EFFECT OF DIFFERENT TREATMENTS ON REGENERATION, GROWTH ANDACCLIMATIZATION OF PINEAPPLE  PLANTLETS PRODUCED BY PLANT TISSUE CULTURE 1-MICROPROPAGATION VIA ADVENTITIOUS SHOOT REGENERATION FROM LEAVES SEGEMENTS CULTURE OF Ananas comosus .L.Merr.cv.Del Mont

Abstract Background A frequent problem associated with the tissue culture of Compositae species such as chicory (Cichorium intybus L.) and lettuce (Lactuca sativa L.) is the premature bolting to in vitro flowering of regenerated plants. Plants exhibiting such phase changes have poor survival and poor seed set upon transfer from tissue culture to greenhouse conditions. This can result in the loss of valuable plant lines following applications of cell and tissue culture for genetic manipulation. Results This study demonstrates that chicory and lettuce plants exhibiting stable in vitro flowering can be rejuvenated by a further cycle of adventitious shoot regeneration from cauline leaves. The resulting rejuvenated plants exhibit substantially improved performance following transfer to greenhouse conditions, with increased frequency of plant survival, a doubling of the frequency of plants that flowered, and substantially increased seed production. Conclusion As soon as in vitro flowering is observed in unique highly-valued chicory and lettuce lines, a further cycle of adventitious shoot regeneration from cauline leaves should be implemented to induce rejuvenation. This re-establishes a juvenile phase accompanied by in vitro rosette formation, resulting in substantially improved survival, flowering and seed set in a greenhouse, thereby ensuring the recovery of future generations from lines genetically manipulated in cell and tissue culture.

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Plant Preservative Mixture™ Can Affect Shoot Regeneration from Leaf Explants of Chrysanthemum, European Birch, and Rhododendron

HortScience ◽

10.21273/hortsci.36.4.768 ◽

2001 ◽

Vol 36 (4) ◽

pp. 768-769 ◽

Cited By ~ 13

Author(s):

Mary W. George ◽

Robert R. Tripepi

Keyword(s):

Tissue Culture ◽

Shoot Regeneration ◽

Broad Spectrum ◽

Plant Tissue Culture ◽

Plant Tissue ◽

Betula Pendula ◽

Leaf Explants ◽

Conventional Antibiotics ◽

Number Of Shoots

Plant Preservative Mixture™ (PPM), a relatively new, broad-spectrum preservative and biocide for use in plant tissue culture, was evaluated as an alternative to the use of conventional antibiotics and fungicides in plant tissue culture. Concentrations of 0.5 to 4.0 mL·L-1 were tested with leaf explants of chrysanthemum (Dendranthem×grandiflora Kitam), European birch (Betula pendula Roth), and rhododendron (Rhododendron catawbiense Michx.). PPM had little effect on the percentage of explants forming shoots and the number of shoots formed per explant in birch and rhododendron, but dramatically reduced both responses in chrysanthemum. Therefore, the effects of PPM must be evaluated for each species of interest prior to use.

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Effect of Plant Preservative Mixture Ppmtm on the Shoot Regeneration of Watercress (Nasturtium Officinale)

Science Journal of University of Zakho ◽

10.25271/2017.5.2.366 ◽

2017 ◽

Vol 5 (2) ◽

pp. 187

Author(s):

Hadar Saeed Faizy ◽

Sami Rishak AL-Zubaydi ◽

Muraleedharan Nair

Keyword(s):

Tissue Culture ◽

Shoot Regeneration ◽

Plant Tissue Culture ◽

Plant Tissue ◽

Dry Weight ◽

Single Node ◽

Nasturtium Officinale ◽

Apical Buds ◽

Pair Number ◽

Conventional Antibiotics

Plant Preservative Mixture™ (PPM), a relatively new broad-spectrum preservative and biocide for use in plant tissue culture, was evaluated as an alternative to the use of conventional antibiotics and fungicides in plant tissue culture. Culture inoculated in MS media supplemented with PPM (1.5 ml/l) was the effective concentration which gave the best values. The top values were recorded for all studied characters using apical buds compared with lateral buds. The combination between apical buds and (1.5 ml/l ) PPM concentration showed the superior values of all studied parameters( 33.25%,19.40%, 14.46%,20.53% and 79.46%)(60.55%,39.80%,20.97%,45.33% and 54.44%) and (31.20%, 20. 06%, 12.33%, 35.13% and 81.06 %) for contamination, %bacterial contamination, % offungi contamination, dead explants% and survival explants% respectively. Different concentrations of PPM (0, 0.5, 1.0, 1.5, 2.0 and 2.5 ml/l) were tested using single node and apex explants of watercress (Nasturtium officinale). PPM at (1.5ml/l) had significant effect on the studied characters; shoots height, shoots number, leaf pairs number, fresh and dry weight which they reaches (4.16, 4.62, 42.00,0.524 and 0.063 g, respectively ). Apex bud explants showed the greatest effect on shoots height shoots number, leaf pairs number, fresh and dry weight and their values were 3.52, 32.02, 3.38, 0.405 and 0.036 g, respectively. The best parameter were recorded on MS media supplemented with PPM at (1.5ml/l) with apex buds explant (4.55, 5.34, 46.28, 0.570 and 0.085, respectively) for shoots height, shoots number, leaf pair number, fresh and dry weight. Current study aimed to determine the best concentration of PPM for limiting the contamination of watercress and micro shoot regeneration.

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Tissue culture of Cecropia glaziovii Sneth (urticaceae): vegetative micropropagation and plant regeneration from callus

Ciência e Agrotecnologia ◽

10.1590/s1413-70542010000500024 ◽

2010 ◽

Vol 34 (5) ◽

pp. 1245-1252 ◽

Cited By ~ 1

Author(s):

Marcos Nopper Alves

Keyword(s):

Tissue Culture ◽

Acetic Acid ◽

Shoot Regeneration ◽

Culture Media ◽

Adventitious Shoot ◽

Naphthalene Acetic Acid ◽

Ms Medium ◽

Adventitious Shoot Regeneration ◽

Popular Medicine ◽

Superior Genotype

Cecropia glaziovii is a tree with used in Brazilian popular medicine. Methods allowing the clonal propagation of this species are of great interest for superior genotype multiplication and perpetuation. For this reason, we examined the effect of different culture media and different types of explants on adventitious shoot regeneration from callus and buds of C. glaziovii. Leaves, petioles and stipules obtained from aseptically grown seedlings or from pre-sterilized plants were used to initiate cultures. Adventitious shoot regeneration was achieved when apical and axillary buds were inoculated on gelled Murashige & Skoog (MS) medium supplemented with 6-benzylaminopurine alone (BAP) (1.0, 5.0 or 10.0 mg L-1) or combined with -naphthalene acetic acid (NAA) (1.0 or 2.0 mg L-1), after 40 days of culture. Best callus production was obtained after 30 days of petioles' culture on gelled MS medium with 2,4 dichlorophenoxyacetic acid (2,4-D) (5.0 mg L-1) combined with BAP (1.0 mg L-1). Successful shoot regeneration from callus was achieved when MS medium supplemented with zeatin (ZEA) (0.1 mg L-1) alone or combined with 2,4-D (1.0 or 5.0 mg L-1) was inoculated with friable callus obtained from petioles. All shoots were rooted by inoculation on MS medium supplemented with indole-3-acetic acid (IAA) (1.0 mg L-1). Rooted plants transferred to potting soil were successfully established. All in vitro regenerated plantlets showed to be normal, without morphological variations, being also identical to the source plant. Our study has shown that C. glaziovii can be propagated by tissue culture methods, allowing large scale multiplication of superior plants for pharmacological purposes.

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