Eicosapentaenoic Acid Enrichment from Sardine Oil by Argentation Chromatography

Sardine fish meal by-product contain eicosapentaenoic acid (EPA) and docosahexaenoic (DHA) and it can be made as emulsion. The purpose of this study were to determine the best fish oil emulsion by mixing<br />the oil phase (lecithin 3% and oil) and water phase (carboxymethyl cellulose/CMC 2% and fruit juice) and then stored until creaming, and the emulsion is analyzed their viscosity, pH, percent of stability and long<br />separation. Sardine oil is separated from the emulsion and tested oxidation parameters. The best emulsion was fish oil emulsion after refined without citric acid (RTS) with viscosity (2470.31 cP), pH (5.64), percent of stability (56.14%) and long separation (14 days). Primary and secondary oxidation parameters of RTS were FFA (14.87%), PV (14.43 meq/kg), AV (32.57 meq KOH/g), AnV (17.3 meq/kg), and Totox (46.16 meq/kg).

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Obtention of highly purified fractions of eicosapentaenoic acid and docosahexaenoic acid from sardine oil by silver-resin chromatography. A semipreparative procedure.

Grasas y Aceites ◽

10.3989/gya.1997.v48.i4.789 ◽

1997 ◽

Vol 48 (4) ◽

pp. 197-199 ◽

Cited By ~ 2

Author(s):

S. Nieto ◽

Ana M. Córdoba ◽

J. Sanhueza ◽

A. Valenzuela

Keyword(s):

Docosahexaenoic Acid ◽

Eicosapentaenoic Acid ◽

Sardine Oil

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Eicosapentaenoic acid

Lipidomics Gateway ◽

10.1038/lipidmaps.2011.5 ◽

2011 ◽

Author(s):

Samia Burridge

Keyword(s):

Eicosapentaenoic Acid

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Eicosapentaenoic Acid Interferes with U46619-Stimulated Formation of Inositol Phosphates in Washed Rabbit Platelets

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647129 ◽

1989 ◽

Vol 62 (04) ◽

pp. 1116-1120 ◽

Cited By ~ 9

Author(s):

N Chetty ◽

J D Vickers ◽

R L Kinlough-Rathbone ◽

M A Packham ◽

J F Mustard

Keyword(s):

Signal Transduction ◽

Fatty Acid Composition ◽

Eicosapentaenoic Acid ◽

Inositol Phosphates ◽

Inositol Phosphate ◽

Dense Granule ◽

Detectable Change ◽

Membrane Phospholipids ◽

Rabbit Platelets ◽

Stimulation Of

SummaryEicosapentaenoic acid (EPA) inhibits platelet responsiveness to aggregating agents. To investigate the reactions that are affected by EPA, we examined the effect of preincubating aspirintreated rabbit platelets with EPA on stimulation of inositol phosphate formation in response to the TXA2 analogue U46619. Stimulation of platelets with U46619 (0.5 μM) caused aggregation and slight release of dense granule contents; aggregation and release were inhibited by preincubation of the platelets with EPA (50 μM) for 1 h followed by washing to remove unincorporated EPA. Incubation with EPA (50 μM) for 1 h did not cause a detectable increase in the amount of EPA in the platelet phospholipids. When platelets were prelabelled with [3H]inositol stimulation with U46619 of control platelets that had not been incubated with EPA significantly increased the labelling of mos1tol phosphates. The increases in inositol phosphate labelling due to U46619 at 10 and 60 s were partially inhibited by premcubat10n of the platelets with 50 μM EPA. Since the activity of cyclo-oxygenase was blocked with aspirin, inhibition of inositol phosphate labelling in response to U46619 indicates either that there may be inhibition of signal transduction without a detectable change in the amount of EPA in platelet phospholipids, that changes in signal transduction require only minute changes in the fatty acid composition of membrane phospholipids, or that after a 1 h incubation with EPA, activation of phospholipase C is affected by a mechanism that is not directly related to incorporation of EPA.

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