Improved Xylan Hydrolysis of Corn Stover by Deacetylation with High Solids Dilute Acid Pretreatment

2011 ◽  
Vol 51 (1) ◽  
pp. 70-76 ◽  
Author(s):  
Xiaowen Chen ◽  
Joseph Shekiro ◽  
Rick Elander ◽  
Melvin Tucker
Keyword(s):  
Corn Stover ◽  
Dilute Acid Pretreatment ◽  
High Solids ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Xylan Hydrolysis ◽  
Hydrolysis Of

Dilute-Acid Pretreatment of Corn Stover Using a High-Solids Percolation Reactor

2004 ◽  
Vol 117 (2) ◽  
pp. 103-114 ◽  
Author(s):  
Yongming Zhu ◽  
Y. Y. Lee ◽  
Richard T. Elander
Keyword(s):  
Corn Stover ◽  
Dilute Acid Pretreatment ◽  
High Solids ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Percolation Reactor

scholarly journals Multiphysics modeling and simulation of high-solids dilute-acid pretreatment of corn stover in a steam-explosion reactor

2015 ◽  
Vol 268 ◽  
pp. 47-59 ◽  
Author(s):  
Hariswaran Sitaraman ◽  
Erik M. Kuhn ◽  
Ambarish Nag ◽  
Michael A. Sprague ◽  
Melvin P. Tucker ◽  
...  
Keyword(s):  
Modeling And Simulation ◽  
Corn Stover ◽  
Steam Explosion ◽  
Dilute Acid Pretreatment ◽  
High Solids ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Multiphysics Modeling

scholarly journals Investigation of interaction between cellulase and residual lignin fractions in acid-pretreated bamboo residues and their effect on enzymatic digestibility

2021 ◽  
Author(s):  
Wenqian Lin ◽  
Jinlai Yang ◽  
Yayue Zheng ◽  
Caoxing Huang ◽  
Qiang Yong
Keyword(s):  
Enzymatic Hydrolysis ◽  
Negative Impact ◽  
Interaction Mechanism ◽  
Dilute Acid Pretreatment ◽  
Affinity Constant ◽  
Residual Lignin ◽  
Dilute Acid ◽  
Enzymatic Digestibility ◽  
Acid Pretreatment ◽  
Hydrolysis Of

Abstract Background: During dilute acid pretreatment, pseudo lignin and lignin form droplets which deposit on the surface of lignocellulose, and further inhibit its enzymatic hydrolysis. However, how this lignin interacts with cellulase enzymes and then affects enzymatic hydrolysis is still unknown. In this work, different fractions of surface lignin (SL) obtained from dilute acid pretreated bamboo residues (DAP-BR) were extracted by various organic reagents and the residual lignin in extracted DAP-BR was obtained by milled wood lignin (MWL) method. All the obtained lignin fractions from DAP-BR were used to investigate the interaction mechanism between lignin and cellulase using surface plasmon resonance (SPR) technology in order to understand how they affect enzymatic hydrolysisResults: Results showed that removing surface lignin significantly decrease the enzymatic hydrolysis of DAP-BR from 36.5% to 18.6%. The addition of MWL samples to Avicel decreased enzymatic hydrolysis of Avicel, while different SL samples showed a slight increase to its enzymatic digestibility. Due to the higher molecular weight and hydrophobicity of MWL samples versus the SL samples, stronger affinity for MWL (KD = 6.8-24.7 nM) was found versus that of SL (KD = 39.4-52.6 nM) by SPR analysis. The affinity constant of all tested lignin had good correlations (R2>0.6) with their effects on enzymatic digestibility of extracted DAP-BR and Avicel.Conclusions: This work reveals that the surface lignin on DAP-BR is necessary towards maintaining enzyme digestibility levels, and its removal has a negative impact on the substrate’s digestibility.

scholarly journals Effect of Subsequent Dilute Acid and Enzymatic Hydrolysis on Reducing Sugar Production from Sugarcane Bagasse and Spent Citronella Biomass

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Robinson Timung ◽  
Narendra Naik Deshavath ◽  
Vaibhav V. Goud ◽  
Venkata V. Dasu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Crystallinity Index ◽  
Sem Analysis ◽  
Reducing Sugar ◽  
Dilute Acid Pretreatment ◽  
Crystalline Cellulose ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Hydrolysis Of

This work was aimed at investigating the effect of process parameters on dilute acid pretreatment and enzymatic hydrolysis of spent citronella biomass (after citronella oil extraction) and sugarcane bagasse on total reducing sugar (TRS) yield. In acid pretreatment, the parameters studied were acid concentration, temperature, and time. At the optimized condition (0.1 M H2SO4, 120°C, and 120 min), maximum TRS obtained was 452.27 mg·g−1and 487.50 mg·g−1for bagasse and citronella, respectively. Enzymatic hydrolysis of the pretreated biomass usingTrichoderma reesei26291 showed maximum TRS yield of 226.99 mg·g−1for citronella and 282.85 mg·g−1for bagasse at 10 FPU, 50°C, and 48 hr. The maximum crystallinity index (CI) of bagasse and citronella after acid pretreatment obtained from X-ray diffraction analysis was 64.41% and 56.18%, respectively. Decreased CI after enzymatic hydrolysis process to 37.28% and 34.16% for bagasse and citronella, respectively, revealed effective conversion of crystalline cellulose to glucose. SEM analysis of the untreated and treated biomass revealed significant hydrolysis of holocellulose and disruption of lignin.

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