Supramolecular catalysis of phosphoryl transfer: cocatalysis of pyrophosphate synthesis from acetyl phosphate mediated by macrocyclic polyamines

1987 ◽  
Vol 109 (23) ◽  
pp. 7047-7058 ◽  
Author(s):  
Mir Wais Hosseini ◽  
Jean Marie Lehn
Keyword(s):  
Phosphoryl Transfer ◽  
Acetyl Phosphate ◽  
Supramolecular Catalysis ◽  
Macrocyclic Polyamines

Supramolecular catalysis of H/D exchange in pyruvate by macrocyclic polyamines involving a reactive iminium intermediate

1997 ◽  
pp. 2073-2081 ◽  
Author(s):  
Hicham Fenniri ◽  
Carol Dallaire ◽  
Daniel P. Funeriu ◽  
Jean-Marie Lehn
Keyword(s):  
Supramolecular Catalysis ◽  
Macrocyclic Polyamines

Supramolecular Catalysis of H/D Exchange in Malonate Ions by Macrocyclic Polyamines: A Model Enzyme with Enolase Activity

1996 ◽  
Vol 35 (3) ◽  
pp. 337-339 ◽  
Author(s):  
Hicham Fenniri ◽  
Jean-Marie Lehn ◽  
Annie Marquis-Rigault
Keyword(s):  
Supramolecular Catalysis ◽  
Macrocyclic Polyamines

Supramolecular Catalysis of Phosphoryl Transfer

1993 ◽  
Vol 75 (1-4) ◽  
pp. 1-1
Author(s):  
Jean-Marie Lehn
Keyword(s):  
Phosphoryl Transfer ◽  
Supramolecular Catalysis

scholarly journals Novel Pyrophosphate-Forming Acetate Kinase from the Protist Entamoeba histolytica

2012 ◽  
Vol 11 (10) ◽  
pp. 1249-1256 ◽  
Author(s):  
Matthew L. Fowler ◽  
Cheryl Ingram-Smith ◽  
Kerry S. Smith
Keyword(s):  
Entamoeba Histolytica ◽  
Acetate Kinase ◽  
Phosphoryl Group ◽  
Phosphoryl Transfer ◽  
Acetyl Phosphate ◽  
Phosphate Binding ◽  
Methanosarcina Thermophila ◽  
Inorganic Pyrophosphate ◽  
Content Type ◽  
Cell Extracts

ABSTRACTAcetate kinase (ACK) catalyzes the reversible synthesis of acetyl phosphate by transfer of the γ-phosphate of ATP to acetate. Here we report the first biochemical and kinetic characterization of a eukaryotic ACK, that from the protistEntamoeba histolytica. Our characterization revealed that this protist ACK is the only known member of the ASKHA structural superfamily, which includes acetate kinase, hexokinase, and other sugar kinases, to utilize inorganic pyrophosphate (PPi)/inorganic phosphate (Pi) as the sole phosphoryl donor/acceptor. Detection of ACK activity inE. histolyticacell extracts in the direction of acetate/PPiformation but not in the direction of acetyl phosphate/Piformation suggests that the physiological direction of the reaction is toward acetate/PPiproduction. Kinetic parameters determined for each direction of the reaction are consistent with this observation. TheE. histolyticaPPi-forming ACK follows a sequential mechanism, supporting a direct in-line phosphoryl transfer mechanism as previously reported for the well-characterizedMethanosarcina thermophilaATP-dependent ACK. Characterizations of enzyme variants altered in the putative acetate/acetyl phosphate binding pocket suggested that acetyl phosphate binding is not mediated solely through a hydrophobic interaction but also through the phosphoryl group, as for theM. thermophilaACK. However, there are key differences in the roles of certain active site residues between the two enzymes. The absence of known ACK partner enzymes raises the possibility that ACK is part of a novel pathway inEntamoeba.

ChemInform Abstract: Supramolecular Catalysis in the Hydrolysis of ATP Facilitated by Macrocyclic Polyamines: Mechanistic Studies.

ChemInform ◽  
1987 ◽  
Vol 18 (22) ◽  
Author(s):  
M. W. HOSSEINI ◽  
J.-M. LEHN ◽  
L. MAGGIORA ◽  
K. B. MERTES ◽  
M. P. MERTES
Keyword(s):  
Mechanistic Studies ◽  
Supramolecular Catalysis ◽  
Macrocyclic Polyamines ◽  
Hydrolysis Of

Supramolecular catalysis: polyammonium macrocycles as enzyme mimics for phosphoryl transfer in ATP hydrolysis

1989 ◽  
Vol 111 (16) ◽  
pp. 6330-6335 ◽  
Author(s):  
Mir Wais Hosseini ◽  
Jean Marie Lehn ◽  
Kenneth C. Jones ◽  
Kathleen E. Plute ◽  
Kristin Bowman Mertes ◽  
...  
Keyword(s):  
Atp Hydrolysis ◽  
Phosphoryl Transfer ◽  
Enzyme Mimics ◽  
Supramolecular Catalysis

scholarly journals The Intracellular Concentration of Acetyl Phosphate in Escherichia coli Is Sufficient for Direct Phosphorylation of Two-Component Response Regulators

2007 ◽  
Vol 189 (15) ◽  
pp. 5574-5581 ◽  
Author(s):  
Adam H. Klein ◽  
Ana Shulla ◽  
Sylvia A. Reimann ◽  
David H. Keating ◽  
Alan J. Wolfe
Keyword(s):  
Escherichia Coli ◽  
Intracellular Concentration ◽  
Phosphoryl Transfer ◽  
Acetyl Phosphate ◽  
Wild Type ◽  
Response Regulators ◽  
Acetyl Coenzyme A ◽  
Global Signal ◽  
Two Component ◽  
Layer Chromatography

ABSTRACT Acetyl phosphate, the intermediate of the AckA-Pta pathway, acts as a global signal in Escherichia coli. Although acetyl phosphate clearly signals through two-component response regulators, it remains unclear whether acetyl phosphate acts as a direct phospho donor or functions through an indirect mechanism. We used two-dimensional thin-layer chromatography to measure the relative concentrations of acetyl phosphate, acetyl coenzyme A, ATP, and GTP over the course of the entire growth curve. We estimated that the intracellular concentration of acetyl phosphate in wild-type cells reaches at least 3 mM, a concentration sufficient to activate two-component response regulators via direct phosphoryl transfer.

ChemInform Abstract: Supramolecular Catalysis: Polyammonium Macrocycles as Enzyme Mimics for Phosphoryl Transfer in ATP Hydrolysis.

ChemInform ◽  
1989 ◽  
Vol 20 (45) ◽  
Author(s):  
M. W. HOSSEINI ◽  
J.-M. LEHN ◽  
K. C. JONES ◽  
K. E. PLUTE ◽  
K. B. MERTES ◽  
...  
Keyword(s):  
Atp Hydrolysis ◽  
Phosphoryl Transfer ◽  
Enzyme Mimics ◽  
Supramolecular Catalysis

Supramolecular catalysis in the hydrolysis of ATP facilitated by macrocyclic polyamines: mechanistic studies

1987 ◽  
Vol 109 (2) ◽  
pp. 537-544 ◽  
Author(s):  
Mir Wais Hosseini ◽  
Jean Marie Lehn ◽  
Linda Maggiora ◽  
Kristin B. Mertes ◽  
Mathias P. Mertes
Keyword(s):  
Mechanistic Studies ◽  
Supramolecular Catalysis ◽  
Macrocyclic Polyamines ◽  
Hydrolysis Of
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