Stimulated Emission Depletion (STED) Super-Resolution Imaging with an Advanced Organic Fluorescent Probe: Visualizing the Cellular Lipid Droplets at the Unprecedented Nanoscale Resolution

2021 ◽  
pp. 516-524
Author(s):  
Ri Zhou ◽  
Chenguang Wang ◽  
Xishuang Liang ◽  
Fangmeng Liu ◽  
Xu Yan ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Lipid Droplets ◽  
Super Resolution ◽  
Stimulated Emission ◽  
Cellular Lipid ◽  
Resolution Imaging ◽  
Stimulated Emission Depletion

Recent advances in luminescent materials for super-resolution imaging via stimulated emission depletion nanoscopy

2021 ◽  
Author(s):  
Yanzi Xu ◽  
Ruohan Xu ◽  
Zhi Wang ◽  
Yu Zhou ◽  
Qifei Shen ◽  
...  
Keyword(s):  
Recent Progress ◽  
Super Resolution ◽  
Stimulated Emission ◽  
Luminescent Materials ◽  
New Materials ◽  
Recent Advances ◽  
Resolution Imaging ◽  
Stimulated Emission Depletion

Recent progress on STED fluorophores for super-resolution imaging and also their characteristics are outlined here, thus providing some guidelines to select proper probes and even develop new materials for super-resolution imaging via STED nanoscopy.

Long-term STED imaging of amyloid fibers with exchangeable Thioflavin T

Nanoscale ◽  
2020 ◽  
Vol 12 (28) ◽  
pp. 15050-15053
Author(s):  
Joaquim Torra ◽  
Patricia Bondia ◽  
Sylvia Gutierrez-Erlandsson ◽  
Begoña Sot ◽  
Cristina Flors
Keyword(s):  
Super Resolution ◽  
Stimulated Emission ◽  
Thioflavin T ◽  
Amyloid Fibers ◽  
Resolution Imaging ◽  
Stimulated Emission Depletion

We report the use of the amyloid probe Thioflavin T (ThT) as a specific and exchangeable fluorophore for stimulated emission depletion (STED) super-resolution imaging of amyloid fibers.

scholarly journals Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Maria J. Sarmento ◽  
Michele Oneto ◽  
Simone Pelicci ◽  
Luca Pesce ◽  
Lorenzo Scipioni ◽  
...  
Keyword(s):  
Super Resolution ◽  
Stimulated Emission ◽  
Resolution Imaging ◽  
Stimulated Emission Depletion ◽  
Nuclear Structures

scholarly journals Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell Imaging

Biosensors ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 330
Author(s):  
Jia Zhang ◽  
Xinwei Gao ◽  
Luwei Wang ◽  
Yong Guo ◽  
Yinru Zhu ◽  
...  
Keyword(s):  
Low Power ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Super Resolution ◽  
Live Cell ◽  
Stimulated Emission ◽  
Live Cells ◽  
Sted Microscopy ◽  
Resolution Imaging ◽  
Stimulated Emission Depletion

Stimulated emission depletion (STED) microscopy is a typical laser-scanning super-resolution imaging technology, the emergence of which has opened a new research window for studying the dynamic processes of live biological samples on a nanometer scale. According to the characteristics of STED, a high depletion power is required to obtain a high resolution. However, a high laser power can induce severe phototoxicity and photobleaching, which limits the applications for live cell imaging, especially in two-color STED super-resolution imaging. Therefore, we developed a low-power two-color STED super-resolution microscope with a single supercontinuum white-light laser. Using this system, we achieved low-power two-color super-resolution imaging based on digital enhancement technology. Lateral resolutions of 109 and 78 nm were obtained for mitochondria and microtubules in live cells, respectively, with 0.8 mW depletion power. These results highlight the great potential of the novel digitally enhanced two-color STED microscopy for long-term dynamic imaging of live cells.

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