High-Level Production of the Natural Blue Pigment Indigoidine from Metabolically Engineered Corynebacterium glutamicum for Sustainable Fabric Dyes

2021 ◽  
Author(s):  
Mohammad Rifqi Ghiffary ◽  
Cindy Pricilia Surya Prabowo ◽  
Komal Sharma ◽  
Yuchun Yan ◽  
Sang Yup Lee ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Blue Pigment ◽  
High Level ◽  
Level Production

Fermentative High-Level Production of 5-Hydroxyvaleric Acid by Metabolically Engineered Corynebacterium glutamicum

2021 ◽  
Vol 9 (6) ◽  
pp. 2523-2533
Author(s):  
Yu Jung Sohn ◽  
Minsoo Kang ◽  
Kei-Anne Baritugo ◽  
Jina Son ◽  
Kyoung Hee Kang ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Hydroxyvaleric Acid ◽  
High Level ◽  
Level Production

High-level production of Bacillus cereus phospholipase C in Corynebacterium glutamicum

2015 ◽  
Vol 216 ◽  
pp. 142-148 ◽  
Author(s):  
Pablo Ravasi ◽  
Mauricio Braia ◽  
Florencia Eberhardt ◽  
Claudia Elena ◽  
Sebastián Cerminati ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Phospholipase C ◽  
Corynebacterium Glutamicum ◽  
High Level ◽  
Level Production

Metabolic Engineering of Corynebacterium glutamicum for the High-Level Production of Cadaverine That Can Be Used for the Synthesis of Biopolyamide 510

2018 ◽  
Vol 6 (4) ◽  
pp. 5296-5305 ◽  
Author(s):  
Hee Taek Kim ◽  
Kei-Anne Baritugo ◽  
Young Hoon Oh ◽  
Sung Min Hyun ◽  
Tae Uk Khang ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Corynebacterium Glutamicum ◽  
High Level ◽  
Level Production

Protein body-inducing fusions for high-level production and purification of recombinant proteins in plants

2011 ◽  
Vol 9 (4) ◽  
pp. 419-433 ◽  
Author(s):  
Andrew J. Conley ◽  
Jussi J. Joensuu ◽  
Alex Richman ◽  
Rima Menassa
Keyword(s):  
Recombinant Proteins ◽  
Protein Body ◽  
High Level ◽  
Level Production

scholarly journals High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

2018 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Roselei Claudete Fontana ◽  
Simone Mendonça ◽  
Félix Gonçalves de Siqueira ◽  
Aldo José Pinheiro Dillon ◽  
...  
Keyword(s):  
Nitrogen Sources ◽  
White Rot ◽  
Stirred Tank ◽  
Carbon And Nitrogen Sources ◽  
Stirred Tank Bioreactor ◽  
Carbon And Nitrogen ◽  
High Level ◽  
Level Production

Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris

2001 ◽  
Vol 86 (1) ◽  
pp. 59-70 ◽  
Author(s):  
Stefan Minning ◽  
Alicia Serrano ◽  
Pau Ferrer ◽  
Carles Solá ◽  
Rolf D. Schmid ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Rhizopus Oryzae ◽  
Rhizopus Oryzae Lipase ◽  
High Level ◽  
Level Production

scholarly journals RamB, a Novel Transcriptional Regulator of Genes Involved in Acetate Metabolism of Corynebacterium glutamicum

2004 ◽  
Vol 186 (9) ◽  
pp. 2798-2809 ◽  
Author(s):  
Robert Gerstmeir ◽  
Annette Cramer ◽  
Petra Dangel ◽  
Steffen Schaffer ◽  
Bernhard J. Eikmanns
Keyword(s):  
Corynebacterium Glutamicum ◽  
Isocitrate Lyase ◽  
Transcriptional Regulator ◽  
Regulatory Elements ◽  
Malate Synthase ◽  
Acetate Kinase ◽  
Acetate Metabolism ◽  
Peptide Mass ◽  
Specific Activities ◽  
High Level

ABSTRACT The adaptation of Corynebacterium glutamicum to acetate as a carbon and energy source involves transcriptional regulation of the pta-ack operon coding for the acetate-activating enzymes phosphotransacetylase and acetate kinase and of the aceA and aceB genes coding for the glyoxylate cycle enzymes isocitrate lyase and malate synthase, respectively. Deletion and mutation analysis of the respective promoter regions led to the identification of highly conserved 13-bp motifs (AA/GAACTTTGCAAA) as cis-regulatory elements for expression of the pta-ack operon and the aceA and aceB genes. By use of DNA affinity chromatography, a 53-kDa protein specifically binding to the promoter/operator region of the pta-ack operon was purified. Mass spectrometry and peptide mass fingerprinting identified the protein as a putative transcriptional regulator (which was designated RamB). Purified His-tagged RamB protein was shown to bind specifically to both the pta-ack and the aceA/aceB promoter/operator regions. Directed deletion of the ramB gene in the genome of C. glutamicum resulted in mutant strain RG1. Whereas the wild type of C. glutamicum showed high-level specific activities of acetate kinase, phosphotransacetylase, isocitrate lyase, and malate synthase when grown on acetate and low-level specific activities when grown on glucose as sole carbon and energy sources, mutant RG1 showed high-level specific activities with all four enzymes irrespective of the substrate. Comparative transcriptional cat fusion experiments revealed that this deregulation takes place at the level of transcription. The results indicate that RamB is a negative transcriptional regulator of genes involved in acetate metabolism of C. glutamicum.

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