Molecular Force Sensors

2022 ◽  
Author(s):  
Rachel L. Bender ◽  
Khalid Salaita
Keyword(s):  
Force Sensors ◽  
Molecular Force

scholarly journals Molecular Force Sensors: From Fundamental Concepts toward Applications in Cell Biology

2016 ◽  
Vol 4 (1) ◽  
pp. 1600441 ◽  
Author(s):  
Melis Goktas ◽  
Kerstin G. Blank
Keyword(s):  
Cell Biology ◽  
Force Sensors ◽  
Molecular Force

scholarly journals Quantifying Molecular Forces with Serially Connected Force Sensors

2018 ◽  
Author(s):  
Y. Murad ◽  
I. T.S. Li
Keyword(s):  
Cell Adhesion ◽  
Dynamic Range ◽  
Computational Study ◽  
Experimental Studies ◽  
High Signal ◽  
Force Sensors ◽  
Adhesion Forces ◽  
Receptor Ligand ◽  
Sequence Composition ◽  
Molecular Force

AbstractTo understand the mechanical forces involved in cell adhesion, molecular force sensors have been developed to study tension through adhesion proteins. Recently, a class of molecular force sensors called tension gauge tether (TGT) have been developed that rely on irreversible force-dependent dissociation of DNA duplex to study cell adhesion forces. While the TGT offer high signal-to-noise ratio and is ideal for studying fast / single molecular adhesion processes, quantitative interpretation of experimental results has been challenging. Here we used computational approach to investigate how TGT fluorescence readout can be quantitatively interpreted. In particular we studied force sensors made of a single TGT, multiplexed single TGTs, and two TGTs connected in series. Our results showed that fluorescence readout using a single TGT can result from drastically different combinations of force history and adhesion event density that span orders of magnitude. In addition, the apparent behaviour of the TGT is influenced by the tethered receptor-ligand, making it necessary to calibrate the TGT with every new receptor-ligand. To solve this problem, we proposed a system of two serially connected TGTs. Our result shows that not only is the ratiometric readout of serial TGT independent of the choice of receptor-ligand, it is able to reconstruct force history with sub-pN force resolution. This is also not possible by simply multiplexing different types of TGTs together. Lastly, we systematically investigated how sequence composition of the two serially connected TGTs can be tuned to achieve different dynamic range. This computational study demonstrated how serially connected irreversible molecular dissociation processes can accurately quantify molecular force, and laid the foundation for subsequent experimental studies.

scholarly journals Extending the Capabilities of Molecular Force Sensors via DNA Nanotechnology

2020 ◽  
Vol 48 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Susana M. Beltrán ◽  
Marvin J. Slepian ◽  
Rebecca E. Taylor
Keyword(s):  
Dna Nanotechnology ◽  
Force Sensors ◽  
Molecular Force

Molecular force sensors to measure stress in cells

2017 ◽  
Vol 50 (23) ◽  
pp. 233001 ◽  
Author(s):  
Meenakshi Prabhune ◽  
Florian Rehfeldt ◽  
Christoph F Schmidt
Keyword(s):  
Force Sensors ◽  
Molecular Force ◽  
In Cells

scholarly journals Probing mechanotransduction in living cells by optical tweezers and FRET-based molecular force microscopy

2021 ◽  
Author(s):  
M. Sergides ◽  
L. Perego ◽  
T. Galgani ◽  
C. Arbore ◽  
F.S. Pavone ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Single Molecule ◽  
Single Cells ◽  
Force Sensors ◽  
Force Microscopy ◽  
Mechanical Signals ◽  
Cell Plasma ◽  
Molecular Force ◽  
Wide Range ◽  
Biochemical Signals

AbstractCells sense mechanical signals and forces to probe the external environment and adapt to tissue morphogenesis, external mechanical stresses, and a wide range of diverse mechanical cues. Here, we propose a combination of optical tools to manipulate single cells and measure the propagation of mechanical and biochemical signals inside them. Optical tweezers are used to trap microbeads that are used as handles to manipulate the cell plasma membrane; genetically encoded FRET-based force sensors inserted in F-actin and alpha-actinin are used to measure the propagation of mechanical signals to the cell cytoskeleton; while fluorescence microscopy with single molecule sensitivity can be used with a huge array of biochemical and genetic sensors. We describe the details of the setup implementation, the calibration of the basic components and preliminary characterization of actin and alpha-actinin FRET-based force sensors.

scholarly journals Probing mechanotransduction in living cells by optical tweezers and FRET-based molecular force microscopy

2021 ◽  
Vol 136 (3) ◽  
Author(s):  
M. Sergides ◽  
L. Perego ◽  
T. Galgani ◽  
C. Arbore ◽  
F. S. Pavone ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Single Molecule ◽  
Single Cells ◽  
Force Sensors ◽  
Force Microscopy ◽  
Mechanical Signals ◽  
Cell Plasma ◽  
Molecular Force ◽  
Wide Range ◽  
Biochemical Signals

AbstractCells sense mechanical signals and forces to probe the external environment and adapt to tissue morphogenesis, external mechanical stresses and a wide range of diverse mechanical cues. Here, we propose a combination of optical tools to manipulate single cells and measure the propagation of mechanical and biochemical signals inside them. Optical tweezers are used to trap microbeads that are used as handles to manipulate the cell plasma membrane; genetically encoded FRET-based force sensors inserted in F-actin and alpha-actinin are used to measure the propagation of mechanical signals to the cell cytoskeleton, while fluorescence microscopy with single-molecule sensitivity can be used with a huge array of biochemical and genetic sensors. We describe the details of the setup implementation, the calibration of the basic components and preliminary characterization of actin and alpha-actinin FRET-based force sensors.

Molecular force field evaluation by empirical constraints

1976 ◽  
Vol 73 ◽  
pp. 1051-1057
Author(s):  
Sadao Isotani ◽  
Alain J.-P. Alix
Keyword(s):  
Force Field ◽  
Field Evaluation ◽  
Molecular Force ◽  
Molecular Force Field

Calibration of dynamic force sensors by the deformation method

2019 ◽  
pp. 15-19
Author(s):  
F. V. Bulygin ◽  
M. Y. Prilepko
Keyword(s):  
Force Sensors ◽  
Dynamic Force ◽  
Deformation Method
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