In situ enzymology of DNA replication and ultraviolet-induced DNA repair synthesis in permeable human cells

Biochemistry ◽  
1988 ◽  
Vol 27 (19) ◽  
pp. 7247-7254 ◽  
Author(s):  
Steven L. Dresler ◽  
Mark G. Frattini ◽  
Rona M. Robinson-Hill
Keyword(s):  
Dna Repair ◽  
Dna Replication ◽  
Human Cells ◽  
Repair Synthesis ◽  
Dna Repair Synthesis

Visualization of Focal Nuclear Sites of DNA Repair Synthesis Induced by Bleomycin in Human Cells

2001 ◽  
Vol 156 (4) ◽  
pp. 347-354 ◽  
Author(s):  
N. V. Tomilin ◽  
L. V. Solovjeva ◽  
M. P. Svetlova ◽  
N. M. Pleskach ◽  
I. A. Zalenskaya ◽  
...  
Keyword(s):  
Dna Repair ◽  
Human Cells ◽  
Repair Synthesis ◽  
Dna Repair Synthesis

scholarly journals Aphidicolin does not inhibit DNA repair synthesis in ultraviolet-irradiated HeLa cells. A radioautographic study

1981 ◽  
Vol 199 (2) ◽  
pp. 453-455 ◽  
Author(s):  
N Hardt ◽  
G Pedrali-Noy ◽  
F Focher ◽  
S Spadari
Keyword(s):  
Dna Repair ◽  
Dna Replication ◽  
Dna Synthesis ◽  
Dna Polymerase ◽  
Hela Cells ◽  
Nuclear Dna ◽  
Detectable Effect ◽  
Repair Synthesis ◽  
Dna Polymerase Alpha ◽  
Dna Repair Synthesis

A radioautographic examination of nuclear DNA synthesis in unirradiated and u.v.-irradiated HeLa cells, in the presence and in the absence of aphidicolin, showed that aphidicolin inhibits nuclear DNA replication and has no detectable effect on DNA repair synthesis. Although the results establish that in u.v.-irradiated HeLa cells most of the DNA repair synthesis is not due to DNA polymerase alpha, they do not preclude a significant role for this enzyme in DNA repair processes.

scholarly journals TRAIP drives replisome disassembly and mitotic DNA repair synthesis at sites of incomplete DNA replication

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Remi Sonneville ◽  
Rahul Bhowmick ◽  
Saskia Hoffmann ◽  
Niels Mailand ◽  
Ian D Hickson ◽  
...  
Keyword(s):  
Dna Repair ◽  
Dna Replication ◽  
Ubiquitin Ligase ◽  
Early Embryo ◽  
Model System ◽  
Replication Forks ◽  
Nematode Caenorhabditis Elegans ◽  
Repair Synthesis ◽  
Dna Repair Synthesis ◽  
Early Mitosis

The faithful segregation of eukaryotic chromosomes in mitosis requires that the genome be duplicated completely prior to anaphase. However, cells with large genomes sometimes fail to complete replication during interphase and instead enter mitosis with regions of incompletely replicated DNA. These regions are processed in early mitosis via a process known as mitotic DNA repair synthesis (MiDAS), but little is known about how cells switch from conventional DNA replication to MiDAS. Using the early embryo of the nematode Caenorhabditis elegans as a model system, we show that the TRAIP ubiquitin ligase drives replisome disassembly in response to incomplete DNA replication, thereby providing access to replication forks for other factors. Moreover, TRAIP is essential for MiDAS in human cells, and is important in both systems to prevent mitotic segregation errors. Our data indicate that TRAIP is a master regulator of the processing of incomplete DNA replication during mitosis in metazoa.

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