Location of the Catalytic Site for Phosphoenolpyruvate Formation within the Primary Structure of Clostridium symbiosum Pyruvate Phosphate Dikinase. 1. Identification of an Essential Cysteine by Chemical Modification with [1-14C]Bromopyruvate and Site-Directed Mutagenesis

Biochemistry ◽  
1995 ◽  
Vol 34 (7) ◽  
pp. 2181-2187 ◽  
Author(s):  
Yuan Xu ◽  
Linda Yankie ◽  
Li Shen ◽  
Young-Shik Jung ◽  
Patrick S. Mariano ◽  
...  
Keyword(s):  
Chemical Modification ◽  
Primary Structure ◽  
Catalytic Site ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Pyruvate Phosphate Dikinase ◽  
Clostridium Symbiosum

Structure-Function Studies of Escherichia coli Biotin Synthase via a Chemical Modification and Site-Directed Mutagenesis Approach

2001 ◽  
Vol 130 (5) ◽  
pp. 627-635 ◽  
Author(s):  
L. Farh ◽  
S.-Y. Hwang ◽  
L. Steinrauf ◽  
H.-J. Chiang ◽  
D. Shiuan
Keyword(s):  
Escherichia Coli ◽  
Chemical Modification ◽  
Structure Function ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Biotin Synthase

Controlled Site-Selective Glycosylation of Proteins by a Combined Site-Directed Mutagenesis and Chemical Modification Approach

1998 ◽  
Vol 63 (26) ◽  
pp. 9614-9615 ◽  
Author(s):  
Benjamin G. Davis ◽  
Richard C. Lloyd ◽  
J. Bryan Jones
Keyword(s):  
Chemical Modification ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Site Selective

Primary Structure, Expression, and Site-Directed Mutagenesis of Inorganic Pyrophosphatase from Bacillus stearothermophilus

1999 ◽  
Vol 125 (1) ◽  
pp. 48-57 ◽  
Author(s):  
T. Satoh ◽  
H. Shinoda ◽  
K. Ishii ◽  
M. Koyama ◽  
N. Sakurai ◽  
...  
Keyword(s):  
Primary Structure ◽  
Bacillus Stearothermophilus ◽  
Site Directed Mutagenesis ◽  
Inorganic Pyrophosphatase ◽  
Directed Mutagenesis
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