Role of gpFI Protein in DNA Packaging by Bacteriophage .lambda.

Carlo Enrique Catalano; Mary Ann Tomka

doi:10.1021/bi00031a027

THE INTERACTION OF cos WITH CHI IS SEPARABLE FROM DNA PACKAGING IN recA-recBC-MEDIATED RECOMBINATION OF BACTERIOPHAGE LAMBDA

Genetics ◽

10.1093/genetics/104.4.549 ◽

1983 ◽

Vol 104 (4) ◽

pp. 549-570

Author(s):

Ichizo Kobayashi ◽

Mary M Stahl ◽

David Leach ◽

Franklin W Stahl

Keyword(s):

Escherichia Coli ◽

Bacteriophage Lambda ◽

Dna Packaging ◽

Bacteriophage Λ ◽

Entry Site ◽

Two Component ◽

General Recombination

ABSTRACT Chi (5′-GCTGGTGG) is a recombinator in RecA- RecBC-mediated recombination in Escherichia coli. In bacteriophage λ vegetative recombination, Chi is fully active only when it is correctly oriented with respect to cos, the site that defines the ends of the packaged chromosome. Here we demonstrate that packaging from cos is not necessary for this cos-Chi interaction. Our evidence suggests that correctly oriented cos is an activator of Chi. cos, as an activator, is (1) dominant over cos -, (2) active opposite an extensive heterology, (3) able to interact with Chi only when on the same (cis) chromosome, and (4) able to interact with Chi at distances as far as ≥ 20 kb. Thus, cos and Chi form a two-component recombinator system for general recombination. cos may serve as an asymmetric entry site for a recombination enzyme that recognizes Chi in an asymmetric way.

Role of the major capsid protein of phage T4 in DNA packaging from structure-function and site-directed mutagenesis studies

Journal of Structural Biology ◽

10.1016/1047-8477(90)90060-p ◽

1990 ◽

Vol 104 (1-3) ◽

pp. 75-83 ◽

Cited By ~ 2

Author(s):

Min Quan Xue ◽

Lindsay W. Black

Keyword(s):

Structure Function ◽

Capsid Protein ◽

Major Capsid Protein ◽

Dna Packaging ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Phage T4

Studies of DNA packaging into the heads of bacteriophage lambda

Journal of Molecular Biology ◽

10.1016/0022-2836(78)90044-x ◽

1978 ◽

Vol 126 (3) ◽

pp. 333-346 ◽

Cited By ~ 9

Author(s):

Michael Syvanen ◽

Jerry Yin

Keyword(s):

Bacteriophage Lambda ◽

Dna Packaging

The role of gene Nu3 in bacteriophage lambda head morphogenesis

Virology ◽

10.1016/0042-6822(75)90096-3 ◽

1975 ◽

Vol 64 (1) ◽

pp. 247-263 ◽

Cited By ~ 57

Author(s):

Peter Ray ◽

Helios Murialdo

Keyword(s):

Bacteriophage Lambda

Visualization of the intracellular development of bacteriophage lambda, with special reference to DNA packaging.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.75.7.3206 ◽

1978 ◽

Vol 75 (7) ◽

pp. 3206-3210 ◽

Cited By ~ 3

Author(s):

H. Yamagishi ◽

M. Okamoto

Keyword(s):

Special Reference ◽

Bacteriophage Lambda ◽

Dna Packaging

Helical-repeat dependence of integrative recombination of bacteriophage lambda: role of the P1 and H1 protein binding sites.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.85.17.6323 ◽

1988 ◽

Vol 85 (17) ◽

pp. 6323-6327 ◽

Cited By ~ 11

Author(s):

J. F. Thompson ◽

U. K. Snyder ◽

A. Landy

Keyword(s):

Protein Binding ◽

Binding Sites ◽

Bacteriophage Lambda ◽

Protein Binding Sites

Role of S gene of bacteriophage lambda in host lysis

Biochemical and Biophysical Research Communications ◽

10.1016/0006-291x(76)91142-6 ◽

1976 ◽

Vol 70 (1) ◽

pp. 302-309 ◽

Cited By ~ 5

Author(s):

P.K. Mukherjee ◽

R.K. Mandal

Keyword(s):

Bacteriophage Lambda ◽

S Gene ◽

Host Lysis

The Role of cosB, the Binding Site for Terminase, the DNA Packaging Enzyme of Bacteriophage λ in the Nicking Reaction

Journal of Molecular Biology ◽

10.1006/jmbi.1993.1614 ◽

1993 ◽

Vol 234 (3) ◽

pp. 594-609 ◽

Cited By ~ 20

Author(s):

David Cue ◽

Michael Feiss

Keyword(s):

Binding Site ◽

Dna Packaging ◽

Bacteriophage Λ ◽

Dna Packaging Enzyme

Role of Genetic Recombination in DNA Replication of Bacteriophage Lambda II. Effect in DNA Replication by Gene Delta

Journal of Virology ◽

10.1128/jvi.14.6.1451-1457.1974 ◽

1974 ◽

Vol 14 (6) ◽

pp. 1451-1457 ◽

Cited By ~ 3

Author(s):

K. Barta ◽

J. Zissler

Keyword(s):

Dna Replication ◽

Genetic Recombination ◽

Bacteriophage Lambda

COUPLING WITH PACKAGING EXPLAINS APPARENT NONRECIPROCALITY OF CHI-STIMULATED RECOMBINATION OF BACTERIOPHAGE LAMBDA BY RECA AND RECBC FUNCTIONS

Genetics ◽

10.1093/genetics/108.4.773 ◽

1984 ◽

Vol 108 (4) ◽

pp. 773-794

Author(s):

Ichizo Kobayashi ◽

Mary M Stahl ◽

Frederic R Fairfield ◽

Franklin W Stahl

Keyword(s):

Escherichia Coli ◽

Bacteriophage Lambda ◽

Coupling Model ◽

Strand Break ◽

Double Strand Break ◽

Dna Packaging ◽

The Other ◽

Entry Site ◽

Strong Candidate

ABSTRACT Chi (Χ, 5'-GCTGGTGG) is a recombinator in RecA- and RecBC-mediated recombination in Escherichia coli. In vegetative recombination between two bacteriophage lambda strains, one with and the other without Chi (a +Χ+ b - × a-Χob+), the Χ-containing recombinant (a -Χ+ b -) is less abundant than the non-Χ-containing recombinant (a+Χob+). Previously this was taken was evidence for nonreciprocality of Χ-stimulated exchange. This inequality, however, is now seen to result from an event at cos (λ's packaging origin) that both activates Chi and initiates DNA packaging. An event at rightward cos leads to activation of leftward Χ on the same chromosome for an exchange to its left. From the resulting circulating dimer (-cos-a+-Χo-b+-cos-a--Χ+-b—), the cos that activated Χ is more likely to be used for rightward packaging initiation than is the cos from the other parent. Consistent with this coupling model is "biased packaging" in λ carrying two cos sites per monomer genome. When their maturation is dependent on dimerization by Χ-stimulated exchange, the phage particles result more often from packaging from the cos that activates Χ than from packaging from the other cos. Since Chi activation and packaging can be uncoupled, we infer that some early and reversible step in packaging activates Χ. A strong candidate for this step is a double-strand break at cos that provides an oriented entry site for a recombinase.