Biochemical Characterization of MmoS, a Sensor Protein Involved in Copper-Dependent Regulation of Soluble Methane Monooxygenase†

Biochemistry ◽  
2006 ◽  
Vol 45 (34) ◽  
pp. 10191-10198 ◽  
Author(s):  
Uchechi E. Ukaegbu ◽  
Shannon Henery ◽  
Amy C. Rosenzweig
Keyword(s):  
Biochemical Characterization ◽  
Methane Monooxygenase ◽  
Soluble Methane Monooxygenase ◽  
Sensor Protein

Purification and Biochemical Characterization of Soluble Methane Monooxygenase Hydroxylase fromMethylosinus trichosporiumIMV 3011

2007 ◽  
Vol 71 (1) ◽  
pp. 122-129 ◽  
Author(s):  
Hua SHAOFENG ◽  
Li SHUBEN ◽  
Xin JIAYIN ◽  
Niu JIANZHONG ◽  
Xia CHUNGU ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Methane Monooxygenase ◽  
Soluble Methane Monooxygenase

scholarly journals Purification and Characterization of the Soluble Methane Monooxygenase of the Type II Methanotrophic BacteriumMethylocystis sp. Strain WI 14

1999 ◽  
Vol 65 (9) ◽  
pp. 3929-3935 ◽  
Author(s):  
Stephan Grosse ◽  
Louise Laramee ◽  
Karin-Dagmar Wendlandt ◽  
Ian R. McDonald ◽  
Carlos B. Miguez ◽  
...  
Keyword(s):  
Methane Monooxygenase ◽  
Enzyme Regulation ◽  
Absorption Spectrometry ◽  
Type Ii ◽  
Methylococcus Capsulatus ◽  
Oxidation Of Methane ◽  
Soluble Methane Monooxygenase ◽  
Molecular Masses ◽  
High Degree

ABSTRACT Methane monooxygenase (MMO) catalyzes the oxidation of methane to methanol as the first step of methane degradation. A soluble NAD(P)H-dependent methane monooxygenase (sMMO) from the type II methanotrophic bacterium WI 14 was purified to homogeneity. Sequencing of the 16S rDNA and comparison with that of other known methanotrophic bacteria confirmed that strain WI 14 is very close to the genusMethylocystis. The sMMO is expressed only during growth under copper limitation (<0.1 μM) and with ammonium or nitrate ions as the nitrogen source. The enzyme exhibits a low substrate specificity and is able to oxidize several alkanes and alkenes, cyclic hydrocarbons, aromatics, and halogenic aromatics. It has three components, hydroxylase, reductase and protein B, which is involved in enzyme regulation and increases sMMO activity about 10-fold. The relative molecular masses of the native components were estimated to be 229, 41, and 18 kDa, respectively. The hydroxylase contains three subunits with relative molecular masses of 57, 43, and 23 kDa, which are present in stoichiometric amounts, suggesting that the native protein has an α2β2γ2structure. We detected 3.6 mol of iron per mol of hydroxylase by atomic absorption spectrometry. sMMO is strongly inhibited by Hg2+ions (with a total loss of enzyme activity at 0.01 mM Hg2+) and Cu2+, Zn2+, and Ni2+ ions (95, 80, and 40% loss of activity at 1 mM ions). The complete sMMO gene sequence has been determined. sMMO genes from strain WI 14 are clustered on the chromosome and show a high degree of homology (at both the nucleotide and amino acid levels) to the corresponding genes fromMethylosinus trichosporium OB3b, Methylocystissp. strain M, and Methylococcus capsulatus (Bath).

Plant Pathology Diagnosed by X-Ray Microanalysis

1987 ◽  
Vol 45 ◽  
pp. 974-975
Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang
Keyword(s):  
Electron Microscopy ◽  
Plant Pathology ◽  
Biochemical Characterization ◽  
Nutrient Deficiency ◽  
Green Leaf ◽  
Parasitic Infestation ◽  
X Ray

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.

Interaction effect of rootstocks on gas exchange parameters, biochemical changes and nutrientstatus in Sauvignon Blanc winegrapes

2014 ◽  
Vol 3 (3) ◽  
pp. 218-225
Author(s):  
R. G. Somkuwar ◽  
M. A. Bhange ◽  
A. K. Upadhyay ◽  
S. D. Ramteke
Keyword(s):  
Biochemical Characterization ◽  
Reducing Sugar ◽  
Wine Grape ◽  
Gas Exchange Parameters ◽  
Food Material ◽  
Total Carbohydrates ◽  
Salt Creek ◽  
Grape Varieties ◽  
Photosynthetic Activities

SauvignonBlanc wine grape was characterized for their various morphological, physiological and biochemical parameters grafted on different rootstocks. Significant differences were recorded for all the parameters studied. The studies on vegetative parameters revealed that the rootstock influences the vegetative growth thereby increasing the photosynthetic activities of a vine. The highest photosynthesis rate was recorded in 140-Ru grafted vine followed by Fercal whereas the lowest in Salt Creek rootstock grafted vines.The rootstock influenced the changes in biochemical constituents in the grafted vine thereby helping the plant to store enough food material. Significant differences were recorded for total carbohydrates, proteins, total phenols and reducing sugar. The vines grafted on1103-Pshowed highest carbohydrates and starch followed by 140-Ru,while the least amount of carbohydrates were recorded in 110-R and Salt Creek grafted vines respectively.Among the different rootstock graft combinations, Fercal showed highest amount of reducing sugar, proteins and phenols, followed by 1103-P and SO4, however, the lowest amount of reducing sugar, proteins and phenols were recorded with 110-R grafted vines.The vines grafted on different rootstocks showed changes in nutrient uptake. Considering this, the physico-biochemical characterization of grafted vine may help to identify particularrootstocks combination that could influence a desired trait in commercial wine grape varieties after grafting.

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