Development of Auxotrophic Agrobacterium tumefaciens for Gene Transfer in Plant Tissue Culture

2004 ◽  
Vol 20 (3) ◽  
pp. 890-896 ◽  
Author(s):  
J.I. Collens ◽  
D.R. Lee ◽  
A.M. Seeman ◽  
W.R. Curtis
Keyword(s):  
Tissue Culture ◽  
Agrobacterium Tumefaciens ◽  
Gene Transfer ◽  
Plant Tissue Culture ◽  
Plant Tissue

scholarly journals Effects of Different β-Lactam Antibiotics on Indirect Tomato (Solanum lycopersicum L.) Shoot Organogenesis and Agrobacterium tumefaciens Growth Inhibition In Vitro

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 660
Author(s):  
Nataliya V. Varlamova ◽  
Yuliya I. Dolgikh ◽  
Andrey O. Blinkov ◽  
Ekaterina N. Baranova ◽  
Marat R. Khaliluev
Keyword(s):  
Tissue Culture ◽  
Agrobacterium Tumefaciens ◽  
Growth Inhibition ◽  
Clavulanic Acid ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Shoot Organogenesis ◽  
Disk Diffusion Assay ◽  
Diffusion Assay

A β-lactams that act by inhibiting the bacterial cell wall biosynthesis are one of the most common classes of antibiotics applied to suppress the growth of latent bacterial infection associated with the plant tissue culture, as well as in the Agrobacterium-mediated transformation techniques. Plant sensitivity to antibiotics usually is species-, genotype-, or even tissue-specific and mainly depends on concentrations, growth conditions, and culture system. In the presented article, we estimated a comparative effect of four β-lactam antibiotics (Claforan®, timentin, amoxicillin, and Amoxiclav®) at different concentrations in an agar-solidified Murashige and Skoog (MS) culture medium supplemented with 5 mg L−1 6-benzylaminopurine (6-BA) and 0.1 mg L−1 indole-3-acetic acid (IAA) on in vitro callus induction and shoot organogenesis from hypocotyl and cotyledon explants of two tomato cultivars (Rekordsmen, Moryana). The role of clavulanic acid in combination with amoxicillin (Amoxiclav®) in the shoot organogenesis frequency and number of shoots per explant has been demonstrated. Additionally, the growth inhibition of Agrobacterium tumefaciens AGL0 strain according to agar disk-diffusion assay was studied. As a result, both stimulatory (timentin, amoxicillin, and Amoxiclav®) and inhibitory (Claforan®) effects of β-lactam antibiotics on in vitro morphogenetic responses of tomato were noted. It was found that clavulanic acid, which is part of the commercial antibiotic Amoxiclav®, significantly increased the shoot regeneration frequency from cotyledon and hypocotyl explants of Rekordsmen tomato cultivar. Possible reasons for the stimulating effect of clavulanic acid on the induction of shoot organogenesis are discussed. According to agar disk-diffusion assay, the maximum diameter of growth inhibition zones (43.9 mm) was identified using 200 mg L−1 timentin. The in vitro antibacterial activity of tested β-lactam antibiotics was arranged in the following order: timentin > Claforan® > amoxicillin ≥ Amoxiclav®. Thus, to suppress the growth of internal and latent bacterial infection of tomato plant tissue culture, as well as for transformation of Moryana and Rekordsmen cultivars by A. tumefaciens strain AGL0, we recommend adding of 100–200 mg L−1 timentin or 400–800 mg L−1 Amoxiclav® to the shoot induction medium.

Development of Plant Tissue Culture Profile of Dioscorea deltoidea

Planta Medica ◽  
2013 ◽  
Vol 79 (05) ◽  
Author(s):  
M Mujeeb ◽  
M Amir ◽  
AS Nadeem ◽  
M Aqil ◽  
AK Najmi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Dioscorea Deltoidea

Media derived from brown seaweeds Cystoseira myriophylloides and Fucus spiralis for in vitro plant tissue culture

2016 ◽  
Vol 128 (2) ◽  
pp. 437-446 ◽  
Author(s):  
Siham Esserti ◽  
Mohamed Faize ◽  
Lalla Aicha Rifai ◽  
Amal Smaili ◽  
Malika Belfaiza ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Brown Seaweeds ◽  
Fucus Spiralis ◽  
In Vitro Plant

Plant Tissue Culture

1981 ◽  
Vol 43 (9) ◽  
pp. 508-512
Author(s):  
Patricia Pietropaolo
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue
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