Immunolocalization of 67 kDa elastin-binding protein in perinatal rat lungs

1992 ◽  
Vol 268 (2) ◽  
pp. 277-281 ◽  
Author(s):  
Kojiro Wasano ◽  
Yasuhiro Hirakawa ◽  
Keiichiro Nakamura
Keyword(s):  
Binding Protein ◽  
Rat Lungs ◽  
Elastin Binding Protein

Recycling of the 67-kDa Elastin Binding Protein in Arterial Myocytes Is Imperative for Secretion of Tropoelastin

1995 ◽  
Vol 220 (2) ◽  
pp. 312-324 ◽  
Author(s):  
Aleksander Hinek ◽  
Fred W. Keeley ◽  
John Callahans
Keyword(s):  
Binding Protein ◽  
Elastin Binding Protein

scholarly journals Interaction between the Elastin Peptide VGVAPG and Human Elastin Binding Protein

2012 ◽  
Vol 288 (2) ◽  
pp. 1317-1328 ◽  
Author(s):  
Charlotte Blanchevoye ◽  
Nicolas Floquet ◽  
Amandine Scandolera ◽  
Stéphanie Baud ◽  
Pascal Maurice ◽  
...  
Keyword(s):  
Binding Protein ◽  
Elastin Binding Protein

scholarly journals Primary and Secondary Elastin-Binding Protein Defect Leads to Impaired Elastogenesis in Fibroblasts from GM1-Gangliosidosis Patients

2005 ◽  
Vol 167 (6) ◽  
pp. 1689-1698 ◽  
Author(s):  
Anna Caciotti ◽  
Maria Alice Donati ◽  
Tiziana Bardelli ◽  
Alessandra d'Azzo ◽  
Graziella Massai ◽  
...  
Keyword(s):  
Binding Protein ◽  
Gm1 Gangliosidosis ◽  
Elastin Binding Protein

scholarly journals The subcellular localization of the β-galactoside-binding protein of rat lung

1982 ◽  
Vol 204 (1) ◽  
pp. 97-102 ◽  
Author(s):  
G L Sanford ◽  
L D Davis ◽  
J T Powell
Keyword(s):  
Subcellular Localization ◽  
Immunoglobulin G ◽  
Specific Binding ◽  
Binding Protein ◽  
Rat Lung ◽  
Lectin Activity ◽  
Intracellular Location ◽  
Immature Rat ◽  
Adult Rat ◽  
Rat Lungs

The subcellular localization of the beta-galactoside-binding protein, or lectin, from rat lung was investigated by the specific binding of anti-lectin immunoglobulin G to subcellular fractions. We used both adult and immature (12-day-old) rats; the immature rat lungs have an 8-10-fold greater concentration than adult rat lungs [Powell & Whitney (1980) Biochem. J. 188, 1-8]. In both groups of animals we observed greater specific binding of anti-lectin immunoglobulin G to intracellular membrane (mitochondrial and microsomal fractions) than to plasma membranes. Pre-incubation of membrane fractions with lactose resulted in a marked diminution of anti-lectin immunoglobulin G binding. In the adult rat lung most (approx. 80%) of the lectin activity was membrane-associated. In the immature rat lung only approx. 30% of the lectin activity was membrane associated and most of the beta-galactoside-binding protein appeared to be a soluble cytoplasmic component. The rat lung beta-galactoside-binding protein appeared to have a broad but predominantly intracellular location, being associated with membranes through one of its galactoside-binding sites.

scholarly journals Tropoelastin Inhibits Vascular Calcification via 67-kDa Elastin Binding Protein in Cultured Bovine Aortic Smooth Muscle Cells

2004 ◽  
Vol 11 (3) ◽  
pp. 159-166 ◽  
Author(s):  
Hiroshi Wachi ◽  
Hideki Sugitani ◽  
Hayato Murata ◽  
Junji Nakazawa ◽  
Robert P. Mecham ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Calcification ◽  
Binding Protein ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Elastin Binding Protein

Fibulin-5 is an elastin-binding protein essential for elastic fibre development in vivo

Nature ◽  
2002 ◽  
Vol 415 (6868) ◽  
pp. 168-171 ◽  
Author(s):  
Hiromi Yanagisawa ◽  
Elaine C. Davis ◽  
Barry C. Starcher ◽  
Takashi Ouchi ◽  
Masashi Yanagisawa ◽  
...  
Keyword(s):  
Binding Protein ◽  
Elastic Fibre ◽  
Fibre Development ◽  
Elastin Binding Protein

scholarly journals 67-kD elastin-binding protein is a protective "companion" of extracellular insoluble elastin and intracellular tropoelastin.

1994 ◽  
Vol 126 (2) ◽  
pp. 563-574 ◽  
Author(s):  
A Hinek ◽  
M Rabinovitch
Keyword(s):  
Binding Protein ◽  
Light And Electron Microscopy ◽  
Serine Proteinase ◽  
Elastic Fibers ◽  
Fiber Assembly ◽  
Elastin Fiber ◽  
Alternatively Spliced ◽  
Elastin Binding Protein ◽  
Highly Hydrophobic

The 67-kD elastin-binding protein (EBP) mediates cell adhesion to elastin and elastin fiber assembly, and it is similar, if not identical, to the 67-kD enzymatically inactive, alternatively spliced beta-galactosidase. The latter contains an elastin binding domain (S-GAL) homologous both to the aorta EBP and to NH2-terminal sequences of serine proteinases (Hinek, A., M. Rabinovitch, F. W. Keeley, and J. Callahan. 1993. J. Clin. Invest. 91:1198-1205). We now confirm the functional importance of this homology by showing that elastolytic activity of a representative serine elastase, porcine pancreatic elastase, was prevented by an antibody (anti-S-GAL) and by competing with purified EBP or S-GAL peptide. Immunohistochemistry of adult aorta indicates that the EBP exists as a permanent component of mature elastic fibers. This observation, together with the in vitro studies, suggests that the EBP could protect insoluble elastin from extracellular proteolysis and contribute to the extraordinary stability of this protein. Double immunolabeling of fetal lamb aorta with anti-S-GAL and antitropoelastin antibodies demonstrated, under light and electron microscopy, intracellular colocalization of the proteins in smooth muscle cells (SMC). Incubation of SMC with galactosugars to dissociate tropoelastin from EBP caused intracellular aggregation of tropoelastin. A tropoelastin/EBP complex was extracted from SMC lysates by coimmunoprecipitation and cross-linking, and its functional significance was addressed by showing that its dissociation by galactosugars caused degradation of tropoelastin by endogenous serine proteinase(s). This suggests that the EBP may also serve as a "companion" to intracellular tropoelastin, protecting this highly hydrophobic protein from self-aggregation and proteolytic degradation.

scholarly journals Normal Wound Healing in Mice Deficient for Fibulin-5, an Elastin Binding Protein Essential for Dermal Elastic Fiber Assembly

2006 ◽  
Vol 126 (12) ◽  
pp. 2707-2714 ◽  
Author(s):  
Qian Zheng ◽  
Jiwon Choi ◽  
Leonie Rouleau ◽  
Richard L. Leask ◽  
James A. Richardson ◽  
...  
Keyword(s):  
Wound Healing ◽  
Binding Protein ◽  
Elastic Fiber ◽  
Fiber Assembly ◽  
Normal Wound Healing ◽  
Elastin Binding Protein

Elastin Binding Protein and FKBP65 Modulatein VitroSelf-Assembly of Human Tropoelastin

Biochemistry ◽  
2013 ◽  
Vol 52 (44) ◽  
pp. 7731-7741 ◽  
Author(s):  
Ming Miao ◽  
Sean E. Reichheld ◽  
Lisa D. Muiznieks ◽  
Yayi Huang ◽  
Fred W. Keeley
Keyword(s):  
Binding Protein ◽  
Elastin Binding Protein

The staphylococcal elastin-binding protein regulates zinc-dependent growth/biofilm formation

2014 ◽  
Vol 156 (3) ◽  
pp. 155-162 ◽  
Author(s):  
Makoto Nakakido ◽  
Chihiro Aikawa ◽  
Ichiro Nakagawa ◽  
Kouhei Tsumoto
Keyword(s):  
Biofilm Formation ◽  
Binding Protein ◽  
Elastin Binding Protein ◽  
Dependent Growth
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