Characterization of an immobilized digestive enzyme system for determination of protein digestibility

1984 ◽  
Vol 32 (2) ◽  
pp. 334-339 ◽  
Author(s):  
David H. Porter ◽  
Harold E. Swaisgood ◽  
George L. Catignani
Keyword(s):  
Enzyme System ◽  
Digestive Enzyme ◽  
Protein Digestibility

scholarly journals ISOLATION, CHARACTERIZATION, AND IDENTIFICATION OF PROTEOLYTIC BACTERIA TO IMPROVE PROTEIN DIGESTIBILITY OF FISH FEED INGREDIENTSISOLATION, CHARACTERIZATION, AND IDENTIFICATION OF PROTEOLYTIC BACTERIA TO IMPROVE PROTEIN DIGESTIBILITY OF FISH FEED INGREDIENTS

2018 ◽  
Vol 13 (2) ◽  
Author(s):  
Muyasari Mulyasari ◽  
Mas Tri Djoko Sunarno ◽  
Lusi Herawati Suryaningrum
Keyword(s):  
Protease Activity ◽  
Hot Springs ◽  
Raw Materials ◽  
Protein Digestibility ◽  
Mangrove Sediment ◽  
Fish Feed ◽  
Proteolytic Bacteria ◽  
Shrimp Head

Protease is one of the potential enzymes used to improve the quality of local raw materials as fish feedstuff. The aim of this research was to find high protease activity-bacteria isolates in improving protein digestibility of fish feedstuff. Isolation was conducted on various growth substrates such as water and sediment of hot springs, mangrove sediment, and shrimp head waste. The parameters used in the selection and characterization of potential proteolytic bacteria were qualitative and quantitative tests of proteolytic activity. Microbes that have the highest enzyme activity were then selected and characterized based on their growth curve, production time of protease, and determination of optimum condition (temperature and pH) of protease activity. The result showed that there are only 18 isolates from 44 isolates positively had protease activity (qualitatively). Three isolates with the highest activity (RGL1.1, PL1.1, and PL1.2) qualitatively and quantitatively were then selected to be tested for their pathogenecity on tilapia. The results showed that only RGL1.1 was not pathogenic and therefore, potentially used as a protease producer candidate. The characterization of RGL1.1 isolates showed that the exponential phase of RGL1.1 was thirty-sixth hour in line with the optimum activity of enzyme production. The optimum pH and temperature of RGL1.1 isolate were 6 and 50°C, respectively. RGL1.1 isolate is a Gram-positive rod-shaped bacteria that has 99.53% of resemblance to Bacillus cereus.

scholarly journals ISOLATION, CHARACTERIZATION, AND IDENTIFICATION OF PROTEOLYTIC BACTERIA TO IMPROVE PROTEIN DIGESTIBILITY OF FISH FEED INGREDIENTSISOLATION, CHARACTERIZATION, AND IDENTIFICATION OF PROTEOLYTIC BACTERIA TO IMPROVE PROTEIN DIGESTIBILITY OF FISH FEED INGREDIENTS

2018 ◽  
Vol 13 (2) ◽  
pp. 83
Author(s):  
Muyasari Mulyasari ◽  
Mas Tri Djoko Sunarno ◽  
Lusi Herawati Suryaningrum
Keyword(s):  
Protease Activity ◽  
Hot Springs ◽  
Raw Materials ◽  
Protein Digestibility ◽  
Mangrove Sediment ◽  
Fish Feed ◽  
Proteolytic Bacteria ◽  
Shrimp Head

Protease is one of the potential enzymes used to improve the quality of local raw materials as fish feedstuff. The aim of this research was to find high protease activity-bacteria isolates in improving protein digestibility of fish feedstuff. Isolation was conducted on various growth substrates such as water and sediment of hot springs, mangrove sediment, and shrimp head waste. The parameters used in the selection and characterization of potential proteolytic bacteria were qualitative and quantitative tests of proteolytic activity. Microbes that have the highest enzyme activity were then selected and characterized based on their growth curve, production time of protease, and determination of optimum condition (temperature and pH) of protease activity. The result showed that there are only 18 isolates from 44 isolates positively had protease activity (qualitatively). Three isolates with the highest activity (RGL1.1, PL1.1, and PL1.2) qualitatively and quantitatively were then selected to be tested for their pathogenecity on tilapia. The results showed that only RGL1.1 was not pathogenic and therefore, potentially used as a protease producer candidate. The characterization of RGL1.1 isolates showed that the exponential phase of RGL1.1 was thirty-sixth hour in line with the optimum activity of enzyme production. The optimum pH and temperature of RGL1.1 isolate were 6 and 50°C, respectively. RGL1.1 isolate is a Gram-positive rod-shaped bacteria that has 99.53% of resemblance to Bacillus cereus.

Characterization of Impressed Current Technique to Model Corrosion of Reinforcement in Concrete

2020 ◽  
Vol 11 (1) ◽  
pp. 93-99
Author(s):  
Abu Zakir Morshed ◽  
Sheikh Shakib ◽  
Tanzim Jahin
Keyword(s):  
Immersion Time ◽  
Chloride Content ◽  
Engineering Science ◽  
Coastal Regions ◽  
Faraday’S Law ◽  
Current Technique ◽  
Corrosion Cell ◽  
Impressed Current

Corrosion of reinforcement is an important durability concern for the structures exposed to coastal regions. Since corrosion of reinforcement involves long periods of time, impressed current technique is usually used to accelerate the corrosion of reinforcement in laboratories. Characterization of impressed current technique was the main focus of this research,which involved determination of optimum chloride content and minimum immersion time of specimens for which the application of Faraday’s law could be efficient. To obtain optimum chloride content, the electrolytes in the corrosion cell were prepared similar to that of concrete pore solutions. Concrete prisms of 200 mm by 200 mm by 300 mm were used to determine the minimum immersion time for saturation. It was found that the optimum chloride content was 35 gm/L and the minimum immersion time for saturation was 140 hours. Accounting the results, a modified expression based on Faraday’s law was proposed to calculate weight loss due to corrosion. Journal of Engineering Science 11(1), 2020, 93-99

The Determination of Sense via Deleuze and Blanchot: Paradoxes of the Habitual, the Immemorial, and the Eternal Return

2008 ◽  
Vol 2 (2) ◽  
pp. 155-177 ◽  
Author(s):  
Eugene Brently Young
Keyword(s):  
Eternal Return

Eternal return is the paradox that accounts for the interplay between difference and repetition, a dynamic at the heart of Deleuze's philosophy, and Blanchot's approach to this paradox, even and especially through what it elides, further illuminates it. Deleuze draws on Blanchot's characterisations of difference, forgetting, and the unlivable to depict the ‘sense’ produced via eternal return, which, for Blanchot, is where repetition implicates or ‘carries’ pure difference. However, for Deleuze, difference and the unlivable are also developed by the living repetition or ‘contraction’ of habit, which results in his distinctive characterization of ‘force’, ‘levity’, and sense in eternal return.

Determination of the Principal Axes for Granite by Using the Polyhedral Specimen for the Purpose of the Characterization of the Microcrack Distribution

2008 ◽  
Vol 124 (12) ◽  
pp. 725-732 ◽  
Author(s):  
Yoshitaka NARA ◽  
Harumi KATO ◽  
Katsuhiko KANEKO
Keyword(s):  
Principal Axes

SEM/EDS Characterization of Uncommon Metal Whiskers and Determination of Underlying Growth Mechanisms

2018 ◽  
Author(s):  
D. Basak ◽  
L. H. Ponce
Keyword(s):  
Reliability Analysis ◽  
Case Studies ◽  
Whisker Growth ◽  
Innovation Systems ◽  
Growth Mechanisms ◽  
Crystal Oscillator ◽  
Analysis Laboratory ◽  
Investigative Techniques

Abstract Two case-studies on uncommon metals whiskers, performed at the Reliability Analysis Laboratory (RAL) of Northrop Grumman Innovation Systems, are presented. The components analyzed are an Oven Controlled Crystal Oscillator (OCXO) and an Electromechanical Relay. Investigative techniques were used to determine the chemical and physical makeup of the metal whiskers and develop an understanding of the underlying effects and mechanisms that caused the conditions conducive to whisker growth.

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