Selective Deamidation of Recombinant Human Stem Cell Factor during in Vitro Aging:  Isolation and Characterization of the Aspartyl and Isoaspartyl Homodimers and Heterodimers

Abstract Recombinant human stem cell factor (SCF) is homologous with recombinant rat SCF (rrSCF) and is a ligand for c-kit. We determined the influence of SCF on hematopoiesis in vitro and in vivo in baboons. In vitro, SCF alone stimulated little growth of hematopoietic colony-forming cells from baboon marrow, but did increase the number of colonies formed in response to erythropoietin (Epo), interleukin-3 (IL-3), and granulocyte- macrophage colony-stimulating factor (GM-CSF). In vivo, SCF caused an increase in the peripheral blood of the number of erythrocytes, neutrophils, lymphocytes, monocytes, eosinophils, and basophils. In marrow, it caused an increase in marrow cellularity and in the absolute number of colony-forming unit-granulocyte-monocyte (CFU-GM) and burst- forming unit-erythroid (BFU-E) in marrow following infusion of SCF. The in vivo stimulation of multiple lymphohematopoietic lineages corroborates previous in vitro studies and suggests a potentially important clinical role for SCF.

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Human stem cell factor does not affect the morphology and expression of functionally relevant molecules of Langerhans cells in vitro

Journal of Dermatological Science ◽

10.1016/s0923-1811(98)83569-x ◽

1998 ◽

Vol 16 ◽

pp. S96

Author(s):

Francesca Prignano ◽

Gianni Gerlini ◽

Nicola Pimpinelli ◽

Paolo Romagnoli ◽

Benvenuto Giannotti

Keyword(s):

Stem Cell ◽

Stem Cell Factor ◽

Langerhans Cells ◽

Human Stem Cell ◽

Human Stem Cell Factor

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164. Effect of recombinant human stem cell factor on the proliferation and differentiation in vitro of hemopoietic progenitors in myeiodisplastic syndromes

Biomedicine & Pharmacotherapy ◽

10.1016/0753-3322(92)90249-7 ◽

1992 ◽

Vol 46 (5-7) ◽

pp. 322

Author(s):

W Piacibello ◽

F Sanavio ◽

L Fubini ◽

S Morelli ◽

A Séverine ◽

...

Keyword(s):

Stem Cell ◽

Stem Cell Factor ◽

Human Stem Cell ◽

Proliferation And Differentiation ◽

Human Stem Cell Factor

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Recombinant human stem cell factor, a c-kit ligand, stimulates hematopoiesis in primates

Blood ◽

10.1182/blood.v78.8.1975.bloodjournal7881975 ◽

1991 ◽

Vol 78 (8) ◽

pp. 1975-1980 ◽

Cited By ~ 1

Author(s):

RG Andrews ◽

GH Knitter ◽

SH Bartelmez ◽

KE Langley ◽

D Farrar ◽

...

Keyword(s):

Stem Cell ◽

Stem Cell Factor ◽

Absolute Number ◽

Human Stem Cell ◽

Clinical Role ◽

Gm Csf ◽

Macrophage Colony ◽

Human Stem Cell Factor

Recombinant human stem cell factor (SCF) is homologous with recombinant rat SCF (rrSCF) and is a ligand for c-kit. We determined the influence of SCF on hematopoiesis in vitro and in vivo in baboons. In vitro, SCF alone stimulated little growth of hematopoietic colony-forming cells from baboon marrow, but did increase the number of colonies formed in response to erythropoietin (Epo), interleukin-3 (IL-3), and granulocyte- macrophage colony-stimulating factor (GM-CSF). In vivo, SCF caused an increase in the peripheral blood of the number of erythrocytes, neutrophils, lymphocytes, monocytes, eosinophils, and basophils. In marrow, it caused an increase in marrow cellularity and in the absolute number of colony-forming unit-granulocyte-monocyte (CFU-GM) and burst- forming unit-erythroid (BFU-E) in marrow following infusion of SCF. The in vivo stimulation of multiple lymphohematopoietic lineages corroborates previous in vitro studies and suggests a potentially important clinical role for SCF.

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Expression, refolding, and characterization of a novel recombinant dual human stem cell factor

Protein Expression and Purification ◽

10.1016/j.pep.2005.03.014 ◽

2005 ◽

Vol 43 (2) ◽

pp. 126-132 ◽

Cited By ~ 7

Author(s):

Haiqin Lu ◽

Yuhui Zang ◽

Yuguan Ze ◽

Jie Zhu ◽

Tao Chen ◽

...

Keyword(s):

Stem Cell ◽

Stem Cell Factor ◽

Human Stem Cell ◽

Human Stem Cell Factor

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Xenogeneic expression of human stem cell factor in transgenic mice mimics codominant c-kit mutations

Blood ◽

10.1182/blood.v87.8.3203.bloodjournal8783203 ◽

1996 ◽

Vol 87 (8) ◽

pp. 3203-3211 ◽

Cited By ~ 9

Author(s):

MK Majumdar ◽

ET Everett ◽

X Xiao ◽

R Cooper ◽

K Langley ◽

...

Keyword(s):

Stem Cell ◽

Transgenic Mice ◽

Stem Cell Factor ◽

Coat Color ◽

Protein Product ◽

Cell Surface Expression ◽

Human Stem Cell ◽

Human Stem Cell Factor

Mutations of c-kit, which encodes a transmembrane receptor tyrosine kinase, have been identified in mice by abnormal coat color, anemia, and germ cell defects. Mice heterozygous for mutations of c-kit have a white forehead blaze and a white ventral spot, leading these mutants to be termed dominant White spotting (W). We have previously demonstrated that the membrane-associated isoform of human stem cell factor (hSCF220, the ligand for c-kit) is inefficiently processed in murine stromal cell transfectants. Thus, in murine cell lines analyzed in vitro, hSCF220 transfectants present SCF as a membrane restricted protein in contrast to the murine SCF220 cDNA protein product, which is slowly cleaved and secreted. We show here that transgenic mice expressing the human SCF220 isoform in vivo display a phenotype indistinguishable from some alleles of W. Specifically, hSCF220- expressing transgenic mice display a prominent forehead blaze and a white ventral spot. Generations of doubly heterozygous animals that carry both a mutated c-kit allele and the hSCF220 transgene display a more severe coat color abnormality. This phenotype appears to be due to occupancy of murine c-kit by human SCF and diminished cell surface expression of endogenous murine SCF. Normal signaling events that lead to cell survival or proliferation appear to be disrupted in vivo in these transgenic mice.

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