Desaturation of Myristoyl-CoA to Myristoleoyl-CoA by Hen Liver Microsomal Δ9-Desaturase

2004 ◽  
Vol 52 (13) ◽  
pp. 4234-4239
Author(s):  
Aziz C. Awad ◽  
Han-Seung Shin ◽  
Dale R. Romsos ◽  
J. Ian Gray
Keyword(s):  
Δ9 Desaturase ◽  
Myristoyl Coa

Direct Desaturation of Free Myristic Acid by Hen Liver Microsomal Δ9-Desaturase without Prior Activation to Myristoyl-CoA Derivative

2004 ◽  
Vol 52 (10) ◽  
pp. 3194-3201 ◽  
Author(s):  
Aziz C. Awad ◽  
Han-Seung Shin ◽  
Dale R. Romsos ◽  
J. Ian Gray
Keyword(s):  
Myristic Acid ◽  
Δ9 Desaturase ◽  
Myristoyl Coa

scholarly journals A non-canonical Δ9-desaturase synthesizing palmitoleic acid identified in the thraustochytrid Aurantiochytrium sp. T66

2021 ◽  
Author(s):  
E-Ming Rau ◽  
Inga Marie Aasen ◽  
Helga Ertesvåg
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Palmitoleic Acid ◽  
Unsaturated Fatty Acids ◽  
Gene Annotation ◽  
Vaccenic Acid ◽  
Strain Engineering ◽  
Δ9 Desaturase ◽  
Δ12 Desaturase

Abstract Thraustochytrids are oleaginous marine eukaryotic microbes currently used to produce the essential omega-3 fatty acid docosahexaenoic acid (DHA, C22:6 n-3). To improve the production of this essential fatty acid by strain engineering, it is important to deeply understand how thraustochytrids synthesize fatty acids. While DHA is synthesized by a dedicated enzyme complex, other fatty acids are probably synthesized by the fatty acid synthase, followed by desaturases and elongases. Which unsaturated fatty acids are produced differs between different thraustochytrid genera and species; for example, Aurantiochytrium sp. T66, but not Aurantiochytrium limacinum SR21, synthesizes palmitoleic acid (C16:1 n-7) and vaccenic acid (C18:1 n-7). How strain T66 can produce these fatty acids has not been known, because BLAST analyses suggest that strain T66 does not encode any Δ9-desaturase-like enzyme. However, it does encode one Δ12-desaturase-like enzyme. In this study, the latter enzyme was expressed in A. limacinum SR21, and both C16:1 n-7 and C18:1 n-7 could be detected in the transgenic cells. Our results show that this desaturase, annotated T66Des9, is a Δ9-desaturase accepting C16:0 as a substrate. Phylogenetic studies indicate that the corresponding gene probably has evolved from a Δ12-desaturase-encoding gene. This possibility has not been reported earlier and is important to consider when one tries to deduce the potential a given organism has for producing unsaturated fatty acids based on its genome sequence alone. Key points • In thraustochytrids, automatic gene annotation does not always explain the fatty acids produced. • T66Des9 is shown to synthesize palmitoleic acid (C16:1 n-7). • T66des9 has probably evolved from Δ12-desaturase-encoding genes.

scholarly journals Plastidial ACP Δ9‐desaturase modulates EPA biosynthesis and TAG accumulation in Phaeodactylum tricornutum

2021 ◽  
Author(s):  
Richard Smith ◽  
Juliette Jouhet ◽  
Chiara Gandini ◽  
Vladimir Nekrasov ◽  
Eric Marechal ◽  
...  
Keyword(s):  
Phaeodactylum Tricornutum ◽  
Δ9 Desaturase ◽  
Tag Accumulation

Dietary cholesterol and/or n − 3 fatty acid modulate Δ9-desaturase activity in rat liver microsomes

1988 ◽  
Vol 962 (3) ◽  
pp. 330-336 ◽  
Author(s):  
Manohar L. Garg ◽  
Antoni A. Wierzbicki ◽  
Alan B.R. Thomson ◽  
M.Thomas Clandinin
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Desaturase Activity ◽  
Liver Microsomes ◽  
Dietary Cholesterol ◽  
Rat Liver Microsomes ◽  
Δ9 Desaturase

Evidence for the up-regulation of stearoyl-ACP (Δ9) desaturase gene expression during cold acclimation

2004 ◽  
Vol 81 (2) ◽  
pp. 125-135 ◽  
Author(s):  
Sandra E. Vega ◽  
Alfonso H. del Rio ◽  
John B. Bamberg ◽  
Jiwan P. Palta
Keyword(s):  
Gene Expression ◽  
Cold Acclimation ◽  
Desaturase Gene ◽  
Δ9 Desaturase

scholarly journals Conjugated Linoleic Acid Is Synthesized Endogenously in Lactating Dairy Cows by Δ9-Desaturase

2000 ◽  
Vol 130 (9) ◽  
pp. 2285-2291 ◽  
Author(s):  
J. M. Griinari ◽  
B. A. Corl ◽  
S. H. Lacy ◽  
P. Y. Chouinard ◽  
K. V. V. Nurmela ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Lactating Dairy Cows ◽  
Δ9 Desaturase

Mammalian myristoyl CoA: protein N-myristoyltransferase

1995 ◽  
pp. 191-202
Author(s):  
Rajala V. S. Raju ◽  
Bernadene A. Magnuson ◽  
Rajendra K. Sharma
Keyword(s):  
Myristoyl Coa

Substrate specificity of acyl-lipid Δ9-desaturase from Prochlorothrix hollandica cyanobacterium producing myristoleic acid

2017 ◽  
Vol 64 (4) ◽  
pp. 560-565 ◽  
Author(s):  
A. Yu. Starikov ◽  
A. A. Userbaeva ◽  
S. S. Lapina ◽  
K. S. Mironov ◽  
I. P. Maslova ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Acyl Lipid ◽  
Δ9 Desaturase

The role of Δ9-desaturase in the production of cis-9, trans-11 CLA

2001 ◽  
Vol 12 (11) ◽  
pp. 622-630 ◽  
Author(s):  
Benjamin A. Corl ◽  
Lance H. Baumgard ◽  
Debra A. Dwyer ◽  
J.Mikko Griinari ◽  
Bliss S. Phillips ◽  
...  
Keyword(s):  
Δ9 Desaturase
Sign in / Sign up

Export Citation Format

Share Document