Purification and immunogenicity of fusion VP1 protein of foot and mouth disease virus

Biochemistry ◽  
1984 ◽  
Vol 23 (26) ◽  
pp. 6474-6480 ◽  
Author(s):  
Steven J. Shire ◽  
Larry Bock ◽  
John Ogez ◽  
Stuart Builder ◽  
Dennis Kleid ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Vp1 Protein ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Expression and Immunogenicity of Two Recombinant Fusion Proteins Comprising Foot-and-Mouth Disease Virus Structural Protein VP1 and DC-SIGN-Binding Glycoproteins

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Xinsheng Liu ◽  
Jianliang Lv ◽  
Yuzhen Fang ◽  
Peng Zhou ◽  
Yanzhen Lu ◽  
...  
Keyword(s):  
Immune Responses ◽  
Disease Virus ◽  
Fusion Proteins ◽  
Insect Cells ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Vp1 Protein ◽  
Sf9 Insect Cells ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Improving vaccine immunogenicity by targeting antigens to dendritic cells has recently emerged as a new design strategy in vaccine development. In this study, the VP1 gene of foot-and-mouth disease virus (FMDV) serotype A was fused with the gene encoding human immunodeficiency virus (HIV) membrane glycoprotein gp120 or C2-V3 domain of hepatitis C virus (HCV) envelope glycoprotein E2, both of which are DC-SIGN-binding glycoproteins. After codon optimization, the VP1 protein and the two recombinant VP1-gp120 and VP1-E2 fusion proteins were expressed in Sf9 insect cells using the insect cell-baculovirus expression system. Western blotting showed that the VP1 protein and two recombinant VP1-gp120 and VP1-E2 fusion proteins were correctly expressed in the Sf9 insect cells and had good reactogenicity. Guinea pigs were then immunized with the purified proteins, and the resulting humoral and cellular immune responses were analyzed. The VP1-gp120 and VP1-E2 fusion proteins induced significantly higher specific anti-FMDV antibody levels than the VP1 protein and stronger cell-mediated immune responses. This study provides a new perspective for the development of novel FMDV subunit vaccines.

Preparation and characterization of monoclonal antibodies against VP1 protein of foot-and-mouth disease virus O/China99

2009 ◽  
Vol 24 (6) ◽  
pp. 566-572
Author(s):  
Shuai Song ◽  
Tong Lin ◽  
Jun-jun Shao ◽  
Shan-dian Gao ◽  
Guo-zheng Cong ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Vp1 Protein ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Application of VP1 protein to develop monoclonal antibody against foot-and-mouth disease virus Asia1 type

2009 ◽  
Vol 24 (3) ◽  
pp. 215-220 ◽  
Author(s):  
Tong Lin ◽  
Jun-zheng Du ◽  
Jun-jun Shao ◽  
Guo-zheng Cong ◽  
Shuai Song ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Vp1 Protein ◽  
Mouth Disease Virus ◽  
Foot And Mouth

VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display

2013 ◽  
Vol 175 (1) ◽  
pp. 87-90 ◽  
Author(s):  
A. Peralta ◽  
G.A. Maroniche ◽  
V. Alfonso ◽  
P. Molinari ◽  
O. Taboga
Keyword(s):  
Disease Virus ◽  
Surface Display ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Vp1 Protein ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Identification of a conformational neutralizing epitope on the VP1 protein of type A foot-and-mouth disease virus

2017 ◽  
Vol 115 ◽  
pp. 374-381 ◽  
Author(s):  
Wenming Liu ◽  
Baolin Yang ◽  
Mingxia Wang ◽  
Haiwei Wang ◽  
Decheng Yang ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Type A ◽  
Mouth Disease ◽  
Neutralizing Epitope ◽  
Vp1 Protein ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Production of foot-and-mouth disease virus SAT2 VP1 protein

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mpho Victoria Mamabolo ◽  
Jacques Theron ◽  
Francois Maree ◽  
Michael Crampton
Keyword(s):  
Disease Virus ◽  
Inclusion Bodies ◽  
Foot And Mouth Disease ◽  
Luria Broth ◽  
Mouth Disease ◽  
Fed Batch ◽  
Vp1 Protein ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Shake Flasks

AbstractThe seven serotypes of foot-and-mouth disease virus (FMDV) differ on the surface exposed regions on the VP1, 2 and 3 proteins. Amongst the three, the VP1 protein has been produced the most for use in serotyping assays for some of the Euro-Asian serotypes. In this study the VP1 protein of the FMDV SAT2/ZIM/7/83 was expressed in Escherichia coli BL21 cells in Luria broth and EnPresso® B media in shake flasks. Production was further developed and the VP1 protein was produced at 2.15 g L−1 in fed-batch fermentations at 2 L scale. The protein formed insoluble inclusion bodies that were isolated, denatured and refolded. When tested in ELISA, the protein was found to be highly reactive with serum from a SAT2 vaccinated guinea pig, and not reactive to SAT1 and SAT3 antisera. These results open avenues to evaluate recombinantly expressed VP1 proteins for differentiation of the three Southern African Territories serotypes of FMDV that co-occur in Southern and East Africa. In addition, this could mitigate the need for employing virus as reagent, or having to raise reagent antibodies.

Field outbreak strains of serotype O foot-and-mouth disease virus from India with a deletion in the immunodominant βG-βH loop of the VP1 protein

2012 ◽  
Vol 157 (10) ◽  
pp. 1967-1970 ◽  
Author(s):  
Biswajit Das ◽  
Aniket Sanyal ◽  
Saravanan Subramaniam ◽  
Jajati K. Mohapatra ◽  
Bramhadev Pattnaik
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Vp1 Protein ◽  
Serotype O ◽  
Mouth Disease Virus ◽  
Foot And Mouth
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