Electrostatic Channeling of Oxaloacetate in a Fusion Protein of Porcine Citrate Synthase and Porcine Mitochondrial Malate Dehydrogenase†

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The substrate (l-malate) gradient created in a microfluidic channel induced the directional diffusion of mitochondrial malate dehydrogenase (mMDH) toward a higher concentration region and in situ generation of an intermediate (OAA) gradient enhanced the co-diffusion of citrate synthase (CS) together with mMDH.



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Pietro P. M. Iannetta ◽  
Nieves Medina Escobar ◽  
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