Light Emission Properties of Thermally Evaporated CH3NH3PbBr3 Perovskite from Nano- to Macro-Scale: Role of Free and Localized Excitons

Over the past decade, interest about metal halide perovskites has rapidly increased, as they can find wide application in optoelectronic devices. Nevertheless, although thermal evaporation is crucial for the development and engineering of such devices based on multilayer structures, the optical properties of thermally deposited perovskite layers (spontaneous and amplified spontaneous emission) have been poorly investigated. This paper is a study from a nano- to micro- and macro-scale about the role of light-emitting species (namely free carriers and excitons) and trap states in the spontaneous emission of thermally evaporated thin layers of CH3NH3PbBr3 perovskite after wet air UV light trap passivation. The map of light emission from grains, carried out by SNOM at the nanoscale and by micro-PL techniques, clearly indicates that free and localized excitons (EXs) are the dominant light-emitting species, the localized excitons being the dominant ones in the presence of crystallites. These species also have a key role in the amplified spontaneous emission (ASE) process: for higher excitation densities, the relative contribution of localized EXs basically remains constant, while a clear competition between ASE and free EXs spontaneous emission is present, which suggests that ASE is due to stimulated emission from the free EXs.

SPITFIR(e): A supermaneuverable algorithm for restoring 2D-3D fluorescence images and videos, and background subtraction

While fluorescent microscopy imaging has become the spearhead of modern biology as it is able to generate long-term videos depicting 4D nanoscale cell behaviors, it is still limited by the optical aberrations and the photon budget available in the specimen and to some extend to photo-toxicity. A direct consequence is the necessity to develop flexible and "off-road" algorithms in order to recover structural details and improve spatial resolution, which is critical when pushing the illumination to the low levels in order to limit photo-damages. Moreover, as the processing of very large temporal series of images considerably slows down the analysis, special attention must be paid to the feasibility and scalability of the developed restoration algorithms. To address these specifications, we present a very flexible method designed to restore 2D-3D+Time fluorescent images and subtract undesirable out-of-focus background. We assume that the images are sparse and piece-wise smooth, and are corrupted by mixed Poisson-Gaussian noise. To recover the unknown image, we consider a novel convex and non-quadratic regularizer Sparse Hessian Variation) defined as the mixed norms which gathers image intensity and spatial second-order derivatives. This resulting restoration algorithm named SPITFIR(e) (SParse fIT for Fluorescence Image Restoration) utilizes the primal-dual optimization principle for energy minimization and can be used to process large images acquired with varied fluorescence microscopy modalities. It is nearly parameter-free as the practitioner needs only to specify the amount of desired sparsity (weak, moderate, high). Experimental results in lattice light sheet, stimulated emission depletion, multifocus microscopy, spinning disk confocal, and wide-field microscopy demonstrate the generic ability of the SPITFIR(e) algorithm to efficiently reduce noise and blur, and to subtract undesirable fluorescent background, while avoiding the emergence of deconvolution artifacts.

Spectral and Transmittance Properties of Er3+ Doped Zinc Lithium Lead Calcium Borophosphate Glasses

Zinc lithium lead calcium borophosphate glasses containing Er3+ in (40- x):P2O5:10ZnO:10Li2O:10PbO:10CaO:20B2O3:xEr2O3 (where x=1, 1.5,2 mol %) have been prepared by melt-quenching method. The amorphous nature of the glasses was confirmed by x-ray diffraction studies. Optical absorption, Excitation, fluorescence and Transmittance spectra were recorded at room temperature for all glass samples. Judd-Ofelt intensity parameters Ωλ (λ=2, 4, 6) are evaluated from the intensities of various absorption bands of optical absorption spectra. Using these intensity parameters various radiative properties like spontaneous emission probability, branching ratio, radiative life time and stimulated emission cross–section of various emission lines have been evaluated. Keywords: ZLLCBP Glasses, Optical Properties, Judd-OfeltTheory, Transmittance Properties.

Development of TRINETRA : A Sensor Based Vision Enhancement System for Obstacle Detection on Railway Tracks

The authors have developed a prototype, which promises easy passage for trains in unknown environments such as zero visibility situations, fog, smog, and heavy rain. The prototype is based upon the integration of a camera, Radio Detection and Ranging (RADAR), and Infrared (IR) Light Amplification by Stimulated Emission of Radiation (LASER).The camera used in the prototype catches a long-range view of the track and presents it live on a mini screen fixed in the loco pilot cabin. A combined short, mid, and long-range radar sensor system is used to detect obstacles continuously in loco pilot blind spots on the track, particularly for collision avoidance assistance at high speed. The present work proposes a long-range laser IR illuminator with a wide range of color and mono cameras to aid clear and precise monitoring in zero visibility conditions, which is fixed on the front portion/engine of the locomotive/engine. The prototype experimental results for 2 m - 2 km distances have been performed on a live running train, which shows that the developed prototype tracks obstacles effectively during fog and smog conditions. The design concept, observation, prototype model, and other technical specifications have been presented, and satisfactory results were found.

Development of TRINETRA : A Sensor Based Vision Enhancement System for Obstacle Detection on Railway Tracks

The authors have developed a prototype, which promises easy passage for trains in unknown environments such as zero visibility situations, fog, smog, and heavy rain. The prototype is based upon the integration of a camera, Radio Detection and Ranging (RADAR), and Infrared (IR) Light Amplification by Stimulated Emission of Radiation (LASER).The camera used in the prototype catches a long-range view of the track and presents it live on a mini screen fixed in the loco pilot cabin. A combined short, mid, and long-range radar sensor system is used to detect obstacles continuously in loco pilot blind spots on the track, particularly for collision avoidance assistance at high speed. The present work proposes a long-range laser IR illuminator with a wide range of color and mono cameras to aid clear and precise monitoring in zero visibility conditions, which is fixed on the front portion/engine of the locomotive/engine. The prototype experimental results for 2 m - 2 km distances have been performed on a live running train, which shows that the developed prototype tracks obstacles effectively during fog and smog conditions. The design concept, observation, prototype model, and other technical specifications have been presented, and satisfactory results were found.

Polarized spectroscopy of electric and magnetic dipole transitions of Europium (III) ions in C2 sites

Polarization anisotropy of luminescent properties of europium (III) ions in low-symmetry C2 sites is studied using monoclinic (sp. gr. C2/c) tungstate crystal KY(WO4)2. The 5D0 → 7FJ (where J = 0…6) transitions are characterized for the principal light polarizations. Polarization selection rules for the magnetic dipole 5D0 → 7F1 transition are presented. The stimulated-emission cross-sections for Eu3+ ions relevant for laser operation are determined.

Radiation of argon clustered flow particles outside the exciting electron beam

The spectral study results of a supersonic clustered argon flow stimulated emission in the region of particle excitation (on an electron beam) and downstream are presented. Anomalies in the luminescence of the traditional spindle-shaped jet and the flow of heavy clusters (“wake”) are discussed. Possible causes of the observed anomalous phenomena are presented on the basis of the given comparisons of the results obtained.

Nonspecific binding of common anti-CFTR antibodies in ciliated cells of human airway epithelium

There is evidence that the cystic fibrosis transmembrane conductance regulator (CFTR) anion channel is highly expressed at the apical pole of ciliated cells in human bronchial epithelium (HBE), however recent studies have detected little CFTR mRNA in those cells. To understand this discrepancy we immunostained well differentiated primary HBE cells using CFTR antibodies. We confirmed apical immunofluorescence in ciliated cells and quantified the covariance of the fluorescence signals and that of an antibody against the ciliary marker centrin-2 using image cross-correlation spectroscopy (ICCS). Super-resolution stimulated emission depletion (STED) imaging localized the immunofluorescence in distinct clusters at the bases of the cilia. However, similar apical fluorescence was observed when the monoclonal CFTR antibodies 596, 528 and 769 were used to immunostain ciliated cells expressing F508del-CFTR, or cells lacking CFTR due to a Class I mutation. A BLAST search using the CFTR epitope identified a similar amino acid sequence in the ciliary protein rootletin X1. Its expression level correlated with the intensity of immunostaining by CFTR antibodies and it was detected by 596 antibody after transfection into CFBE cells. These results may explain the high apparent expression of CFTR in ciliated cells and reports of anomalous apical immunofluorescence in well differentiated cells that express F508del-CFTR.