scholarly journals Effects of Different β-Lactam Antibiotics on Indirect Tomato (Solanum lycopersicum L.) Shoot Organogenesis and Agrobacterium tumefaciens Growth Inhibition In Vitro

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 660
Author(s):  
Nataliya V. Varlamova ◽  
Yuliya I. Dolgikh ◽  
Andrey O. Blinkov ◽  
Ekaterina N. Baranova ◽  
Marat R. Khaliluev
Keyword(s):  
Tissue Culture ◽  
Agrobacterium Tumefaciens ◽  
Growth Inhibition ◽  
Clavulanic Acid ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Shoot Organogenesis ◽  
Disk Diffusion Assay ◽  
Diffusion Assay

A β-lactams that act by inhibiting the bacterial cell wall biosynthesis are one of the most common classes of antibiotics applied to suppress the growth of latent bacterial infection associated with the plant tissue culture, as well as in the Agrobacterium-mediated transformation techniques. Plant sensitivity to antibiotics usually is species-, genotype-, or even tissue-specific and mainly depends on concentrations, growth conditions, and culture system. In the presented article, we estimated a comparative effect of four β-lactam antibiotics (Claforan®, timentin, amoxicillin, and Amoxiclav®) at different concentrations in an agar-solidified Murashige and Skoog (MS) culture medium supplemented with 5 mg L−1 6-benzylaminopurine (6-BA) and 0.1 mg L−1 indole-3-acetic acid (IAA) on in vitro callus induction and shoot organogenesis from hypocotyl and cotyledon explants of two tomato cultivars (Rekordsmen, Moryana). The role of clavulanic acid in combination with amoxicillin (Amoxiclav®) in the shoot organogenesis frequency and number of shoots per explant has been demonstrated. Additionally, the growth inhibition of Agrobacterium tumefaciens AGL0 strain according to agar disk-diffusion assay was studied. As a result, both stimulatory (timentin, amoxicillin, and Amoxiclav®) and inhibitory (Claforan®) effects of β-lactam antibiotics on in vitro morphogenetic responses of tomato were noted. It was found that clavulanic acid, which is part of the commercial antibiotic Amoxiclav®, significantly increased the shoot regeneration frequency from cotyledon and hypocotyl explants of Rekordsmen tomato cultivar. Possible reasons for the stimulating effect of clavulanic acid on the induction of shoot organogenesis are discussed. According to agar disk-diffusion assay, the maximum diameter of growth inhibition zones (43.9 mm) was identified using 200 mg L−1 timentin. The in vitro antibacterial activity of tested β-lactam antibiotics was arranged in the following order: timentin > Claforan® > amoxicillin ≥ Amoxiclav®. Thus, to suppress the growth of internal and latent bacterial infection of tomato plant tissue culture, as well as for transformation of Moryana and Rekordsmen cultivars by A. tumefaciens strain AGL0, we recommend adding of 100–200 mg L−1 timentin or 400–800 mg L−1 Amoxiclav® to the shoot induction medium.

Media derived from brown seaweeds Cystoseira myriophylloides and Fucus spiralis for in vitro plant tissue culture

2016 ◽  
Vol 128 (2) ◽  
pp. 437-446 ◽  
Author(s):  
Siham Esserti ◽  
Mohamed Faize ◽  
Lalla Aicha Rifai ◽  
Amal Smaili ◽  
Malika Belfaiza ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Brown Seaweeds ◽  
Fucus Spiralis ◽  
In Vitro Plant

scholarly journals In vitro plant tissue culture: means for production of biological active compounds

Planta ◽  
2018 ◽  
Vol 248 (1) ◽  
pp. 1-18 ◽  
Author(s):  
Claudia A. Espinosa-Leal ◽  
César A. Puente-Garza ◽  
Silverio García-Lara
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Active Compounds ◽  
In Vitro Plant

scholarly journals Endogenous Bacterial Contamination of Plant Tissue Culture Materials: Identification and Control Strategy

2018 ◽  
Vol 28 (1) ◽  
pp. 99-108 ◽  
Author(s):  
Mohammad Ali ◽  
Shefali Boonerjee ◽  
Mohammad Nurul Islam ◽  
Mihir Lal Saha ◽  
M Imdadul Hoque ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Bacterial Contamination ◽  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
Bacterial Isolates ◽  
Culture And Sensitivity ◽  
And Control

The endogenous bacterial contamination of plant tissue culture materials and their possible control was studied. Nine bacterial isolates were isolated from the contaminated tissue culture materials viz. potato and tea. On the basis of morphology and biochemical characters of nine isolates, seven were identified as Gram positive belonging to Bacillus alcalophilus, B. circulans, B. infantis, B. lentus, B. schlegelii, B. pumilus and B. subtilis. Remaining two were Gram negative and identified as Enterobacter cloacae sub. sp. dissolvens and Pantoea agglomerans. Molecular analysis was conducted on the basis of 16S rDNA sequence to confirm three isolates. Culture and sensitivity test was carried out to screen out the antibiotic sensitivity where streptomycin (S-10), polymyxin (PB-300) and gentamicin (CN-120) antibiotics were found to be effective against all bacterial isolates. The culture and sensitivity test reflected the feasibility to control or eliminate the contaminant bacteria during in vitro culture of plant which is very much required in the commercial tissue culture production.Plant Tissue Cult. & Biotech. 28(1): 99-108, 2018 (June)

Development of Auxotrophic Agrobacterium tumefaciens for Gene Transfer in Plant Tissue Culture

2004 ◽  
Vol 20 (3) ◽  
pp. 890-896 ◽  
Author(s):  
J.I. Collens ◽  
D.R. Lee ◽  
A.M. Seeman ◽  
W.R. Curtis
Keyword(s):  
Tissue Culture ◽  
Agrobacterium Tumefaciens ◽  
Gene Transfer ◽  
Plant Tissue Culture ◽  
Plant Tissue

scholarly journals In vitro production of capsaicin through plant tissue culture

2017 ◽  
pp. 24-33
Author(s):  
Swetnisha, Ajitabh Bora, H.K. Gogoi, P.S. Raju
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
In Vitro Production ◽  
Agricultural Produce ◽  
Medicinal Properties ◽  
Method Of Production ◽  
Culture Strategies ◽  
Vitro Production

Capsaicin, a secondary metabolite produced in capsicum, is in high demand in pharmaceutical industry because of its various medicinal properties. Currently, the supply of capsaicin depends upon its extraction from capsicum fruits. This limits the production of capsaicin as it depends upon agricultural produce. The current review has compiled information from various literature published on chemistry and importance of capsaicin along with its method of production. It also reviews the process of in vitro production of capsaicin through plant tissue culture, strategies of increasing capsaicin accumulation and its advantages over extraction from fruits and artificial synthesis.

scholarly journals Sodium Dichloroisocyanurate: An eco-friendly chemical alternative for media autoclaving and explant sterilisation in plant tissue culture

2021 ◽  
Vol 12 (1) ◽  
pp. 107-112
Author(s):  
Simran Chandrahas Shetty ◽  
Narasimhan S
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Active Agent ◽  
Electrical Energy ◽  
Life Forms ◽  
Water Soluble ◽  
Viable Alternative ◽  
Sodium Dichloroisocyanurate

Autoclaving nutrient media is still considered as the optimum mode of sterilisation in plant cell and tissue culture. During the process steam under high pressure is maintained at 120 degrees Celsius, 15 psi for 15-20 minutes in a chamber, optimised to kill all possible microbial life forms. But the disadvantages related to the process of autoclaving are plentiful. They are, decrease in the media pH, salt precipitation, agar depolymerisation, carbohydrate hydrolysis, volatile obliteration and necessity of the infrastructure investment. Requirements of additional resources (time, human resources, electrical energy) have forced the lookout for a more viable alternative, that is, chemical sterilisation. The use of Sodium dichloroisocyanurate (NaDCC) is a useful alternative for media and explant sterilisation. NaDCC is stable, water-soluble, non-toxic and easy to use at room temperature, does not have any environmental hazards and is not phytotoxic. The use of NaDCC as a disinfectant has been documented well concerning water sterilisation, surface sterilisation and also as a broad spectrum disinfecting agent. Disinfecting property of NaDCC is due to the hydrolytic release of chlorine, and this can be utilised for sterilisation of media and explants in plant tissue culture. NaDCC is a useful alternative for autoclaving at a concentration range of 0.05 to 1.0 g/l. However, only a few reports are available for its use as a sterilising agent for media and explants for in vitro cultures of plants. This paper discusses and reviews the possibility of establishing NaDCC as an active agent for explant sterilisation and as a viable alternative to medium sterilisation through autoclaving.

scholarly journals IN VITRO SHOOT MULTIPLICATION OF STEVIA REBAUDIANA (BERT) THROUGH PLANT TISSUE CULTURE.

2016 ◽  
Vol 4 (11) ◽  
pp. 2300-2307
Author(s):  
Vibha Bhingradiya ◽  
◽  
Archana Mankad ◽  
Ruby Patel ◽  
Shivangi Mathur ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Stevia Rebaudiana ◽  
Shoot Multiplication

scholarly journals Plant Tissue Culture- A New Tool for Vegetable Improvement (Indian scenario): A Review

2021 ◽  
Author(s):  
Priyanka Bijalwan ◽  
Shilpa .
Keyword(s):  
Tissue Culture ◽  
Embryo Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Plant Cells ◽  
Meristem Culture ◽  
Culture Techniques ◽  
Short Period ◽  
Plant Sciences

In vitro culture of plant cells/tissues is now routine using a range of explant types from many of the important vegetable and fruit crops. Successful technologies include isolation, culture of tissues, cells, protoplasts, organs, embryos, ovules, anthers and microspores and regeneration from them of complete plantlets. The development of plant tissue culture technology represents one of the most exciting advances in plant sciences. For example, the prospect of being able to introduce, develop, produce, transfer and conserve the existing gene pool of plant sciences by using tissue culture methods opens up new opportunities for researches and entrepreneurs. The term plant tissue culture should denote in vitro cultivation of plant cells or tissues in an unorganized mass, i.e., callus culture. Plant tissue culture techniques, in combination with recombinant DNA technology, are the essential requirements for the development of transgenic plants. However, culture techniques like anther/pollen/ovule culture, meristem culture can themselves be utilized for crop improvement or may serve as an aid to conventional breeding. In recent, isolated microspore culture has developed as a breeding tool and an experimental system for various genetic manipulations. The inherent potentiality of a plant cell to give rise to a whole plant, a capacity which is often retained even after a cell has undergone final differentiation in the plant body, is described as ‘cellular totipotency’. On the other hand, production of virus-free plants via meristem culture can reduce losses caused by phyto-pathogens. Embryo culture has many potential uses ranging from overcoming seed dormancy to facilitation of inter-specific hybridization. Protoplast fusion technique can be used for the transfer of cytoplasmic male sterility from one species to another in a short period of time. In cabbage, male sterile cybrids are being utilized by seed companies to produce hybrid seeds on commercial scale and at competitive rates. Plant tissue culture and cell culture are providing useful methods for germplasm storage either by low temperature storage of organized tissue, or cryopreservation of cell or embryo culture.

scholarly journals In Vitro Micropropagation of Lilium candidum Bulb by Application of Multiple Hormone Concentrations Using Plant Tissue Culture Technique

2021 ◽  
Vol 8 (2) ◽  
pp. 244-253
Author(s):  
Akshay Milind Patil ◽  
Pooja Prakash Gunjal ◽  
Dr. Sonali Das
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Health Benefits ◽  
Vitamin B1 ◽  
Scar Tissue ◽  
Culture Technique ◽  
Therapeutic Uses ◽  
Micro Propagation

The multiplication efficacy by bulb is low and the plantlets are more susceptible to disease, therefore, there is a need to develop a protocol for its propagation. Lilium candidum is listed in the saitma prefecture Red Data Book as a critically endangered plant and rescuing information regarding its micro-propagation is rather limited. On this regard, the application of in vitro micropropagtion procedure might help to obtain large numbers of uniform plants of endangered species of Lilium. Dried lilies are a rich source of fiber and also rich in sodium and carbs. Lily bulbs have proteins and starch and also small quantities of iron, calcium, phosphorous, and vitamin B1, B2, C. The health benefits of the lily for the heart are well known on account of the active cardiac glycosides as well as the flavonoids which tend to stimulate the arteries and can cause them to dilute. Another one of the therapeutic uses of the lily flower is in the case of treating burns and preventing the formation of scar tissue. One of the main health benefits of the lily flower is that it helps regulating the heart rate there by allowing the heart to function more efficiently and regular. Having multiple medicinal properties we decided to cultivate Lilium candidum using plant tissue culture so farming can be increased using this cost efficient techniques. In this research, we have studied various Effect of different concentration of BAP and NAA on the initiation of Lilium candidum from bulb and IBA, IAA and NAA on the rooting of shoots of Lilium Candidum.

scholarly journals ISOLATION AND CHARACTERIZATION OF MDR BACTERIA FROM IN VITRO CULTURE OF BACOPA MONNIERA AND SUPPLEMENTATION OF NATURAL EXTRACTS TO CONTROL BACTERIAL CONTAMINATION

2016 ◽  
Vol 8 (11) ◽  
pp. 102
Author(s):  
Yash Sharma ◽  
Manish Bhardwaj ◽  
Anshita Nagar ◽  
Neeta Bhagat
Keyword(s):  
Tissue Culture ◽  
In Vitro Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Bacterial Contamination ◽  
Resistant Bacteria ◽  
Bacopa Monniera ◽  
Natural Extracts ◽  
Isolation And Characterization

Objective: The purpose of the present study was to isolate and characterize bacterial contamination from in vitro culture of Bacopa monniera callus culture.Methods: The two selected isolates (1 and 2) were identified by morphological, biochemical and molecular (16S rRNA gene sequencing) methods. Beside this antibiotic resistance was also determined.Results: The isolates were identified as closely related to Enterobacter cloacae (KU350623) (Isolate 1) and Myroides odoratimimus (KU382740) (Isolate 2). The isolate 1 and 2 were found to be resistant to streptomycin (25 mcg), dapsone (10 mcg), erythromycin (15 mcg), chloroamphenicol (25 mcg) and ampicillin (10 mcg). Supplementation of the natural extract was tested to control the contamination due to these multi drug resistant bacteria. Aqueous and alcoholic leaf extracts of Azadirachta indica was added to plant tissue culture media i.e. MS media in order to control contamination.Conclusion: The present studies suggest using natural extracts supplementation as a promising strategy to control the in vitro bacterial contamination in plant tissue culture.

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