Biochemical Characterization of Recombinant Hepatitis C Virus Nonstructural Protein 4B: Evidence for ATP/GTP Hydrolysis and Adenylate Kinase Activity†

Biochemistry ◽  
2009 ◽  
Vol 48 (5) ◽  
pp. 906-916 ◽  
Author(s):  
Aaron A. Thompson ◽  
Aihua Zou ◽  
Jiangli Yan ◽  
Rohit Duggal ◽  
Weidong Hao ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Adenylate Kinase ◽  
Kinase Activity ◽  
Biochemical Characterization ◽  
Nonstructural Protein ◽  
Recombinant Hepatitis ◽  
Gtp Hydrolysis

scholarly journals Morphological and Biochemical Characterization of the Membranous Hepatitis C Virus Replication Compartment

2013 ◽  
Vol 87 (19) ◽  
pp. 10612-10627 ◽  
Author(s):  
D. Paul ◽  
S. Hoppe ◽  
G. Saher ◽  
J. Krijnse-Locker ◽  
R. Bartenschlager
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Replication ◽  
Biochemical Characterization ◽  
Replication Compartment

scholarly journals Identification and Biochemical Characterization of Halisulfate 3 and Suvanine as Novel Inhibitors of Hepatitis C Virus NS3 Helicase from a Marine Sponge

Marine Drugs ◽  
2014 ◽  
Vol 12 (1) ◽  
pp. 462-476 ◽  
Author(s):  
Atsushi Furuta ◽  
Kazi Salam ◽  
Idam Hermawan ◽  
Nobuyoshi Akimitsu ◽  
Junichi Tanaka ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Marine Sponge ◽  
Biochemical Characterization

Identification and characterization of a natural inter-genotypic (2b/1b) recombinant hepatitis C virus in Japan

2011 ◽  
Vol 156 (9) ◽  
pp. 1591-1601 ◽  
Author(s):  
Koji Yokoyama ◽  
Masaharu Takahashi ◽  
Tsutomu Nishizawa ◽  
Shigeo Nagashima ◽  
Suljid Jirintai ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Recombinant Hepatitis ◽  
Identification And Characterization

Characterization of the nuclear localization signal and subcellular distribution of hepatitis C virus nonstructural protein NS5A

Gene ◽  
1996 ◽  
Vol 182 (1-2) ◽  
pp. 203-211 ◽  
Author(s):  
Yoshihiro Ide ◽  
Luwen Zhang ◽  
Min Chen ◽  
Genevieve Inchauspe ◽  
Chander Bahl ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Nuclear Localization ◽  
Nuclear Localization Signal ◽  
Subcellular Distribution ◽  
Nonstructural Protein ◽  
Localization Signal

Characterization of recombinant hepatitis C virus NS5B gene product (RDRP)

1997 ◽  
Vol 34 (2) ◽  
pp. A93
Author(s):  
CH Hagedorn ◽  
RH Al ◽  
C De Staercke ◽  
Y Wang ◽  
Y Xie
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Gene Product ◽  
Recombinant Hepatitis

scholarly journals Characterization of Nonstructural Protein Membrane Anchor Deletion Mutants Expressed in the Context of the Hepatitis C Virus Polyprotein

2005 ◽  
Vol 79 (12) ◽  
pp. 7911-7917 ◽  
Author(s):  
Rainer Gosert ◽  
Wiebke Jendrsczok ◽  
Jan Martin Berke ◽  
Volker Brass ◽  
Hubert E. Blum ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Protein Interactions ◽  
Nonstructural Protein ◽  
Protein Protein Interactions ◽  
Nonstructural Proteins ◽  
Membrane Anchor ◽  
Replication Complex ◽  
Cytosolic Domains

ABSTRACT Protein-protein interactions involved in formation of the membrane-associated hepatitis C virus (HCV) replication complex are poorly understood. Here, we investigated nonstructural proteins with deletions in their membrane anchor domains when expressed in the context of the entire HCV polyprotein. Interactions among cytosolic domains of HCV nonstructural proteins were found not to be sufficiently strong to rescue such mutants to the membrane. Thus, the membrane anchor domains of nonstructural proteins are essential for incorporation of these proteins into the HCV replication complex while interactions among the cytosolic domains appear to be relatively weak. This feature may provide the nonstructural proteins with a certain flexibility to perform their multiple functions during HCV replication.

scholarly journals Newly Synthesized Hepatitis C Virus Replicon RNA Is Protected from Nuclease Activity by a Protease-Sensitive Factor(s)

2004 ◽  
Vol 78 (18) ◽  
pp. 10202-10205 ◽  
Author(s):  
Guang Yang ◽  
Daniel C. Pevear ◽  
Marc S. Collett ◽  
Srinivas Chunduru ◽  
Dorothy C. Young ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Lipid Membranes ◽  
Biochemical Characterization ◽  
Buoyant Density ◽  
Nuclease Activity ◽  
Replication Complex ◽  
Sensitive Factor ◽  
Protease Treatment

ABSTRACT Biochemical characterization of hepatitis C virus (HCV) replication using purified, membrane-associated replication complexes is hampered by the presence of endogenous nuclease activity that copurifies with the replication complex. In this study, pulse-chase analyses were used to demonstrate that newly synthesized replicon RNA was protected from nuclease activity by a factor(s) that was sensitive to 0.5% NP-40 or protease treatment. Nuclease susceptibility was not related to disruption of lipid membranes, since NP-40 did not significantly affect the buoyant density of HCV replication complexes or protease susceptibility of HCV NS3 and NS5A proteins. These results suggest that a protease-sensitive factor(s) protects newly synthesized RNA from nuclease degradation.

Isolation and characterization of RNA aptamers specific for the hepatitis C virus nonstructural protein 3 protease

2000 ◽  
Vol 267 (12) ◽  
pp. 3685-3694 ◽  
Author(s):  
Kotaro Fukuda ◽  
Daesety Vishnuvardhan ◽  
Satoru Sekiya ◽  
Joonsung Hwang ◽  
Nobuko Kakiuchi ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Nonstructural Protein ◽  
Rna Aptamers ◽  
Isolation And Characterization ◽  
Nonstructural Protein 3
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