Chemo-enzymatic synthesis of Lacto-N-biose I catalyzed by β-1,3 galactosidase from Bacillus circulans using 4,6-dimethoxy-1,3,5-triazin-2-yl β-galactopyranoside as a glycosyl donor

Chemo-enzymatic synthesis of lacto-N-biose I (LNB) catalyzed by β-1,3 galactosidase from Bacillus circulans (BgaC) has been developed using 4,6-dimethoxy-1,3,5-triazin-2-yl β-galactopyranoside [DMT-β-Gal] and GlcNAc as the donor and acceptor substrates, respectively. BgaC transferred the Gal moiety to the acceptor, giving rise to LNB. The maximum yield of LNB was obtained at the acceptor: donor substrate ratio of 1:30.

Bacillus circulans GN03 Alters the Microbiota, Promotes Cotton Seedling Growth and Disease Resistance, and Increases the Expression of Phytohormone Synthesis and Disease Resistance-Related Genes

Plant growth-promoting bacteria (PGPB) are components of the plant rhizosphere that promote plant growth and/or inhibit pathogen activity. To explore the cotton seedlings response to Bacillus circulans GN03 with high efficiency of plant growth promotion and disease resistance, a pot experiment was carried out, in which inoculations levels of GN03 were set at 104 and 108 cfu⋅mL–1. The results showed that GN03 inoculation remarkably enhanced growth promotion as well as disease resistance of cotton seedlings. GN03 inoculation altered the microbiota in and around the plant roots, led to a significant accumulation of growth-related hormones (indole acetic acid, gibberellic acid, and brassinosteroid) and disease resistance-related hormones (salicylic acid and jasmonic acid) in cotton seedlings, as determined with ELISA, up-regulated the expression of phytohormone synthesis-related genes (EDS1, AOC1, BES1, and GA20ox), auxin transporter gene (Aux1), and disease-resistance genes (NPR1 and PR1). Comparative genomic analyses was performed between GN03 and four similar species, with regards to phenotype, biochemical characteristics, and gene function. This study provides valuable information for applying the PGPB alternative, GN03, as a plant growth and disease-resistance promoting fertilizer.

The Impact of Potassium Sources and Bio Fertilizer on Corn Plant and Potassium Availability in Calcareous Soil

Two field experiments were conducted in a calcareous soil during summer seasons of 2019 and 2020 at the experimental in a private farm Mallawi, El- Minia Governorate, Egypt to evaluate application of K fertilization at different rates of K2SO4 and non-classic products, i.e. K feldspar, mixture with or without inoculation with the K dissolving bacteria (Bacillus circulans.) soil chemical properties, plant growth, yield and nutritional value of corn (Zea-maize hybrid third 310). Our results proved that inoculation of maize seeds with Bacillus circulans at rate of 36% K2O improved soil pH, EC, soil organic matter content and enhanced the soil available N, P and K concentrations. Also, the growth parameters, yield and nutritional status of the plants were significantly increased by using non-traditional potassium fertilizers particularly in the case of seed inoculated with potassium dissolving bacteria (Bacillus circulans).