Mutagenesis of Acidic Residues in the Oxygenase Domain of Inducible Nitric-Oxide Synthase Identifies a Glutamate Involved in Arginine Binding†

Biochemistry ◽  
1997 ◽  
Vol 36 (17) ◽  
pp. 5097-5103 ◽  
Author(s):  
Ratan Gachhui ◽  
Dipak K. Ghosh ◽  
Chaoqun Wu ◽  
John Parkinson ◽  
Brian R. Crane ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Acidic Residues ◽  
Oxygenase Domain ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Dissociation and unfolding of inducible nitric oxide synthase oxygenase domain identifies structural role of tetrahydrobiopterin in modulating the heme environment

2006 ◽  
Vol 284 (1-2) ◽  
pp. 117-126 ◽  
Author(s):  
Rajib Sengupta ◽  
Rupam Sahoo ◽  
Sougata Sinha Ray ◽  
Tanmay Dutta ◽  
Anjan Dasgupta ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Structural Role ◽  
Oxygenase Domain ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Characterization of the Inducible Nitric Oxide Synthase Oxygenase Domain Identifies a 49 Amino Acid Segment Required for Subunit Dimerization and Tetrahydrobiopterin Interaction†

Biochemistry ◽  
1997 ◽  
Vol 36 (35) ◽  
pp. 10609-10619 ◽  
Author(s):  
Dipak K. Ghosh ◽  
Chaoqun Wu ◽  
Eva Pitters ◽  
Michael Moloney ◽  
Ernst R. Werner ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Amino Acid ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Oxygenase Domain ◽  
Oxide Synthase ◽  
Amino Acid Segment ◽  
Inducible Nitric Oxide

Stoichiometric Arginine Binding in the Oxygenase Domain of Inducible Nitric Oxide Synthase Requires a Single Molecule of Tetrahydrobiopterin per Dimer

1999 ◽  
Vol 257 (2) ◽  
pp. 344-347 ◽  
Author(s):  
Steven P. Rafferty ◽  
Jeffrey C. Boyington ◽  
Rachel Kulansky ◽  
Peter D. Sun ◽  
Harry L. Malech
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Single Molecule ◽  
Oxygenase Domain ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Kinetics of CO binding to the haem domain of murine inducible nitric oxide synthase: differential effects of haem domain ligands

2001 ◽  
Vol 358 (1) ◽  
pp. 201-208 ◽  
Author(s):  
Thirza H. STEVENSON ◽  
Aldo F. GUTIERREZ ◽  
Wendy K. ALDERTON ◽  
Lu-yun LIAN ◽  
Nigel S. SCRUTTON
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Active Site ◽  
Type I ◽  
Geminate Recombination ◽  
Oxygenase Domain ◽  
Oxide Synthase ◽  
Haem Iron ◽  
Inducible Nitric Oxide

The binding of CO to the murine inducible nitric oxide synthase (iNOS) oxygenase domain has been studied by laser flash photolysis. The effect of the (6R)-5,6,7,8-tetrahydro-l-biopterin (BH4) cofactor l-arginine and several Type I l-arginine analogues/ligands on the rates of CO rebinding has been evaluated. The presence of BH4 in the iNOS active site has little effect on the rebinding of protein-caged haem–CO pairs (geminate recombination), but decreases the bimolecular association rates 2-fold. Addition of l-arginine to the BH4-bound complex completely abolishes geminate recombination and results in a further 80-fold decrease in the overall rate of bimolecular association. Three of the Type I ligands, S-ethylisothiourea, l-canavanine and 2,5-lutidine, displaced the CO from the haem iron upon addition to the iNOS oxygenase domain. The Type I ligands significantly decreased the rate of bimolecular binding of CO to the haem iron after photolysis. Most of these ligands also completely abolished geminate recombination. These results are consistent with a relatively open distal pocket that allows CO to bind unhindered in the active site of murine iNOS in the absence of l-arginine or BH4. In the presence of BH4 and l-arginine, however, the enzyme adopts a more closed structure that can greatly reduce ligand access to the haem iron. These observations are discussed in terms of the known structure of iNOS haem domain and solution studies of ligand binding in iNOS and neuronal NOS isoenzymes.

scholarly journals Mutations in the FMN Domain Modulate MCD Spectra of the Heme Site in the Oxygenase Domain of Inducible Nitric Oxide Synthase

2009 ◽  
Vol 131 (20) ◽  
pp. 6940-6941 ◽  
Author(s):  
Joseph Sempombe ◽  
Bradley O. Elmore ◽  
Xi Sun ◽  
Andrea Dupont ◽  
Dipak K. Ghosh ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Oxygenase Domain ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Helicobacter pylori enhances an expression of inducible nitric oxide synthase (iNOS) in human gastric epithelium

2001 ◽  
Vol 120 (5) ◽  
pp. A653-A654
Author(s):  
Q SONG ◽  
M OWENS ◽  
D SMOOT ◽  
H ASHKTORAB ◽  
B GOLD
Keyword(s):  
Nitric Oxide ◽  
Helicobacter Pylori ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Gastric Epithelium ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide
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