ◽  
W. Kyle Martin ◽  
Elizabeth H. Andersen ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Maternal Behavior ◽  
Dopamine Release ◽  
Fast Scan Cyclic Voltammetry ◽  
Rat Models ◽  
Early Postpartum

scholarly journals Development of the Wireless Instantaneous Neurotransmitter Concentration System for intraoperative neurochemical monitoring using fast-scan cyclic voltammetry

2009 ◽  
Vol 111 (4) ◽  
pp. 712-723 ◽  
Author(s):  
Jonathan M. Bledsoe ◽  
Christopher J. Kimble ◽  
Daniel P. Covey ◽  
Charles D. Blaha ◽  
Filippo Agnesi ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Carbon Fiber ◽  
Flow Injection ◽  
High Frequency ◽  
Dopamine Release ◽  
Functional Neurosurgery ◽  
Fast Scan Cyclic Voltammetry ◽  
Stimulation Of

Object Emerging evidence supports the hypothesis that modulation of specific central neuronal systems contributes to the clinical efficacy of deep brain stimulation (DBS) and motor cortex stimulation (MCS). Real-time monitoring of the neurochemical output of targeted regions may therefore advance functional neurosurgery by, among other goals, providing a strategy for investigation of mechanisms, identification of new candidate neurotransmitters, and chemically guided placement of the stimulating electrode. The authors report the development of a device called the Wireless Instantaneous Neurotransmitter Concentration System (WINCS) for intraoperative neurochemical monitoring during functional neurosurgery. This device supports fast-scan cyclic voltammetry (FSCV) at a carbon-fiber microelectrode (CFM) for real-time, spatially and chemically resolved neurotransmitter measurements in the brain. Methods The FSCV study consisted of a triangle wave scanned between −0.4 and 1 V at a rate of 300 V/second and applied at 10 Hz. All voltages were compared with an Ag/AgCl reference electrode. The CFM was constructed by aspirating a single carbon fiber (r = 2.5 μm) into a glass capillary and pulling the capillary to a microscopic tip by using a pipette puller. The exposed carbon fiber (that is, the sensing region) extended beyond the glass insulation by ~ 100 μm. The neurotransmitter dopamine was selected as the analyte for most trials. Proof-of-principle tests included in vitro flow injection and noise analysis, and in vivo measurements in urethane-anesthetized rats by monitoring dopamine release in the striatum following high-frequency electrical stimulation of the medial forebrain bundle. Direct comparisons were made to a conventional hardwired system. Results The WINCS, designed in compliance with FDA-recognized consensus standards for medical electrical device safety, consisted of 4 modules: 1) front-end analog circuit for FSCV (that is, current-to-voltage transducer); 2) Bluetooth transceiver; 3) microprocessor; and 4) direct-current battery. A Windows-XP laptop computer running custom software and equipped with a Universal Serial Bus–connected Bluetooth transceiver served as the base station. Computer software directed wireless data acquisition at 100 kilosamples/second and remote control of FSCV operation and adjustable waveform parameters. The WINCS provided reliable, high-fidelity measurements of dopamine and other neurochemicals such as serotonin, norepinephrine, and ascorbic acid by using FSCV at CFM and by flow injection analysis. In rats, the WINCS detected subsecond striatal dopamine release at the implanted sensor during high-frequency stimulation of ascending dopaminergic fibers. Overall, in vitro and in vivo testing demonstrated comparable signals to a conventional hardwired electrochemical system for FSCV. Importantly, the WINCS reduced susceptibility to electromagnetic noise typically found in an operating room setting. Conclusions Taken together, these results demonstrate that the WINCS is well suited for intraoperative neurochemical monitoring. It is anticipated that neurotransmitter measurements at an implanted chemical sensor will prove useful for advancing functional neurosurgery.

scholarly journals Mode of sucrose delivery alters reward-related phasic dopamine signals in nucleus accumbens

2017 ◽  
Author(s):  
James E McCutcheon ◽  
Mitchell F Roitman
Keyword(s):  
Cyclic Voltammetry ◽  
Nucleus Accumbens ◽  
Pavlovian Conditioning ◽  
Dopamine Release ◽  
Fast Scan Cyclic Voltammetry ◽  
Sensory Stimuli ◽  
Reward Seeking ◽  
Directed Action ◽  
External Stimuli

AbstractIn studies of appetitive Pavlovian conditioning, rewards are often delivered to subjects in a manner that confounds several processes. For example, delivery of a sugar pellet to a rodent requires movement to collect the pellet and is associated with sensory stimuli such as the sight and sound of the pellet arrival. Thus, any neurochemical events occurring in proximity to the reward may be related to multiple coincident phenomena. We used fast-scan cyclic voltammetry in rats to compare nucleus accumbens dopamine responses to two different modes of delivery: sucrose pellets, which require goal-directed action for their collection and are associated with sensory stimuli, and intraoral infusions of sucrose, which are passively received and not associated with external stimuli. We found that when rewards were unpredicted both pellets and infusions evoked similar dopamine release. However, when rewards were predicted by distinct cues, greater dopamine release was evoked by pellet cues than infusion cues. Thus, dopamine responses to pellets, infusions as well as predictive cues suggest a nuanced role for dopamine in both reward seeking and reward evaluation.

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