Rapid Determination of the Geographical Origin of Honey Based on Protein Fingerprinting and Barcoding Using MALDI TOF MS

2009 ◽  
Vol 57 (21) ◽  
pp. 10081-10088 ◽  
Author(s):  
Jun Wang ◽  
Michael M. Kliks ◽  
Weiyue Qu ◽  
Soojin Jun ◽  
Gongyi Shi ◽  
...  
2000 ◽  
Vol 48 (2) ◽  
pp. 150-154 ◽  
Author(s):  
Yoichiro NIHASHI ◽  
Mitsuhiro ISHIDA ◽  
Fumio SAKAMOTO ◽  
Yuichiro NAKADA
Keyword(s):  

2019 ◽  
Vol 411 (22) ◽  
pp. 5833-5843 ◽  
Author(s):  
Missael Antonio Arroyo Negrete ◽  
Kazimierz Wrobel ◽  
Eunice Yanez Barrientos ◽  
Alma Rosa Corrales Escobosa ◽  
Francisco Javier Acevedo Aguilar ◽  
...  

2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Michael A Reeve ◽  
Denise Bachmann

Abstract Analytical techniques currently available for the characterization of mixtures of microorganisms are generally based on next-generation sequencing. Motivated to develop practical and less-expensive methods for characterizing such mixtures, we propose, as an alternative or complement, the use of matrix-assisted laser-desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS), which is capable of high-resolution discrimination between species and even between biotypes within species. Potential approaches employing this technique for such characterization are discussed along with impediments to their successful employment. As a consequence, our rationale has been to capitalize on the powerful algorithms currently available for spectral comparison. Following this rationale, the first priority is to ensure the generation of MALDI-TOF MS spectra from mixtures of microorganisms that contain manageable peak complexities and that can be handled by the existing spectral comparison algorithms, preferably with the option to archive and re-run sample preparations and to pipette replicates of these onto MALDI-TOF MS sample plates. The second priority is to ensure that database entry is comparably facile to sample preparation so that large databases of known microorganism mixture MALDI-TOF MS spectra could be readily prepared for comparison with the spectra of unknown mixtures. In this article, we address the above priorities and generate illustrative MALDI-TOF MS spectra to demonstrate the utility of this approach. In addition, we investigate methods aimed at chemically modulating the peak complexity of the obtained MALDI-TOF MS spectra.


2011 ◽  
Vol 47 (35) ◽  
pp. 9816 ◽  
Author(s):  
Xiao-Shui Li ◽  
Jian-Hong Wu ◽  
Li-Dan Xu ◽  
Qin Zhao ◽  
Yan-Bo Luo ◽  
...  

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