Thin Film Voltammetry of Wild Type and Mutant Reaction Center Proteins from Photosynthetic Bacteria

2011 ◽  
Vol 115 (12) ◽  
pp. 3226-3232 ◽  
Author(s):  
Yun Zhang ◽  
Amy M. LaFountain ◽  
Nikki Magdaong ◽  
Marcel Fuciman ◽  
James P. Allen ◽  
...  
Keyword(s):  
Thin Film ◽  
Reaction Center ◽  
Photosynthetic Bacteria ◽  
Wild Type

CHLOROPHYLL-PROTEINS AND REACTION CENTER PREPARATIONS FROM PHOTOSYNTHETIC BACTERIA, ALGAE AND HIGHER PLANTS,‡

1971 ◽  
Vol 14 (3) ◽  
pp. 329-341 ◽  
Author(s):  
J. PHILIP THORNBER ◽  
JOHN M. OLSON
Keyword(s):  
Reaction Center ◽  
Photosynthetic Bacteria ◽  
Higher Plants

Photoconverters with organic semiconductors and photosynthetic bacteria: positioning the bacterial Reaction Center in nanostructures

2016 ◽  
Author(s):  
Gianluca M. Farinola ◽  
Roberta Ragni ◽  
Francesco Milano ◽  
Simona La Gatta ◽  
Roberto R. Tangorra ◽  
...  
Keyword(s):  
Reaction Center ◽  
Organic Semiconductors ◽  
Photosynthetic Bacteria ◽  
Bacterial Reaction Center

scholarly journals Silicon-based Biosensors for Rapid Detection of Protein or Nucleic Acid Targets

2001 ◽  
Vol 47 (10) ◽  
pp. 1894-1900 ◽  
Author(s):  
Robert Jenison ◽  
Helen La ◽  
Ayla Haeberli ◽  
Rachel Ostroff ◽  
Barry Polisky
Keyword(s):  
Thin Film ◽  
Nucleic Acid ◽  
Interferon Γ ◽  
Wild Type ◽  
Signal To Noise ◽  
Cytokine Detection ◽  
Apparent Color ◽  
Multianalyte Detection ◽  
A Charge ◽  
Silicon Based

Abstract Background: We developed a silicon-based biosensor that generates visual, qualitative results or quantitative results for the detection of protein or nucleic acid targets in a multiplex format. Methods: Capture probes were immobilized either passively or covalently on the optically coated surface of the biosensor. Intermolecular interactions of the immobilized capture probe with specific target molecules were transduced into a molecular thin film. Thin films were generated by enzyme-catalyzed deposition in the vicinity of the surface-bound target. The increased thickness on the surface changed the apparent color of the biosensor by altering the interference pattern of reflected light. Results: Cytokine detection was achieved in a 40-min multiplex assay. Detection limits were 4 ng/L for interleukin (IL)-6, 31 ng/L for IL1-β, and 437 ng/L for interferon-γ. In multianalyte experiments, cytokines were specifically detected with signal-to-noise ratios ranging from 15 to 80. With a modified optical surface, specificity was also demonstrated in a nucleic acid array with unambiguous discrimination of single-base changes in a 15-min assay. For homozygous wild-type and homozygous mutant samples, signal-to-noise ratios of ∼100 were observed. Heterozygous samples yielded approximately equivalent signals for wild-type and mutant capture probes. Conclusions: The thin-film biosensor allows rapid, sensitive, and specific detection of protein or nucleic acid targets in an array format with results read visually or quantified with a charge-coupled device camera. This biosensor is suited for multianalyte detection in clinical diagnostic assays.

scholarly journals M234Glu is a component of the proton sponge in the reaction center from photosynthetic bacteria

2009 ◽  
Vol 1787 (12) ◽  
pp. 1505-1515 ◽  
Author(s):  
Hélène Cheap ◽  
Sophie Bernad ◽  
Valérie Derrien ◽  
László Gerencsér ◽  
Julia Tandori ◽  
...  
Keyword(s):  
Reaction Center ◽  
Photosynthetic Bacteria ◽  
Proton Sponge

Isolation and partial characterization of a P870 reaction center complex from wild type Rhodospirillum rubrum

1972 ◽  
Vol 275 (2) ◽  
pp. 219-230 ◽  
Author(s):  
Henri Noël ◽  
Michel van der Rest ◽  
Gabriel Gingras
Keyword(s):  
Reaction Center ◽  
Rhodospirillum Rubrum ◽  
Partial Characterization ◽  
Wild Type ◽  
Reaction Center Complex
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