Reliable Enzyme-Linked Immunosorbent Assay for the Determination of Soybean Proteins in Processed Foods

2008 ◽  
Vol 56 (16) ◽  
pp. 6818-6824 ◽  
Author(s):  
Naoki Morishita ◽  
Kumiko Kamiya ◽  
Takashi Matsumoto ◽  
Shinobu Sakai ◽  
Reiko Teshima ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Foods ◽  
Soybean Proteins

scholarly journals Interlaboratory Evaluation of Two Enzyme-Linked Immunosorbent Assay Kits for the Determination of Crustacean Protein in Processed Foods

2008 ◽  
Vol 91 (1) ◽  
pp. 123-129 ◽  
Author(s):  
Shinobu Sakai ◽  
Rieko Matsuda ◽  
Reiko Adachi ◽  
Hiroshi Akiyama ◽  
Tamio Maitani ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Immunosorbent Assay ◽  
Soluble Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Foods ◽  
Relative Standard ◽  
High Level

Abstract The labeling of foods containing material derived from crustaceans such as shrimp and crab is to become mandatory in Japan because of increases in the number of allergy patients. To ensure proper labeling, 2 novel sandwich enzyme-linked immunosorbent assay (ELISA) kits for the determination of crustacean protein in processed foods, the N kit (Nissui Pharmaceutical Co., Ltd, Ibaraki, Japan) and the M kit (Maruha Nichiro Holdings, Inc., Ibaraki, Japan), have been developed. Five types of model processed foods containing 10 and/or 11.9 g/g crustacean soluble protein were prepared for interlaboratory evaluation of the performance of these kits. The N kit displayed a relatively high level of reproducibility relative standard deviation (interlaboratory precision; 4.08.4 RSDR) and sufficient recovery (6586) for all the model processed foods. The M kit displayed sufficient reproducibility (17.620.5 RSDR) and a reasonably high level of recovery (82103). The repeatability relative standard deviation (RSDr) values regarding the detection of crustacean proteins in the 5 model foods were mostly <5.1 RSDr for the N kit and 9.9 RSDr for the M kit. In conclusion, the results of this interlaboratory evaluation suggest that both these ELISA kits would be very useful for detecting crustacean protein in processed foods.

Reliable Enzyme-Linked Immunosorbent Assay for the Determination of Coconut Milk Proteins in Processed Foods

2011 ◽  
Vol 59 (6) ◽  
pp. 2131-2136 ◽  
Author(s):  
Vipa Surojanametakul ◽  
Hirotoshi Doi ◽  
Haruki Shibata ◽  
Tasuku Mizumura ◽  
Toshio Takahashi ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Milk Proteins ◽  
Coconut Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Foods

Reliable Enzyme-Linked Immunosorbent Assay for the Determination of Walnut Proteins in Processed Foods

2008 ◽  
Vol 56 (17) ◽  
pp. 7625-7630 ◽  
Author(s):  
Hirotoshi Doi ◽  
Yuki Touhata ◽  
Haruki Shibata ◽  
Shinobu Sakai ◽  
Atsuo Urisu ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Foods

scholarly journals Enzyme-Linked Immunosorbent Assay Kit for the Determination of Soybean Protein in Processed Foods: Interlaboratory Evaluation

2010 ◽  
Vol 93 (1) ◽  
pp. 243-248 ◽  
Author(s):  
Shinobu Sakai ◽  
Reiko Adachi ◽  
Hiroshi Akiyama ◽  
Reiko Teshima ◽  
Naoki Morishita ◽  
...  
Keyword(s):  
Soybean Protein ◽  
Sandwich Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Foods ◽  
Adzuki Bean ◽  
Soybean Proteins ◽  
Elisa Kit ◽  
Number Of Patients ◽  
High Level

Abstract The labeling of foods containing ingredients derived from soybean is recommended in Japan because of an increasing number of patients who are allergic to soybeans. To ensure proper labeling, a novel sandwich ELISA kit for the determination of soybean protein in processed foods (FASTKIT Ver. II, Soybean, Nippon Meat Packers, Inc.; soy kit) has been developed. Five types of incurred samples (model processed foods: rice gruel, sausage, sweet adzuki bean soup, sweet potato cake, and tomato sauce) containing 10 g soybean soluble protein/g food were prepared for use in interlaboratory evaluations of the soy kit. The soy kit displayed a sufficient RSDR value (interlaboratory precision: 9.313.4 RSDR) and a high level of recovery (97114) for all the incurred samples. The RSDr value for the incurred samples was mostly <4.8. The results of this interlaboratory evaluation suggest that the soy kit can be used as a precise and reliable tool for the determination of soybean proteins in processed foods.

A Reliable Enzyme Linked Immunosorbent Assay for the Determination of Bovine and Porcine Gelatin in Processed Foods

2009 ◽  
Vol 57 (5) ◽  
pp. 1721-1726 ◽  
Author(s):  
Hirotoshi Doi ◽  
Eriko Watanabe ◽  
Haruki Shibata ◽  
Soichi Tanabe
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Foods

A sensitive enzyme-linked immunosorbent assay for the determination of fish protein in processed foods

2013 ◽  
Vol 136 (2) ◽  
pp. 675-681 ◽  
Author(s):  
Yusuke Shibahara ◽  
Yoshihiko Uesaka ◽  
Jun Wang ◽  
Shoichi Yamada ◽  
Kazuo Shiomi
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Processed Foods ◽  
Fish Protein

SENSITIVE AND SPECIFIC ENZYME-LINKED IMMUNOSORBENT ASSAY FOR UROKINASE-TYPE PLASMINOGEN ACTIVATOR IN HUMAN PLASMA

1987 ◽  
Author(s):  
J Grøndahl-HANSEN ◽  
N Agerlin ◽  
L S Nielsen ◽  
K Danø
Keyword(s):  
Plasminogen Activator ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mean Values ◽  
Amino Terminal ◽  
Type Plasminogen Activator ◽  
Healthy Donors ◽  
Urokinase Type Plasminogen Activator ◽  
The Mean

An enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of human urokinase-type plasminogen activator (u-PA) in plasma and serum. Microtiter plates were coated with a monoclonal antibody and incubated with standard or sample. Bound u-PA was quantitated with polyclonal antibodies conjugated with biotin, followed by avidin-peroxidase. The assay was 10-fold as sensitive as other previously reported ELISAs, the detection limit being approximately 1 pg of u-PA in a volume of 100 μl with a linear dose-response up to 15 pg of u-PA. The assay detected active u-PA and its inactive proenzyme form equally well and the recovery of both forms was higher than 90% in plasma. A variety of structurally related proteins, including t-PA, were tested, but no reaction with proteins other than u-PA and its amino-terminal degradation product were observed. The intra-assay and inter-assay coefficients of variation for determination of u-PA in plasma were 7.6% and 8.4%, respectively. The assay was equally applicable to serum. The values obtained with plasma and serum were similar, and the results were not affected by small variations in the preparation of the samples. The ELISA was used to measure the concentration of u-PA in plasma from 34 healthy donors. The mean values for u-PA in plasma from healthy donors was 1.1 ng/ml ± 0.3 ng/ml (SD) (range 0.6 - 1.5 ng/ml). No significant differences were found between men and women and no correlation between u-PA concentration and age could be demonstrated.The mean u-PA concentration in plasma from healthy donors obtained in this study is substantially lower than that reported by others. This might be due to different methods of determination of the protein content of the standard preparations or to differences in the specificity of the assays.

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