Intraparticle Energy Level Alignment of Semiconducting Polymer Nanoparticles to Amplify Chemiluminescence for Ultrasensitive In Vivo Imaging of Reactive Oxygen Species

ACS Nano ◽  
2016 ◽  
Vol 10 (6) ◽  
pp. 6400-6409 ◽  
Author(s):  
Xu Zhen ◽  
Chengwu Zhang ◽  
Chen Xie ◽  
Qingqing Miao ◽  
Kah Leong Lim ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Energy Level ◽  
In Vivo Imaging ◽  
Reactive Oxygen ◽  
Polymer Nanoparticles ◽  
Oxygen Species ◽  
Semiconducting Polymer ◽  
Energy Level Alignment ◽  
Semiconducting Polymer Nanoparticles

In Vivo Imaging of Ca2+, pH, and Reactive Oxygen Species Using Fluorescent Probes in Plants

2011 ◽  
Vol 62 (1) ◽  
pp. 273-297 ◽  
Author(s):  
Sarah J. Swanson ◽  
Won-Gyu Choi ◽  
Alexandra Chanoca ◽  
Simon Gilroy
Keyword(s):  
Reactive Oxygen Species ◽  
Fluorescent Probes ◽  
In Vivo Imaging ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Anti-Inflammatory and Reactive Oxygen Species Suppression through Aspirin Pretreatment to Treat Hyperoxia-Induced Acute Lung Injury in NF-κB–Luciferase Inducible Transgenic Mice

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 429 ◽  
Author(s):  
Chuan-Mu Chen ◽  
Yu-Tang Tung ◽  
Chi-Hsuan Wei ◽  
Po-Ying Lee ◽  
Wei Chen
Keyword(s):  
Reactive Oxygen Species ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Transgenic Mice ◽  
In Vivo Imaging ◽  
Reactive Oxygen ◽  
Luciferase Expression ◽  
Oxygen Species ◽  
Anti Inflammatory

Acute lung injury (ALI), a common cause of morbidity and mortality in intensive care units, results from either direct intra-alveolar injury or indirect injury following systemic inflammation and oxidative stress. Adequate tissue oxygenation often requires additional supplemental oxygen. However, hyperoxia causes lung injury and pathological changes. Notably, preclinical data suggest that aspirin modulates numerous platelet-mediated processes involved in ALI development and resolution. Our previous study suggested that prehospital aspirin use reduced the risk of ALI in critically ill patients. This research uses an in vivo imaging system (IVIS) to investigate the mechanisms of aspirin’s anti-inflammatory and antioxidant effects on hyperoxia-induced ALI in nuclear factor κB (NF-κB)–luciferase transgenic mice. To define mechanisms through which NF-κB causes disease, we developed transgenic mice that express luciferase under the control of NF-κB, enabling real-time in vivo imaging of NF-κB activity in intact animals. An NF-κB-dependent bioluminescent signal was used in transgenic mice carrying the luciferase genes to monitor the anti-inflammatory effects of aspirin. These results demonstrated that pretreatment with aspirin reduced luciferase expression, indicating that aspirin reduces NF-κB activation. In addition, aspirin reduced reactive oxygen species expression, the number of macrophages, neutrophil infiltration and lung edema compared with treatment with only hyperoxia treatment. In addition, we demonstrated that pretreatment with aspirin significantly reduced the protein levels of phosphorylated protein kinase B, NF-κB and tumor necrosis factor α in NF-κB–luciferase+/+ transgenic mice. Thus, the effects of aspirin on the anti-inflammatory response and reactive oxygen species suppressive are hypothesized to occur through the NF-κB signaling pathway. This study demonstrated that aspirin exerts a protective effect for hyperoxia-induced lung injury and thus is currently the drug conventionally used for hyperoxia-induced lung injury.

scholarly journals Reactive Oxygen Species as a Response to Wounding: In Vivo Imaging in Arabidopsis thaliana

2020 ◽  
Vol 10 ◽  
Author(s):  
Ankush Prasad ◽  
Michaela Sedlářová ◽  
Anastasiia Balukova ◽  
Marek Rác ◽  
Pavel Pospíšil
Keyword(s):  
Reactive Oxygen Species ◽  
Arabidopsis Thaliana ◽  
In Vivo Imaging ◽  
Reactive Oxygen ◽  
Oxygen Species

Catalytic Chemiluminescence Polymer Dots for Ultrasensitive In Vivo Imaging of Intrinsic Reactive Oxygen Species in Mice

2018 ◽  
Vol 90 (11) ◽  
pp. 6929-6935 ◽  
Author(s):  
Lvping Cai ◽  
Liyun Deng ◽  
Xiangyi Huang ◽  
Jicun Ren
Keyword(s):  
Reactive Oxygen Species ◽  
In Vivo Imaging ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Polymer Dots

Radiosynthesis of an 18 F-fluoroglycosylated aminoferrocene for in-vivo imaging of reactive oxygen species activity by PET

2018 ◽  
Vol 61 (14) ◽  
pp. 1081-1088 ◽  
Author(s):  
Johannes Toms ◽  
Viktor Reshetnikov ◽  
Simone Maschauer ◽  
Andriy Mokhir ◽  
Olaf Prante
Keyword(s):  
Reactive Oxygen Species ◽  
In Vivo Imaging ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Species Activity

scholarly journals In Vivo Imaging of Retinal Oxidative Stress Using a Reactive Oxygen Species–Activated Fluorescent Probe

2015 ◽  
Vol 56 (10) ◽  
pp. 5862 ◽  
Author(s):  
Megan C. Prunty ◽  
Moe H. Aung ◽  
Adam M. Hanif ◽  
Rachael S. Allen ◽  
Micah A. Chrenek ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Fluorescent Probe ◽  
In Vivo Imaging ◽  
Reactive Oxygen ◽  
Oxygen Species

Fluorescent in vivo imaging of reactive oxygen species and redox potential in plants

2018 ◽  
Vol 122 ◽  
pp. 202-220 ◽  
Author(s):  
Cristina Ortega-Villasante ◽  
Stefan Burén ◽  
Alfonso Blázquez-Castro ◽  
Ángel Barón-Sola ◽  
Luis E. Hernández
Keyword(s):  
Reactive Oxygen Species ◽  
Redox Potential ◽  
In Vivo Imaging ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Targeting autophagy enhances atezolizumab-induced mitochondria-related apoptosis in osteosarcoma

2021 ◽  
Vol 12 (2) ◽  
Author(s):  
Zhuochao Liu ◽  
Hongyi Wang ◽  
Chuanzhen Hu ◽  
Chuanlong Wu ◽  
Jun Wang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Antitumor Immunity ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Specific Monoclonal Antibody ◽  
Osteosarcoma Cells ◽  
Jnk Pathway ◽  
In Vivo Experiments

AbstractIn this study, we identified the multifaceted effects of atezolizumab, a specific monoclonal antibody against PD-L1, in tumor suppression except for restoring antitumor immunity, and investigated the promising ways to improve its efficacy. Atezolizumab could inhibit the proliferation and induce immune-independent apoptosis of osteosarcoma cells. With further exploration, we found that atezolizumab could impair mitochondria of osteosarcoma cells, resulting in increased release of reactive oxygen species and cytochrome-c, eventually leading to mitochondrial-related apoptosis via activating JNK pathway. Nevertheless, the excessive release of reactive oxygen species also activated the protective autophagy of osteosarcoma cells. Therefore, when we combined atezolizumab with autophagy inhibitors, the cytotoxic effect of atezolizumab on osteosarcoma cells was significantly enhanced in vitro. Further in vivo experiments also confirmed that atezolizumab combined with chloroquine achieved the most significant antitumor effect. Taken together, our study indicates that atezolizumab can induce mitochondrial-related apoptosis and protective autophagy independently of the immune system, and targeting autophagy is a promising combinatorial approach to amplify its cytotoxicity.

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