Self-Assembly of Elastin-Based Peptides into the ECM: the Importance of Integrins and the Elastin Binding Protein in Elastic Fiber Assembly

2011 ◽  
Vol 12 (2) ◽  
pp. 432-440 ◽  
Author(s):  
Dhaval Patel ◽  
Rohan Menon ◽  
Lakeshia J. Taite
Keyword(s):  
Self Assembly ◽  
Binding Protein ◽  
Elastic Fiber ◽  
Fiber Assembly ◽  
Elastin Binding Protein

scholarly journals Normal Wound Healing in Mice Deficient for Fibulin-5, an Elastin Binding Protein Essential for Dermal Elastic Fiber Assembly

2006 ◽  
Vol 126 (12) ◽  
pp. 2707-2714 ◽  
Author(s):  
Qian Zheng ◽  
Jiwon Choi ◽  
Leonie Rouleau ◽  
Richard L. Leask ◽  
James A. Richardson ◽  
...  
Keyword(s):  
Wound Healing ◽  
Binding Protein ◽  
Elastic Fiber ◽  
Fiber Assembly ◽  
Normal Wound Healing ◽  
Elastin Binding Protein

scholarly journals 67-kD elastin-binding protein is a protective "companion" of extracellular insoluble elastin and intracellular tropoelastin.

1994 ◽  
Vol 126 (2) ◽  
pp. 563-574 ◽  
Author(s):  
A Hinek ◽  
M Rabinovitch
Keyword(s):  
Binding Protein ◽  
Light And Electron Microscopy ◽  
Serine Proteinase ◽  
Elastic Fibers ◽  
Fiber Assembly ◽  
Elastin Fiber ◽  
Alternatively Spliced ◽  
Elastin Binding Protein ◽  
Highly Hydrophobic

The 67-kD elastin-binding protein (EBP) mediates cell adhesion to elastin and elastin fiber assembly, and it is similar, if not identical, to the 67-kD enzymatically inactive, alternatively spliced beta-galactosidase. The latter contains an elastin binding domain (S-GAL) homologous both to the aorta EBP and to NH2-terminal sequences of serine proteinases (Hinek, A., M. Rabinovitch, F. W. Keeley, and J. Callahan. 1993. J. Clin. Invest. 91:1198-1205). We now confirm the functional importance of this homology by showing that elastolytic activity of a representative serine elastase, porcine pancreatic elastase, was prevented by an antibody (anti-S-GAL) and by competing with purified EBP or S-GAL peptide. Immunohistochemistry of adult aorta indicates that the EBP exists as a permanent component of mature elastic fibers. This observation, together with the in vitro studies, suggests that the EBP could protect insoluble elastin from extracellular proteolysis and contribute to the extraordinary stability of this protein. Double immunolabeling of fetal lamb aorta with anti-S-GAL and antitropoelastin antibodies demonstrated, under light and electron microscopy, intracellular colocalization of the proteins in smooth muscle cells (SMC). Incubation of SMC with galactosugars to dissociate tropoelastin from EBP caused intracellular aggregation of tropoelastin. A tropoelastin/EBP complex was extracted from SMC lysates by coimmunoprecipitation and cross-linking, and its functional significance was addressed by showing that its dissociation by galactosugars caused degradation of tropoelastin by endogenous serine proteinase(s). This suggests that the EBP may also serve as a "companion" to intracellular tropoelastin, protecting this highly hydrophobic protein from self-aggregation and proteolytic degradation.

scholarly journals Latent TGF-  binding protein 4 promotes elastic fiber assembly by interacting with fibulin-5

2013 ◽  
Vol 110 (8) ◽  
pp. 2852-2857 ◽  
Author(s):  
K. Noda ◽  
B. Dabovic ◽  
K. Takagi ◽  
T. Inoue ◽  
M. Horiguchi ◽  
...  
Keyword(s):  
Binding Protein ◽  
Elastic Fiber ◽  
Fiber Assembly

scholarly journals Latent TGF-β-binding protein 2 binds to DANCE/fibulin-5 and regulates elastic fiber assembly

2007 ◽  
Vol 26 (14) ◽  
pp. 3283-3295 ◽  
Author(s):  
Maretoshi Hirai ◽  
Masahito Horiguchi ◽  
Tetsuya Ohbayashi ◽  
Toru Kita ◽  
Kenneth R Chien ◽  
...  
Keyword(s):  
Binding Protein ◽  
Elastic Fiber ◽  
Fiber Assembly

scholarly journals Enhancement of elastin expression by transdermal administration of sialidase isozyme Neu2

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Akira Minami ◽  
Yuka Fujita ◽  
Jun Goto ◽  
Ayano Iuchi ◽  
Kosei Fujita ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Skin Diseases ◽  
Lower Layer ◽  
Elastic Fiber ◽  
Sialic Acid Residue ◽  
Elastic Fibers ◽  
Transdermal Administration ◽  
Sialidase Activity ◽  
Fiber Assembly ◽  
Senescence Accelerated Mice

AbstractReduction of elastin in the skin causes various skin diseases as well as wrinkles and sagging with aging. Sialidase is a hydrolase that cleaves a sialic acid residue from sialoglycoconjugate. Cleavage of sialic acid from microfibrils by the sialidase isozyme Neu1 facilitates elastic fiber assembly. In the present study, we showed that a lower layer of the dermis and muscle showed relatively intense sialidase activity. The sialidase activity in the skin decreased with aging. Choline and geranate (CAGE), one of the ionic liquids, can deliver the sialidase subcutaneously while maintaining the enzymatic activity. The elastin level in the dermis was increased by applying sialidase from Arthrobacter ureafaciens (AUSA) with CAGE on the skin for 5 days in rats and senescence-accelerated mice prone 1 and 8. Sialidase activity in the dermis was considered to be mainly due to Neu2 based on the expression level of sialidase isozyme mRNA. Transdermal administration of Neu2 with CAGE also increased the level of elastin in the dermis. Therefore, not only Neu1 but also Neu2 would be involved in elastic fiber assembly. Transdermal administration of sialidase is expected to be useful for improvement of wrinkles and skin disorders due to the loss of elastic fibers.

scholarly journals A Negatively Charged Residue Stabilizes the Tropoelastin N-terminal Region for Elastic Fiber Assembly

2014 ◽  
Vol 289 (50) ◽  
pp. 34815-34826 ◽  
Author(s):  
Giselle C. Yeo ◽  
Clair Baldock ◽  
Steven G. Wise ◽  
Anthony S. Weiss
Keyword(s):  
Elastic Fiber ◽  
Terminal Region ◽  
Fiber Assembly ◽  
Charged Residue

Immunolocalization of 67 kDa elastin-binding protein in perinatal rat lungs

1992 ◽  
Vol 268 (2) ◽  
pp. 277-281 ◽  
Author(s):  
Kojiro Wasano ◽  
Yasuhiro Hirakawa ◽  
Keiichiro Nakamura
Keyword(s):  
Binding Protein ◽  
Rat Lungs ◽  
Elastin Binding Protein

Recycling of the 67-kDa Elastin Binding Protein in Arterial Myocytes Is Imperative for Secretion of Tropoelastin

1995 ◽  
Vol 220 (2) ◽  
pp. 312-324 ◽  
Author(s):  
Aleksander Hinek ◽  
Fred W. Keeley ◽  
John Callahans
Keyword(s):  
Binding Protein ◽  
Elastin Binding Protein
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